Paste a full-length rRNA sequence (50-5000 bp). Predictions are returned across all eight taxonomic ranks with confidence scores. Try a Bacterial example.

Sequence

Result

Classification

Enter a public NCBI SRA run accession and we fetch the reads for you, or upload your own FASTA/FASTQ file (gzipped supported). Each sequence is classified individually and a community-level abundance summary is provided. Maximum 150 MB.

NCBI SRA / ENA run accession

or

File

Drop your file here or click to browse

.fasta · .fa · .fastq · .fq · .gz

Limit sample size Off by default — all reads in the file are processed. Turn on to cap how many reads to process (e.g. a faster preview).

Sample size Number of reads to process. No maximum — enter any value.

Skip adapter trimming Adapter trimming now runs automatically when ONT adapter signatures are detected in more than 5% of sampled reads. Enable this only if your reads are already adapter-trimmed.

Minimum quality (FASTQ only) Phred score threshold. 0 disables filtering. 7 is the permissive Nanopore default.

0

Custom length filter Off by default — reads are filtered to the marker's validated window (16S 1,200–2,000 bp, ITS 300–1,000 bp). Turn on to set your own range.

Length range (bp) Reads outside this min–max are discarded before classification.

–

Uploading… 0%

Processing pipeline

Result

Taxonomic abundance

Min abundance — table shows top 25; CSV export is always full

AI Generated summary

Combine up to 10 samples — public SRA runs, your own FASTA/FASTQ files, or a mix (1 GB total) — and add metadata for downstream comparison. Metadata can be entered in the table or uploaded as a CSV/XLSX with a filename column (an accession matches a row named after it).

NCBI SRA / ENA run accessions

or

Files

Drop multiple files here or click to browse

Skip adapter trimming Adapter trimming runs automatically for each sample when ONT adapter signatures are detected in more than 5% of sampled reads. Enable this only if your reads are already adapter-trimmed.

Limit sample size Off by default — all reads in every sample are processed. Turn on to cap how many reads per sample.

Sample size (per sample) Number of reads to process per sample. No maximum — enter any value.

Custom length filter Off by default — every sample is filtered to the marker's validated window (16S 1,200–2,000 bp, ITS 300–1,000 bp). Turn on to set your own range.

Length range (bp) Reads outside this min–max are discarded before classification.

–

Processing samples

Result

Multi-sample analysis

Export — CSV exports are always the full table, not the top 25

Sample

Taxonomic rank

Show top

Leaf rank

Min abundance %

Taxonomic classification for full-length rRNA Nanopore sequences

Submit a sequence or sequencing dataset