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archAIons · MetaBiome Project · Oral · Public research data

SRR13679659

Metagenome or environmental sample from human oral metagenome

BioSample: SAMN17774963 · Study: PRJNA699316 · Body site: saliva

Interactive report · NCBI SRA · ENA

69Reported species
9Reported genera

Taxonomic abundance

Relative abundance is normalized among reported taxa. No reported taxa does not establish absence of organisms.

SpeciesGenusPhylumAbundance
Streptococcus salivariusStreptococcusBacillota16.72587%
Haemophilus parainfluenzaeHaemophilusPseudomonadota11.22766%
Veillonella parvulaVeillonellaBacillota8.39852%
Streptococcus anginosusStreptococcusBacillota8.00379%
Streptococcus pneumoniaeStreptococcusBacillota5.58823%
Streptococcus downeiStreptococcusBacillota5.07195%
Streptococcus gordoniiStreptococcusBacillota4.49660%
Haemophilus influenzaeHaemophilusPseudomonadota4.17208%
Veillonella disparVeillonellaBacillota3.59826%
Streptococcus mitisStreptococcusBacillota3.40678%
Streptococcus sanguinisStreptococcusBacillota2.95742%
Veillonella rodentiumVeillonellaBacillota2.94086%
Campylobacter concisusCampylobacterPseudomonadota1.53009%
Streptococcus pyogenesStreptococcusBacillota1.51708%
Streptococcus oralisStreptococcusBacillota1.32986%
Gemella palaticanisGemellaBacillota1.26303%
Gemella haemolysansGemellaBacillota1.10881%
Haemophilus aegyptiusHaemophilusPseudomonadota1.09695%
Granulicatella balaenopteraeGranulicatellaBacillota0.96995%
Campylobacter curvusCampylobacterPseudomonadota0.81918%
Haemophilus pittmaniaeHaemophilusPseudomonadota0.70987%
Veillonella infantiumVeillonellaBacillota0.70901%
Campylobacter sputorumCampylobacterPseudomonadota0.65854%
Neisseria meningitidisNeisseriaPseudomonadota0.62814%
Granulicatella adiacensGranulicatellaBacillota0.52567%
Streptococcus himalayensisStreptococcusBacillota0.52465%
Streptococcus parasanguinisStreptococcusBacillota0.51677%
Veillonella seminalisVeillonellaBacillota0.50901%
Gemella parahaemolysansGemellaBacillota0.48637%
Streptococcus marmotaeStreptococcusBacillota0.44421%
Haemophilus haemoglobinophilusHaemophilusPseudomonadota0.43253%
Granulicatella elegansGranulicatellaBacillota0.40249%
Haemophilus haemolyticusHaemophilusPseudomonadota0.39758%
Streptococcus constellatusStreptococcusBacillota0.35298%
Streptococcus periodonticumStreptococcusBacillota0.32295%
Neisseria dentiaeNeisseriaPseudomonadota0.30748%
Streptococcus intermediusStreptococcusBacillota0.29942%
Haemophilus parahaemolyticusHaemophilusPseudomonadota0.29685%
Neisseria gonorrhoeaeNeisseriaPseudomonadota0.27694%
Neisseria elongataNeisseriaPseudomonadota0.26665%
Neisseria flavescensNeisseriaPseudomonadota0.25829%
Gemella morbillorumGemellaBacillota0.25322%
Streptococcus cristatusStreptococcusBacillota0.23836%
Gemella asaccharolyticaGemellaBacillota0.23370%
Haemophilus paraphrohaemolyticusHaemophilusPseudomonadota0.23256%
Haemophilus sputorumHaemophilusPseudomonadota0.23196%
Streptococcus infantisStreptococcusBacillota0.20949%
Streptococcus sinensisStreptococcusBacillota0.20585%
Fusobacterium nucleatumFusobacteriumFusobacteriota0.19914%
Streptococcus alactolyticusStreptococcusBacillota0.19357%
Prevotella melaninogenicaPrevotellaBacteroidota0.17372%
Campylobacter hyointestinalisCampylobacterPseudomonadota0.16578%
Streptococcus pluranimaliumStreptococcusBacillota0.16428%
Streptococcus sobrinusStreptococcusBacillota0.15309%
Neisseria lactamicaNeisseriaPseudomonadota0.15169%
Neisseria subflavaNeisseriaPseudomonadota0.14530%
Streptococcus mutansStreptococcusBacillota0.13529%
Streptococcus thermophilusStreptococcusBacillota0.13000%
Veillonella atypicaVeillonellaBacillota0.12756%
Neisseria canisNeisseriaPseudomonadota0.11965%
Gemella cuniculiGemellaBacillota0.11937%
Neisseria polysacchareaNeisseriaPseudomonadota0.11741%
Streptococcus acidominimusStreptococcusBacillota0.11650%
Streptococcus koreensisStreptococcusBacillota0.11218%
Haemophilus massiliensisHaemophilusPseudomonadota0.11218%
Neisseria zoodegmatisNeisseriaPseudomonadota0.10971%
Gemella taiwanensisGemellaBacillota0.10836%
Streptococcus macacaeStreptococcusBacillota0.09738%
Streptococcus australisStreptococcusBacillota0.09337%

Reference-based 16S phylogeny

69 of 69 reported species have an eligible reference. MAFFT alignment and FastTree GTR+CAT tree, pruned to this sample. This is not a phylogeny inferred from sample reads or ASVs. Root placement is arbitrary; branch lengths are substitutions per site.

Streptococcus periodonticum0.32%Streptococcus anginosus8.00%Streptococcus constellatus0.35%Streptococcus intermedius0.30%Streptococcus cristatus0.24%Streptococcus sinensis0.21%Streptococcus parasanguinis0.52%Streptococcus infantis0.21%Streptococcus oralis1.33%Streptococcus mitis3.41%Streptococcus pneumoniae5.59%Streptococcus australis0.09%Streptococcus koreensis0.11%Streptococcus himalayensis0.52%Streptococcus marmotae0.44%Streptococcus gordonii4.50%Streptococcus sanguinis2.96%Streptococcus acidominimus0.12%Streptococcus alactolyticus0.19%Streptococcus pyogenes1.52%Granulicatella balaenopterae0.97%Granulicatella adiacens0.53%Granulicatella elegans0.40%Gemella asaccharolytica0.23%Gemella palaticanis1.26%Gemella cuniculi0.12%Gemella parahaemolysans0.49%Gemella taiwanensis0.11%Gemella haemolysans1.11%Gemella morbillorum0.25%Veillonella seminalis0.51%Veillonella dispar3.60%Veillonella infantium0.71%Veillonella parvula8.40%Veillonella atypica0.13%Veillonella rodentium2.94%Fusobacterium nucleatum0.20%Campylobacter sputorum0.66%Campylobacter curvus0.82%Campylobacter concisus1.53%Campylobacter hyointestinalis0.17%Neisseria canis0.12%Neisseria zoodegmatis0.11%Neisseria dentiae0.31%Neisseria subflava0.15%Neisseria lactamica0.15%Neisseria polysaccharea0.12%Neisseria meningitidis0.63%Neisseria gonorrhoeae0.28%Neisseria flavescens0.26%Neisseria elongata0.27%Haemophilus parainfluenzae11.23%Haemophilus sputorum0.23%Haemophilus parahaemolyticus0.30%Haemophilus paraphrohaemolyticus0.23%Haemophilus haemoglobinophilus0.43%Haemophilus haemolyticus0.40%Haemophilus aegyptius1.10%Haemophilus influenzae4.17%Haemophilus massiliensis0.11%Haemophilus pittmaniae0.71%Prevotella melaninogenica0.17%Streptococcus macacae0.10%Streptococcus mutans0.14%Streptococcus sobrinus0.15%Streptococcus downei5.07%Streptococcus pluranimalium0.16%Streptococcus salivarius16.73%Streptococcus thermophilus0.13%Branch length scale: 1.0238 substitutions/site across the 500-unit horizontal axis. Root arbitrary.

Public raw read files

Files contain the original full run. Downloads are hosted by ENA.

FileType / bytesMD5
SRR13679659_1.fastq.gzFASTQ / 2789621209d91bd96bd0a7a3cdd8061420cce2483

Analysis provenance

marker16S
pipelineArchaions v18.8
read_samplingFirst available reads, not random subsampling
input_sha256db8fa7711f32f102aa6d6f5d5cbab136d6a2763b66efc8043e8c9acf6172edc8
median_read_length1582.0
reads_in_length_window28235
length_window_bp[1200, 2000]
quality_scoresUnavailable in fetched FASTA; no measured quality filtering
classifier_stats{"marker": "16s", "pipeline": "v18.8", "gpu": "RTX-PRO-6000", "input_path": "volume-fastq-gz", "reads_in": 30000, "reads_skipped": 6312, "elapsed_seconds": 2.5462721850000207, "reads_submitted": 30000, "reads_after_filter": 30000, "adapter_scan": {"sampled": 200, "matched": 9, "fraction": 0.045, "adapters_found": {"27F": 9}, "should_trim": false}, "porechop_ran": false, "porechop_reason": "fast-path: no adapter trimming required"}

Full public metadata

All retrieved values are included. Blank source values are displayed as “Not reported”. Expand the sections below to inspect the full record.

ENA run fields (195)
run_accessionSRR13679659
ageNot reported
alignedNot reported
altitudeNot reported
assembly_qualityNot reported
assembly_softwareNot reported
bam_asperaNot reported
bam_bytesNot reported
bam_file_roleNot reported
bam_ftpNot reported
bam_galaxyNot reported
bam_md5Not reported
base_count281735875
binning_softwareNot reported
bio_materialNot reported
bisulfite_protocolNot reported
broad_scale_environmental_contextNot reported
broker_nameNot reported
cage_protocolNot reported
cell_lineNot reported
cell_typeNot reported
center_nameSUB9029329
checklistNot reported
chip_ab_providerNot reported
chip_protocolNot reported
chip_targetNot reported
collected_byNot reported
collection_date2019-11
collection_date_end2019-11-30
collection_date_start2019-11-01
completeness_scoreNot reported
contamination_scoreNot reported
control_experimentNot reported
countryUSA: Texas
cultivarNot reported
culture_collectionNot reported
datahubdcc_metagenome
depthNot reported
descriptionMinION sequencing: 16s RNA of human oral metagenome: conc 18
dev_stageNot reported
diseaseNot reported
dnase_protocolNot reported
ecotypeNot reported
elevationNot reported
environment_biomeNot reported
environment_featureNot reported
environment_materialNot reported
environmental_mediumNot reported
environmental_sampleNot reported
experiment_accessionSRX10069043
experiment_aliassaliva_conc_18
experiment_targetNot reported
experiment_titleMinION sequencing: 16s RNA of human oral metagenome: conc 18
experimental_factorNot reported
experimental_protocolNot reported
extraction_protocolNot reported
faang_library_selectionNot reported
fastq_asperafasp.sra.ebi.ac.uk:/vol1/fastq/SRR136/059/SRR13679659/SRR13679659_1.fastq.gz
fastq_bytes278962120
fastq_file_roleGENERATED_FILE
fastq_ftpftp.sra.ebi.ac.uk/vol1/fastq/SRR136/059/SRR13679659/SRR13679659_1.fastq.gz
fastq_galaxyftp.sra.ebi.ac.uk/vol1/fastq/SRR136/059/SRR13679659/SRR13679659_1.fastq.gz
fastq_md59d91bd96bd0a7a3cdd8061420cce2483
file_locationNot reported
first_created2022-03-03
first_public2022-03-03
germlineNot reported
hi_c_protocolNot reported
hostHomo sapiens
host_body_siteNot reported
host_genotypeNot reported
host_gravidityNot reported
host_growth_conditionsNot reported
host_phenotypeNot reported
host_scientific_nameHomo sapiens
host_sexNot reported
host_statusNot reported
host_tax_id9606
identified_byNot reported
instrument_modelMinION
instrument_platformOXFORD_NANOPORE
investigation_typeNot reported
isolateNot reported
isolation_sourcesaliva
last_updated2022-03-03
lat29.76
library_construction_protocolNot reported
library_gen_protocolNot reported
library_layoutSINGLE
library_max_fragment_sizeNot reported
library_min_fragment_sizeNot reported
library_namesaliva_conc_18
library_pcr_isolation_protocolNot reported
library_prep_dateNot reported
library_prep_date_formatNot reported
library_prep_latitudeNot reported
library_prep_locationNot reported
library_prep_longitudeNot reported
library_selectionPCR
library_sourceGENOMIC
library_strategyAMPLICON
local_environmental_contextNot reported
location29.76 N 95.37 W
location_end29.76 N 95.37 W
location_start29.76 N 95.37 W
lon-95.37
marine_regionNot reported
mating_typeNot reported
ncbi_reporting_standardMetagenome or environmental
nominal_lengthNot reported
nominal_sdevNot reported
pcr_isolation_protocolNot reported
phNot reported
project_nameNot reported
protocol_labelNot reported
read_count184000
read_strandNot reported
restriction_enzymeNot reported
restriction_enzyme_target_sequenceNot reported
restriction_siteNot reported
rna_integrity_numNot reported
rna_prep_3_protocolNot reported
rna_prep_5_protocolNot reported
rna_purity_230_ratioNot reported
rna_purity_280_ratioNot reported
rt_prep_protocolNot reported
run_aliassaliva_conc_18.fq
run_dateNot reported
salinityNot reported
sample_accessionSAMN17774963
sample_aliasfootball19_saliva_conc_18
sample_capture_statusNot reported
sample_collectionNot reported
sample_descriptionMetagenome or environmental sample from human oral metagenome
sample_materialNot reported
sample_prep_intervalNot reported
sample_prep_interval_unitsNot reported
sample_storageNot reported
sample_storage_processingNot reported
sample_titleMetagenome or environmental sample from human oral metagenome
sampling_campaignNot reported
sampling_platformNot reported
sampling_siteNot reported
scientific_namehuman oral metagenome
secondary_projectNot reported
secondary_sample_accessionSRS8230804
secondary_study_accessionSRP305684
sequencing_dateNot reported
sequencing_date_formatNot reported
sequencing_locationNot reported
sequencing_longitudeNot reported
sequencing_methodNot reported
sequencing_primer_catalogNot reported
sequencing_primer_lotNot reported
sequencing_primer_providerNot reported
serotypeNot reported
serovarNot reported
sexNot reported
specimen_voucherNot reported
sra_asperaNot reported
sra_bytesNot reported
sra_file_roleNot reported
sra_ftpNot reported
sra_galaxyNot reported
sra_md5Not reported
statuspublic
strainNot reported
study_accessionPRJNA699316
study_aliasPRJNA699316
study_titleGut and saliva microbiome in collegiate football athletes
sub_speciesNot reported
sub_strainNot reported
submission_accessionSRA1194482
submission_toolNot reported
submitted_asperaNot reported
submitted_bytesNot reported
submitted_file_roleNot reported
submitted_formatNot reported
submitted_ftpNot reported
submitted_galaxyNot reported
submitted_host_sexNot reported
submitted_md5Not reported
submitted_read_typeNot reported
surveillance_targetNot reported
tagenv_geo;env_geo:terrestrial
target_geneNot reported
tax_id447426
tax_lineage1;2787823;12908;408169;410656;447426
taxonomic_classificationNot reported
taxonomic_identity_markerNot reported
temperatureNot reported
tissue_libNot reported
tissue_typeNot reported
transposase_protocolNot reported
varietyNot reported
BioSample record and attributes
aliasfootball19_saliva_conc_18
titleMetagenome or environmental sample from human oral metagenome
descriptionNot reported
collection_date2019-11
INSDC secondary accessionSRS8230804
NCBI submission packageMetagenome.environmental.1.0
player_id18
organismhuman oral metagenome
post-concussionyes
time_pointbetween mid-post
hostHomo sapiens
geo_loc_nameUSA: Texas
samp_mat_processNorgen Saliva DNA Isolation Reagent Kit, MinION nanopore sequencer
lat_lon29.76 N 95.37 W
isolation_sourcesaliva
BioSampleModelMetagenome or environmental
ENA-FIRST-PUBLIC2021-02-03
ENA-LAST-UPDATE2021-02-28
Curated cohort annotations and QC
runSRR13679659
sampleSAMN17774963
studyPRJNA699316
titleMetagenome or environmental sample from human oral metagenome
aliasfootball19_saliva_conc_18
sitesaliva
subject18
phenotypeNot reported
sexNot reported
smokingNot reported
periodontitisNot reported
time_pointbetween mid-post
negative_controlFalse
lab_modelFalse
statecomplete
reads_input30000
species_reported69
human_primaryTrue

Model-based taxonomy; research use. This report does not provide a diagnosis or a validated health score.