Nanopore basecalling.

Raw signal. Ready to discover.

Upload POD5 files, or connect your sequencing folder.

Connect to beginNVIDIA B300 · HAC / SUP

Sign in to start basecalling

Your files and results are private to your Archaions account.

Your reads. A clearer beginning.

Basecall on NVIDIA B300 with HAC or SUP.

Run nameSequencing kitBasecalling modelServer GPU

HAC / SUP · FLO-PRO114M / SQK-LSK114 at 5 kHz

Modified bases

Optional. Choose one model per base; adenine and cytosine calls can run together. Modification probabilities are retained in BAM.

Demultiplexing & trimming Split results by barcode

Choose a barcoding kit to enable demultiplexing.

Require barcode at both ends Trim adapters, primers and barcodes where supported

Quality control Generate QC report

Minimum read Q-scoreMinimum read length (bases)

Zero keeps all reads. Filters apply to BAM, FASTQ and barcode outputs. The QC report summarizes reads before filtering and reports pass/fail counts.

Choose POD5 filesor drop them hereMultiple files · up to 2 GiB each

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Your computer records. The server basecalls.

In MinKNOW, save POD5 output and switch off local basecalling if you want the server to handle it.

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—Basecalled reads

Follow each file from upload to basecalling, then download BAM and FASTQ results.