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archAIons · MetaBiome Project · Oral · Public research data

SRR13679649

Metagenome or environmental sample from human oral metagenome

BioSample: SAMN17774964 · Study: PRJNA699316 · Body site: saliva

Interactive report · NCBI SRA · ENA

86Reported species
14Reported genera

Taxonomic abundance

Relative abundance is normalized among reported taxa. No reported taxa does not establish absence of organisms.

SpeciesGenusPhylumAbundance
Streptococcus salivariusStreptococcusBacillota12.88713%
Veillonella parvulaVeillonellaBacillota10.17601%
Streptococcus anginosusStreptococcusBacillota6.31901%
Haemophilus parainfluenzaeHaemophilusPseudomonadota5.40103%
Veillonella disparVeillonellaBacillota4.43951%
Streptococcus downeiStreptococcusBacillota4.00751%
Streptococcus pneumoniaeStreptococcusBacillota3.33906%
Neisseria meningitidisNeisseriaPseudomonadota3.19373%
Streptococcus gordoniiStreptococcusBacillota3.08596%
Veillonella rodentiumVeillonellaBacillota2.88680%
Streptococcus sanguinisStreptococcusBacillota2.71895%
Streptococcus mitisStreptococcusBacillota2.26115%
Haemophilus influenzaeHaemophilusPseudomonadota2.15855%
Neisseria gonorrhoeaeNeisseriaPseudomonadota2.08952%
Neisseria elongataNeisseriaPseudomonadota2.02153%
Neisseria dentiaeNeisseriaPseudomonadota1.70086%
Gemella palaticanisGemellaBacillota1.60082%
Streptococcus pyogenesStreptococcusBacillota1.51835%
Gemella haemolysansGemellaBacillota1.45503%
Neisseria flavescensNeisseriaPseudomonadota1.35242%
Neisseria lactamicaNeisseriaPseudomonadota1.32565%
Campylobacter concisusCampylobacterPseudomonadota1.08378%
Streptococcus oralisStreptococcusBacillota1.04840%
Megasphaera elsdeniiMegasphaeraBacillota1.04773%
Granulicatella balaenopteraeGranulicatellaBacillota1.02496%
Neisseria mucosaNeisseriaPseudomonadota1.00878%
Veillonella infantiumVeillonellaBacillota0.83441%
Neisseria polysacchareaNeisseriaPseudomonadota0.73600%
Neisseria animalorisNeisseriaPseudomonadota0.71273%
Campylobacter curvusCampylobacterPseudomonadota0.70159%
Haemophilus aegyptiusHaemophilusPseudomonadota0.67200%
Neisseria subflavaNeisseriaPseudomonadota0.63209%
Gemella parahaemolysansGemellaBacillota0.61790%
Neisseria canisNeisseriaPseudomonadota0.59002%
Campylobacter sputorumCampylobacterPseudomonadota0.57505%
Granulicatella adiacensGranulicatellaBacillota0.54772%
Streptococcus himalayensisStreptococcusBacillota0.54067%
Veillonella seminalisVeillonellaBacillota0.53500%
Streptococcus periodonticumStreptococcusBacillota0.46345%
Haemophilus pittmaniaeHaemophilusPseudomonadota0.45699%
Neisseria zoodegmatisNeisseriaPseudomonadota0.45518%
Granulicatella elegansGranulicatellaBacillota0.44303%
Streptococcus parasanguinisStreptococcusBacillota0.43637%
Streptococcus marmotaeStreptococcusBacillota0.43032%
Megasphaera paucivoransMegasphaeraBacillota0.41669%
Neisseria animalisNeisseriaPseudomonadota0.40916%
Fusobacterium nucleatumFusobacteriumFusobacteriota0.36933%
Gemella morbillorumGemellaBacillota0.35719%
Megasphaera sueciensisMegasphaeraBacillota0.34230%
Prevotella melaninogenicaPrevotellaBacteroidota0.31390%
Streptococcus cristatusStreptococcusBacillota0.30197%
Neisseria macacaeNeisseriaPseudomonadota0.29932%
Gemella asaccharolyticaGemellaBacillota0.29886%
Abiotrophia defectivaAbiotrophiaBacillota0.27747%
Megasphaera stantoniiMegasphaeraBacillota0.27189%
Streptococcus pluranimaliumStreptococcusBacillota0.26602%
Streptococcus intermediusStreptococcusBacillota0.26153%
Neisseria iguanaeNeisseriaPseudomonadota0.25909%
Haemophilus haemoglobinophilusHaemophilusPseudomonadota0.25336%
Megasphaera hexanoicaMegasphaeraBacillota0.25032%
Prevotella intermediaPrevotellaBacteroidota0.20457%
Haemophilus haemolyticusHaemophilusPseudomonadota0.19705%
Prevotella jejuniPrevotellaBacteroidota0.19438%
Streptococcus alactolyticusStreptococcusBacillota0.18904%
Campylobacter hyointestinalisCampylobacterPseudomonadota0.16029%
Gemella cuniculiGemellaBacillota0.15838%
Veillonella rattiVeillonellaBacillota0.15694%
Streptococcus sinensisStreptococcusBacillota0.15415%
Streptococcus milleriStreptococcusBacillota0.14819%
Fusobacterium periodonticumFusobacteriumFusobacteriota0.14168%
Neisseria siccaNeisseriaPseudomonadota0.13748%
Streptococcus infantisStreptococcusBacillota0.12582%
Veillonella cricetiVeillonellaBacillota0.12198%
Neisseria chenwenguiiNeisseriaPseudomonadota0.12058%
Streptococcus ruminantiumStreptococcusBacillota0.12046%
Streptococcus constellatusStreptococcusBacillota0.11789%
Selenomonas sputigenaSelenomonasBacillota0.11621%
Selenomonas ruminantiumSelenomonasBacillota0.11589%
Porphyromonas gingivalisPorphyromonasBacteroidota0.11165%
Gemella taiwanensisGemellaBacillota0.11094%
Haemophilus paraphrohaemolyticusHaemophilusPseudomonadota0.11085%
Streptococcus acidominimusStreptococcusBacillota0.10917%
Morococcus cerebrosusMorococcusPseudomonadota0.10706%
Streptococcus koreensisStreptococcusBacillota0.10124%
Streptococcus sobrinusStreptococcusBacillota0.09679%
Haemophilus parahaemolyticusHaemophilusPseudomonadota0.09014%

Reference-based 16S phylogeny

86 of 86 reported species have an eligible reference. MAFFT alignment and FastTree GTR+CAT tree, pruned to this sample. This is not a phylogeny inferred from sample reads or ASVs. Root placement is arbitrary; branch lengths are substitutions per site.

Streptococcus periodonticum0.46%Streptococcus anginosus6.32%Streptococcus milleri0.15%Streptococcus constellatus0.12%Streptococcus intermedius0.26%Streptococcus cristatus0.30%Streptococcus sinensis0.15%Streptococcus parasanguinis0.44%Streptococcus infantis0.13%Streptococcus oralis1.05%Streptococcus mitis2.26%Streptococcus pneumoniae3.34%Streptococcus koreensis0.10%Streptococcus himalayensis0.54%Streptococcus marmotae0.43%Streptococcus gordonii3.09%Streptococcus sanguinis2.72%Streptococcus acidominimus0.11%Streptococcus alactolyticus0.19%Streptococcus pyogenes1.52%Streptococcus ruminantium0.12%Abiotrophia defectiva0.28%Granulicatella balaenopterae1.02%Granulicatella adiacens0.55%Granulicatella elegans0.44%Gemella asaccharolytica0.30%Gemella palaticanis1.60%Gemella cuniculi0.16%Gemella parahaemolysans0.62%Gemella taiwanensis0.11%Gemella haemolysans1.46%Gemella morbillorum0.36%Veillonella criceti0.12%Veillonella ratti0.16%Veillonella seminalis0.54%Veillonella dispar4.44%Veillonella infantium0.83%Veillonella parvula10.18%Veillonella rodentium2.89%Megasphaera stantonii0.27%Megasphaera paucivorans0.42%Megasphaera sueciensis0.34%Megasphaera elsdenii1.05%Megasphaera hexanoica0.25%Selenomonas ruminantium0.12%Selenomonas sputigena0.12%Fusobacterium periodonticum0.14%Fusobacterium nucleatum0.37%Campylobacter sputorum0.58%Campylobacter curvus0.70%Campylobacter concisus1.08%Campylobacter hyointestinalis0.16%Neisseria canis0.59%Neisseria chenwenguii0.12%Neisseria zoodegmatis0.46%Neisseria animaloris0.71%Neisseria dentiae1.70%Neisseria animalis0.41%Neisseria subflava0.63%Morococcus cerebrosus0.11%Neisseria mucosa1.01%Neisseria macacae0.30%Neisseria sicca0.14%Neisseria lactamica1.33%Neisseria polysaccharea0.74%Neisseria meningitidis3.19%Neisseria gonorrhoeae2.09%Neisseria iguanae0.26%Neisseria flavescens1.35%Neisseria elongata2.02%Haemophilus parainfluenzae5.40%Haemophilus parahaemolyticus0.09%Haemophilus paraphrohaemolyticus0.11%Haemophilus haemoglobinophilus0.25%Haemophilus haemolyticus0.20%Haemophilus aegyptius0.67%Haemophilus influenzae2.16%Haemophilus pittmaniae0.46%Porphyromonas gingivalis0.11%Prevotella jejuni0.19%Prevotella melaninogenica0.31%Prevotella intermedia0.20%Streptococcus sobrinus0.10%Streptococcus downei4.01%Streptococcus pluranimalium0.27%Streptococcus salivarius12.89%Branch length scale: 1.0631 substitutions/site across the 500-unit horizontal axis. Root arbitrary.

Public raw read files

Files contain the original full run. Downloads are hosted by ENA.

FileType / bytesMD5
SRR13679649_1.fastq.gzFASTQ / 28927389376ca8f58f2cd3902e054f9ab2d62b5d1

Analysis provenance

marker16S
pipelineArchaions v18.8
read_samplingFirst available reads, not random subsampling
input_sha2567d5015a1d675152bbba48784d4d48bec59c9ab64099996e1d64628ec5b445ea9
median_read_length1585.0
reads_in_length_window28553
length_window_bp[1200, 2000]
quality_scoresUnavailable in fetched FASTA; no measured quality filtering
classifier_stats{"marker": "16s", "pipeline": "v18.8", "gpu": "RTX-PRO-6000", "input_path": "volume-fastq-gz", "reads_in": 30000, "reads_skipped": 6567, "elapsed_seconds": 2.614009573000004, "reads_submitted": 30000, "reads_after_filter": 30000, "adapter_scan": {"sampled": 200, "matched": 35, "fraction": 0.175, "adapters_found": {"1492R_rc": 20, "27F": 15}, "should_trim": true}, "porechop_ran": false, "porechop_reason": "fast-path: no adapter trimming required"}

Full public metadata

All retrieved values are included. Blank source values are displayed as “Not reported”. Expand the sections below to inspect the full record.

ENA run fields (195)
run_accessionSRR13679649
ageNot reported
alignedNot reported
altitudeNot reported
assembly_qualityNot reported
assembly_softwareNot reported
bam_asperaNot reported
bam_bytesNot reported
bam_file_roleNot reported
bam_ftpNot reported
bam_galaxyNot reported
bam_md5Not reported
base_count289666349
binning_softwareNot reported
bio_materialNot reported
bisulfite_protocolNot reported
broad_scale_environmental_contextNot reported
broker_nameNot reported
cage_protocolNot reported
cell_lineNot reported
cell_typeNot reported
center_nameSUB9029329
checklistNot reported
chip_ab_providerNot reported
chip_protocolNot reported
chip_targetNot reported
collected_byNot reported
collection_date2019-12
collection_date_end2019-12-31
collection_date_start2019-12-01
completeness_scoreNot reported
contamination_scoreNot reported
control_experimentNot reported
countryUSA: Texas
cultivarNot reported
culture_collectionNot reported
datahubdcc_metagenome
depthNot reported
descriptionMinION sequencing: 16s RNA of human oral metagenome: conc 23
dev_stageNot reported
diseaseNot reported
dnase_protocolNot reported
ecotypeNot reported
elevationNot reported
environment_biomeNot reported
environment_featureNot reported
environment_materialNot reported
environmental_mediumNot reported
environmental_sampleNot reported
experiment_accessionSRX10069054
experiment_aliassaliva_conc_23
experiment_targetNot reported
experiment_titleMinION sequencing: 16s RNA of human oral metagenome: conc 23
experimental_factorNot reported
experimental_protocolNot reported
extraction_protocolNot reported
faang_library_selectionNot reported
fastq_asperafasp.sra.ebi.ac.uk:/vol1/fastq/SRR136/049/SRR13679649/SRR13679649_1.fastq.gz
fastq_bytes289273893
fastq_file_roleGENERATED_FILE
fastq_ftpftp.sra.ebi.ac.uk/vol1/fastq/SRR136/049/SRR13679649/SRR13679649_1.fastq.gz
fastq_galaxyftp.sra.ebi.ac.uk/vol1/fastq/SRR136/049/SRR13679649/SRR13679649_1.fastq.gz
fastq_md576ca8f58f2cd3902e054f9ab2d62b5d1
file_locationNot reported
first_created2022-03-03
first_public2022-03-03
germlineNot reported
hi_c_protocolNot reported
hostHomo sapiens
host_body_siteNot reported
host_genotypeNot reported
host_gravidityNot reported
host_growth_conditionsNot reported
host_phenotypeNot reported
host_scientific_nameHomo sapiens
host_sexNot reported
host_statusNot reported
host_tax_id9606
identified_byNot reported
instrument_modelMinION
instrument_platformOXFORD_NANOPORE
investigation_typeNot reported
isolateNot reported
isolation_sourcesaliva
last_updated2022-03-03
lat29.76
library_construction_protocolNot reported
library_gen_protocolNot reported
library_layoutSINGLE
library_max_fragment_sizeNot reported
library_min_fragment_sizeNot reported
library_namesaliva_conc_23
library_pcr_isolation_protocolNot reported
library_prep_dateNot reported
library_prep_date_formatNot reported
library_prep_latitudeNot reported
library_prep_locationNot reported
library_prep_longitudeNot reported
library_selectionPCR
library_sourceGENOMIC
library_strategyAMPLICON
local_environmental_contextNot reported
location29.76 N 95.37 W
location_end29.76 N 95.37 W
location_start29.76 N 95.37 W
lon-95.37
marine_regionNot reported
mating_typeNot reported
ncbi_reporting_standardMetagenome or environmental
nominal_lengthNot reported
nominal_sdevNot reported
pcr_isolation_protocolNot reported
phNot reported
project_nameNot reported
protocol_labelNot reported
read_count188000
read_strandNot reported
restriction_enzymeNot reported
restriction_enzyme_target_sequenceNot reported
restriction_siteNot reported
rna_integrity_numNot reported
rna_prep_3_protocolNot reported
rna_prep_5_protocolNot reported
rna_purity_230_ratioNot reported
rna_purity_280_ratioNot reported
rt_prep_protocolNot reported
run_aliassaliva_conc_23.fq
run_dateNot reported
salinityNot reported
sample_accessionSAMN17774964
sample_aliasfootball19_saliva_conc_23
sample_capture_statusNot reported
sample_collectionNot reported
sample_descriptionMetagenome or environmental sample from human oral metagenome
sample_materialNot reported
sample_prep_intervalNot reported
sample_prep_interval_unitsNot reported
sample_storageNot reported
sample_storage_processingNot reported
sample_titleMetagenome or environmental sample from human oral metagenome
sampling_campaignNot reported
sampling_platformNot reported
sampling_siteNot reported
scientific_namehuman oral metagenome
secondary_projectNot reported
secondary_sample_accessionSRS8230815
secondary_study_accessionSRP305684
sequencing_dateNot reported
sequencing_date_formatNot reported
sequencing_locationNot reported
sequencing_longitudeNot reported
sequencing_methodNot reported
sequencing_primer_catalogNot reported
sequencing_primer_lotNot reported
sequencing_primer_providerNot reported
serotypeNot reported
serovarNot reported
sexNot reported
specimen_voucherNot reported
sra_asperaNot reported
sra_bytesNot reported
sra_file_roleNot reported
sra_ftpNot reported
sra_galaxyNot reported
sra_md5Not reported
statuspublic
strainNot reported
study_accessionPRJNA699316
study_aliasPRJNA699316
study_titleGut and saliva microbiome in collegiate football athletes
sub_speciesNot reported
sub_strainNot reported
submission_accessionSRA1194482
submission_toolNot reported
submitted_asperaNot reported
submitted_bytesNot reported
submitted_file_roleNot reported
submitted_formatNot reported
submitted_ftpNot reported
submitted_galaxyNot reported
submitted_host_sexNot reported
submitted_md5Not reported
submitted_read_typeNot reported
surveillance_targetNot reported
tagenv_geo;env_geo:terrestrial
target_geneNot reported
tax_id447426
tax_lineage1;2787823;12908;408169;410656;447426
taxonomic_classificationNot reported
taxonomic_identity_markerNot reported
temperatureNot reported
tissue_libNot reported
tissue_typeNot reported
transposase_protocolNot reported
varietyNot reported
BioSample record and attributes
aliasfootball19_saliva_conc_23
titleMetagenome or environmental sample from human oral metagenome
descriptionNot reported
collection_date2019-12
INSDC secondary accessionSRS8230815
NCBI submission packageMetagenome.environmental.1.0
player_id23
organismhuman oral metagenome
post-concussionyes
time_pointpost-season
hostHomo sapiens
geo_loc_nameUSA: Texas
samp_mat_processNorgen Saliva DNA Isolation Reagent Kit, MinION nanopore sequencer
lat_lon29.76 N 95.37 W
isolation_sourcesaliva
BioSampleModelMetagenome or environmental
ENA-FIRST-PUBLIC2021-02-03
ENA-LAST-UPDATE2021-02-28
Curated cohort annotations and QC
runSRR13679649
sampleSAMN17774964
studyPRJNA699316
titleMetagenome or environmental sample from human oral metagenome
aliasfootball19_saliva_conc_23
sitesaliva
subject23
phenotypeNot reported
sexNot reported
smokingNot reported
periodontitisNot reported
time_pointpost-season
negative_controlFalse
lab_modelFalse
statecomplete
reads_input30000
species_reported86
human_primaryTrue

Model-based taxonomy; research use. This report does not provide a diagnosis or a validated health score.