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archAIons · MetaBiome Project · Oral · Public research data

SRR12522767

Metagenome or environmental sample from human oral metagenome

BioSample: SAMN15887564 · Study: PRJNA658726 · Body site: Subgingival

Interactive report · NCBI SRA · ENA

41Reported species
10Reported genera

Taxonomic abundance

Relative abundance is normalized among reported taxa. No reported taxa does not establish absence of organisms.

SpeciesGenusPhylumAbundance
Streptococcus mitisStreptococcusBacillota28.51697%
Streptococcus downeiStreptococcusBacillota9.90623%
Neisseria flavescensNeisseriaPseudomonadota6.80169%
Streptococcus salivariusStreptococcusBacillota5.81378%
Streptococcus pneumoniaeStreptococcusBacillota5.66923%
Haemophilus parainfluenzaeHaemophilusPseudomonadota5.18127%
Neisseria macacaeNeisseriaPseudomonadota3.86367%
Veillonella parvulaVeillonellaBacillota3.25158%
Fusobacterium nucleatumFusobacteriumFusobacteriota2.43399%
Neisseria mucosaNeisseriaPseudomonadota2.26383%
Haemophilus haemolyticusHaemophilusPseudomonadota2.26358%
Streptococcus infantisStreptococcusBacillota1.93854%
Capnocytophaga canisCapnocytophagaBacteroidota1.42772%
Streptococcus oralisStreptococcusBacillota1.41227%
Granulicatella adiacensGranulicatellaBacillota1.33671%
Campylobacter gracilisCampylobacterPseudomonadota1.20314%
Streptococcus pyogenesStreptococcusBacillota1.16186%
Fusobacterium perfoetensFusobacteriumFusobacteriota1.04543%
Gemella palaticanisGemellaBacillota1.01127%
Neisseria gonorrhoeaeNeisseriaPseudomonadota0.96379%
Campylobacter hyointestinalisCampylobacterPseudomonadota0.93699%
Streptococcus alactolyticusStreptococcusBacillota0.91544%
Streptococcus sanguinisStreptococcusBacillota0.90128%
Streptococcus cristatusStreptococcusBacillota0.79747%
Neisseria dentiaeNeisseriaPseudomonadota0.78684%
Campylobacter showaeCampylobacterPseudomonadota0.76980%
Streptococcus gordoniiStreptococcusBacillota0.74919%
Campylobacter sputorumCampylobacterPseudomonadota0.70474%
Gemella haemolysansGemellaBacillota0.68414%
Neisseria meningitidisNeisseriaPseudomonadota0.58156%
Streptococcus intermediusStreptococcusBacillota0.56841%
Haemophilus aegyptiusHaemophilusPseudomonadota0.49777%
Streptococcus australisStreptococcusBacillota0.49702%
Veillonella infantiumVeillonellaBacillota0.46645%
Haemophilus parahaemolyticusHaemophilusPseudomonadota0.46011%
Streptococcus periodonticumStreptococcusBacillota0.41647%
Neisseria canisNeisseriaPseudomonadota0.41381%
Streptococcus macacaeStreptococcusBacillota0.40255%
Streptococcus marmotaeStreptococcusBacillota0.35985%
Streptococcus parasanguinisStreptococcusBacillota0.35585%
Leptotrichia hongkongensisLeptotrichiaFusobacteriota0.26770%

Reference-based 16S phylogeny

40 of 41 reported species have an eligible reference. MAFFT alignment and FastTree GTR+CAT tree, pruned to this sample. This is not a phylogeny inferred from sample reads or ASVs. Root placement is arbitrary; branch lengths are substitutions per site.

Streptococcus periodonticum0.42%Streptococcus intermedius0.57%Streptococcus cristatus0.80%Streptococcus parasanguinis0.36%Streptococcus infantis1.94%Streptococcus oralis1.41%Streptococcus mitis28.52%Streptococcus pneumoniae5.67%Streptococcus australis0.50%Streptococcus marmotae0.36%Streptococcus gordonii0.75%Streptococcus sanguinis0.90%Streptococcus alactolyticus0.92%Streptococcus pyogenes1.16%Granulicatella adiacens1.34%Gemella palaticanis1.01%Gemella haemolysans0.68%Veillonella infantium0.47%Veillonella parvula3.25%Fusobacterium perfoetens1.05%Fusobacterium nucleatum2.43%Leptotrichia hongkongensis0.27%Campylobacter sputorum0.70%Campylobacter gracilis1.20%Campylobacter showae0.77%Campylobacter hyointestinalis0.94%Neisseria canis0.41%Neisseria dentiae0.79%Neisseria mucosa2.26%Neisseria macacae3.86%Neisseria meningitidis0.58%Neisseria gonorrhoeae0.96%Neisseria flavescens6.80%Haemophilus parainfluenzae5.18%Haemophilus parahaemolyticus0.46%Haemophilus haemolyticus2.26%Haemophilus aegyptius0.50%Streptococcus macacae0.40%Streptococcus downei9.91%Streptococcus salivarius5.81%Branch length scale: 0.7215 substitutions/site across the 500-unit horizontal axis. Root arbitrary.

Public raw read files

Files contain the original full run. Downloads are hosted by ENA.

FileType / bytesMD5
SRR12522767_1.fastq.gzFASTQ / 1715473879df6e36c6819c58ea31198853ee426ec

Analysis provenance

marker16S
pipelineArchaions v18.8
read_samplingFirst available reads, not random subsampling
input_sha256985213a17626cc721d115e211f1edfe1d3f931552bf4066fa771d9489d2bb417
median_read_length1423.0
reads_in_length_window8839
length_window_bp[1200, 2000]
quality_scoresUnavailable in fetched FASTA; no measured quality filtering
classifier_stats{"marker": "16s", "pipeline": "v18.8", "gpu": "RTX-PRO-6000", "input_path": "volume-fastq-gz", "reads_in": 10000, "reads_skipped": 3182, "elapsed_seconds": 1.2356572409999984, "reads_submitted": 10000, "reads_after_filter": 10000, "adapter_scan": {"sampled": 200, "matched": 10, "fraction": 0.05, "adapters_found": {"BC_FLANK": 10}, "should_trim": true}, "porechop_ran": false, "porechop_reason": "fast-path: no adapter trimming required"}

Full public metadata

All retrieved values are included. Blank source values are displayed as “Not reported”. Expand the sections below to inspect the full record.

ENA run fields (195)
run_accessionSRR12522767
ageNot reported
alignedNot reported
altitudeNot reported
assembly_qualityNot reported
assembly_softwareNot reported
bam_asperaNot reported
bam_bytesNot reported
bam_file_roleNot reported
bam_ftpNot reported
bam_galaxyNot reported
bam_md5Not reported
base_count166059037
binning_softwareNot reported
bio_materialNot reported
bisulfite_protocolNot reported
broad_scale_environmental_contextNot reported
broker_nameNot reported
cage_protocolNot reported
cell_lineNot reported
cell_typeNot reported
center_nameSUB7951364
checklistNot reported
chip_ab_providerNot reported
chip_protocolNot reported
chip_targetNot reported
collected_byNot reported
collection_datenot applicable
collection_date_endNot reported
collection_date_startNot reported
completeness_scoreNot reported
contamination_scoreNot reported
control_experimentNot reported
countryUnited Arab Emirates
cultivarNot reported
culture_collectionNot reported
datahubdcc_metagenome
depthNot reported
descriptionMinION sequencing: metagenomic
dev_stageNot reported
diseaseNot reported
dnase_protocolNot reported
ecotypeNot reported
elevationNot reported
environment_biomeNot reported
environment_featureNot reported
environment_materialNot reported
environmental_mediumNot reported
environmental_sampleNot reported
experiment_accessionSRX9012847
experiment_aliasC4-P
experiment_targetNot reported
experiment_titleMinION sequencing: metagenomic
experimental_factorNot reported
experimental_protocolNot reported
extraction_protocolNot reported
faang_library_selectionNot reported
fastq_asperafasp.sra.ebi.ac.uk:/vol1/fastq/SRR125/067/SRR12522767/SRR12522767_1.fastq.gz
fastq_bytes171547387
fastq_file_roleGENERATED_FILE
fastq_ftpftp.sra.ebi.ac.uk/vol1/fastq/SRR125/067/SRR12522767/SRR12522767_1.fastq.gz
fastq_galaxyftp.sra.ebi.ac.uk/vol1/fastq/SRR125/067/SRR12522767/SRR12522767_1.fastq.gz
fastq_md59df6e36c6819c58ea31198853ee426ec
file_locationNot reported
first_created2021-10-01
first_public2021-10-01
germlineNot reported
hi_c_protocolNot reported
hostHomo sapiens
host_body_siteNot reported
host_genotypeNot reported
host_gravidityNot reported
host_growth_conditionsNot reported
host_phenotypeNot reported
host_scientific_nameHomo sapiens
host_sexNot reported
host_statusNot reported
host_tax_id9606
identified_byNot reported
instrument_modelMinION
instrument_platformOXFORD_NANOPORE
investigation_typeNot reported
isolatemixed1
isolation_sourceoral
last_updated2021-10-01
latNot reported
library_construction_protocolNot reported
library_gen_protocolNot reported
library_layoutSINGLE
library_max_fragment_sizeNot reported
library_min_fragment_sizeNot reported
library_nameC4-P
library_pcr_isolation_protocolNot reported
library_prep_dateNot reported
library_prep_date_formatNot reported
library_prep_latitudeNot reported
library_prep_locationNot reported
library_prep_longitudeNot reported
library_selectionPCR
library_sourceGENOMIC
library_strategyAMPLICON
local_environmental_contextNot reported
locationNot reported
location_endNot reported
location_startNot reported
lonNot reported
marine_regionNot reported
mating_typeNot reported
ncbi_reporting_standardMetagenome or environmental
nominal_lengthNot reported
nominal_sdevNot reported
pcr_isolation_protocolNot reported
phNot reported
project_nameNot reported
protocol_labelNot reported
read_count121335
read_strandNot reported
restriction_enzymeNot reported
restriction_enzyme_target_sequenceNot reported
restriction_siteNot reported
rna_integrity_numNot reported
rna_prep_3_protocolNot reported
rna_prep_5_protocolNot reported
rna_purity_230_ratioNot reported
rna_purity_280_ratioNot reported
rt_prep_protocolNot reported
run_aliasC4.fastq
run_dateNot reported
salinityNot reported
sample_accessionSAMN15887564
sample_aliasC4
sample_capture_statusNot reported
sample_collectionNot reported
sample_descriptionMetagenome or environmental sample from human oral metagenome
sample_materialNot reported
sample_prep_intervalNot reported
sample_prep_interval_unitsNot reported
sample_storageNot reported
sample_storage_processingNot reported
sample_titleMetagenome or environmental sample from human oral metagenome
sampling_campaignNot reported
sampling_platformNot reported
sampling_siteNot reported
scientific_namehuman oral metagenome
secondary_projectNot reported
secondary_sample_accessionSRS7263859
secondary_study_accessionSRP278853
sequencing_dateNot reported
sequencing_date_formatNot reported
sequencing_locationNot reported
sequencing_longitudeNot reported
sequencing_methodNot reported
sequencing_primer_catalogNot reported
sequencing_primer_lotNot reported
sequencing_primer_providerNot reported
serotypeNot reported
serovarNot reported
sexNot reported
specimen_voucherNot reported
sra_asperaNot reported
sra_bytesNot reported
sra_file_roleNot reported
sra_ftpNot reported
sra_galaxyNot reported
sra_md5Not reported
statuspublic
strainmixed
study_accessionPRJNA658726
study_aliasPRJNA658726
study_titleThe impact of smoking different tobacco types on the subgingival microbiome and periodontal health: a pilot study
sub_speciesNot reported
sub_strainNot reported
submission_accessionSRA1117712
submission_toolNot reported
submitted_asperaNot reported
submitted_bytesNot reported
submitted_file_roleNot reported
submitted_formatNot reported
submitted_ftpNot reported
submitted_galaxyNot reported
submitted_host_sexNot reported
submitted_md5Not reported
submitted_read_typeNot reported
surveillance_targetNot reported
tagNot reported
target_geneNot reported
tax_id447426
tax_lineage1;2787823;12908;408169;410656;447426
taxonomic_classificationNot reported
taxonomic_identity_markerNot reported
temperatureNot reported
tissue_libNot reported
tissue_typeNot reported
transposase_protocolNot reported
varietyNot reported
BioSample record and attributes
aliasC4
titleMetagenome or environmental sample from human oral metagenome
descriptionNot reported
strainmixed
collection_datenot applicable
INSDC secondary accessionSRS7263859
aliquotcig1
organismhuman oral metagenome
sample_typeSubgingival
isolatemixed1
NCBI submission packageMetagenome.environmental.1.0
periodontitisno-mild
smokingS-Cigarettes
hostHomo sapiens
geo_loc_nameUnited Arab Emirates
lat_lonmissing
isolation_sourceoral
BioSampleModelMetagenome or environmental
ENA-FIRST-PUBLIC2021-09-30
ENA-LAST-UPDATE2021-09-30
Curated cohort annotations and QC
runSRR12522767
sampleSAMN15887564
studyPRJNA658726
titleMetagenome or environmental sample from human oral metagenome
aliasC4
siteSubgingival
subjectNot reported
phenotypeNot reported
sexNot reported
smokingS-Cigarettes
periodontitisno-mild
time_pointNot reported
negative_controlFalse
lab_modelFalse
statecomplete
reads_input10000
species_reported41
human_primaryTrue

Model-based taxonomy; research use. This report does not provide a diagnosis or a validated health score.