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SRR12522762

Metagenome or environmental sample from human oral metagenome

BioSample: SAMN15887577 · Study: PRJNA658726 · Body site: Subgingival

Interactive report · NCBI SRA · ENA

51Reported species
15Reported genera

Taxonomic abundance

Relative abundance is normalized among reported taxa. No reported taxa does not establish absence of organisms.

SpeciesGenusPhylumAbundance
Streptococcus mitisStreptococcusBacillota8.77496%
Selenomonas flueggeiSelenomonasBacillota8.34716%
Veillonella parvulaVeillonellaBacillota7.88769%
Fusobacterium nucleatumFusobacteriumFusobacteriota6.38306%
Neisseria flavescensNeisseriaPseudomonadota5.12095%
Streptococcus downeiStreptococcusBacillota4.78517%
Campylobacter gracilisCampylobacterPseudomonadota4.06758%
Campylobacter showaeCampylobacterPseudomonadota3.89401%
Campylobacter hyointestinalisCampylobacterPseudomonadota3.75478%
Fusobacterium perfoetensFusobacteriumFusobacteriota3.65266%
Capnocytophaga canisCapnocytophagaBacteroidota3.54607%
Neisseria macacaeNeisseriaPseudomonadota2.90017%
Campylobacter sputorumCampylobacterPseudomonadota2.88849%
Leptotrichia hongkongensisLeptotrichiaFusobacteriota2.07736%
Streptococcus salivariusStreptococcusBacillota2.02939%
Prevotella ruminicolaPrevotellaBacteroidota1.98215%
Dialister invisusDialisterBacillota1.93979%
Selenomonas dianaeSelenomonasBacillota1.66496%
Selenomonas sputigenaSelenomonasBacillota1.62682%
Fusobacterium russiiFusobacteriumFusobacteriota1.34558%
Lachnoanaerobaculum saburreumLachnoanaerobaculumBacillota1.27330%
Streptococcus pneumoniaeStreptococcusBacillota1.22284%
Aggregatibacter segnisAggregatibacterPseudomonadota1.08514%
Streptococcus cristatusStreptococcusBacillota1.06681%
Eubacterium ramulusEubacteriumBacillota1.05626%
Lachnoanaerobaculum oraleLachnoanaerobaculumBacillota0.99554%
Aggregatibacter aphrophilusAggregatibacterPseudomonadota0.96155%
Selenomonas ruminantiumSelenomonasBacillota0.94944%
Campylobacter concisusCampylobacterPseudomonadota0.91438%
Prevotella orisPrevotellaBacteroidota0.89370%
Gemella palaticanisGemellaBacillota0.77322%
Selenomonas noxiaSelenomonasBacillota0.73657%
Neisseria gonorrhoeaeNeisseriaPseudomonadota0.69063%
Streptococcus infantisStreptococcusBacillota0.64714%
Filifactor alocisFilifactorBacillota0.61793%
Lachnoanaerobaculum umeaenseLachnoanaerobaculumBacillota0.61167%
Capnocytophaga granulosaCapnocytophagaBacteroidota0.60632%
Streptococcus sanguinisStreptococcusBacillota0.60539%
Neisseria dentiaeNeisseriaPseudomonadota0.58303%
Veillonella infantiumVeillonellaBacillota0.57272%
Neisseria meningitidisNeisseriaPseudomonadota0.53640%
Filifactor villosusFilifactorBacillota0.52411%
Neisseria elongataNeisseriaPseudomonadota0.49327%
Prevotella intermediaPrevotellaBacteroidota0.42941%
Capnocytophaga gingivalisCapnocytophagaBacteroidota0.39582%
Streptococcus pyogenesStreptococcusBacillota0.38793%
Campylobacter curvusCampylobacterPseudomonadota0.37108%
Fusobacterium simiaeFusobacteriumFusobacteriota0.35118%
Selenomonas infelixSelenomonasBacillota0.34142%
Prevotella denticolaPrevotellaBacteroidota0.33332%
Selenomonas artemidisSelenomonasBacillota0.30370%

Reference-based 16S phylogeny

50 of 51 reported species have an eligible reference. MAFFT alignment and FastTree GTR+CAT tree, pruned to this sample. This is not a phylogeny inferred from sample reads or ASVs. Root placement is arbitrary; branch lengths are substitutions per site.

Streptococcus cristatus1.07%Streptococcus infantis0.65%Streptococcus mitis8.77%Streptococcus pneumoniae1.22%Streptococcus sanguinis0.61%Streptococcus pyogenes0.39%Gemella palaticanis0.77%Veillonella infantium0.57%Veillonella parvula7.89%Dialister invisus1.94%Selenomonas ruminantium0.95%Selenomonas sputigena1.63%Selenomonas artemidis0.30%Selenomonas noxia0.74%Selenomonas flueggei8.35%Selenomonas dianae1.66%Selenomonas infelix0.34%Eubacterium ramulus1.06%Lachnoanaerobaculum umeaense0.61%Lachnoanaerobaculum orale1.00%Lachnoanaerobaculum saburreum1.27%Filifactor alocis0.62%Filifactor villosus0.52%Fusobacterium perfoetens3.65%Fusobacterium russii1.35%Fusobacterium nucleatum6.38%Fusobacterium simiae0.35%Leptotrichia hongkongensis2.08%Campylobacter sputorum2.89%Campylobacter gracilis4.07%Campylobacter showae3.89%Campylobacter curvus0.37%Campylobacter concisus0.91%Campylobacter hyointestinalis3.75%Neisseria dentiae0.58%Neisseria macacae2.90%Neisseria meningitidis0.54%Neisseria gonorrhoeae0.69%Neisseria flavescens5.12%Neisseria elongata0.49%Aggregatibacter segnis1.09%Aggregatibacter aphrophilus0.96%Prevotella denticola0.33%Prevotella oris0.89%Prevotella intermedia0.43%Prevotella ruminicola1.98%Capnocytophaga gingivalis0.40%Capnocytophaga granulosa0.61%Streptococcus downei4.79%Streptococcus salivarius2.03%Branch length scale: 1.0631 substitutions/site across the 500-unit horizontal axis. Root arbitrary.

Public raw read files

Files contain the original full run. Downloads are hosted by ENA.

FileType / bytesMD5
SRR12522762_1.fastq.gzFASTQ / 5427532395109321f3cd776f6430e22dec4e93041

Analysis provenance

marker16S
pipelineArchaions v18.8
read_samplingFirst available reads, not random subsampling
input_sha256d940c6639d1b92a145d582b9a5eab82c618a0db7fa8fbb3061128ca56e681b51
median_read_length1415.0
reads_in_length_window9309
length_window_bp[1200, 2000]
quality_scoresUnavailable in fetched FASTA; no measured quality filtering
classifier_stats{"marker": "16s", "pipeline": "v18.8", "gpu": "RTX-PRO-6000", "input_path": "volume-fastq-gz", "reads_in": 10000, "reads_skipped": 3240, "elapsed_seconds": 0.9452969839999987, "reads_submitted": 10000, "reads_after_filter": 10000, "adapter_scan": {"sampled": 200, "matched": 2, "fraction": 0.01, "adapters_found": {"BC_FLANK": 2}, "should_trim": false}, "porechop_ran": false, "porechop_reason": "fast-path: no adapter trimming required"}

Full public metadata

All retrieved values are included. Blank source values are displayed as “Not reported”. Expand the sections below to inspect the full record.

ENA run fields (195)
run_accessionSRR12522762
ageNot reported
alignedNot reported
altitudeNot reported
assembly_qualityNot reported
assembly_softwareNot reported
bam_asperaNot reported
bam_bytesNot reported
bam_file_roleNot reported
bam_ftpNot reported
bam_galaxyNot reported
bam_md5Not reported
base_count514843865
binning_softwareNot reported
bio_materialNot reported
bisulfite_protocolNot reported
broad_scale_environmental_contextNot reported
broker_nameNot reported
cage_protocolNot reported
cell_lineNot reported
cell_typeNot reported
center_nameSUB7951364
checklistNot reported
chip_ab_providerNot reported
chip_protocolNot reported
chip_targetNot reported
collected_byNot reported
collection_datenot applicable
collection_date_endNot reported
collection_date_startNot reported
completeness_scoreNot reported
contamination_scoreNot reported
control_experimentNot reported
countryUnited Arab Emirates
cultivarNot reported
culture_collectionNot reported
datahubdcc_metagenome
depthNot reported
descriptionMinION sequencing: metagenomic
dev_stageNot reported
diseaseNot reported
dnase_protocolNot reported
ecotypeNot reported
elevationNot reported
environment_biomeNot reported
environment_featureNot reported
environment_materialNot reported
environmental_mediumNot reported
environmental_sampleNot reported
experiment_accessionSRX9012852
experiment_aliasM29-P
experiment_targetNot reported
experiment_titleMinION sequencing: metagenomic
experimental_factorNot reported
experimental_protocolNot reported
extraction_protocolNot reported
faang_library_selectionNot reported
fastq_asperafasp.sra.ebi.ac.uk:/vol1/fastq/SRR125/062/SRR12522762/SRR12522762_1.fastq.gz
fastq_bytes542753239
fastq_file_roleGENERATED_FILE
fastq_ftpftp.sra.ebi.ac.uk/vol1/fastq/SRR125/062/SRR12522762/SRR12522762_1.fastq.gz
fastq_galaxyftp.sra.ebi.ac.uk/vol1/fastq/SRR125/062/SRR12522762/SRR12522762_1.fastq.gz
fastq_md55109321f3cd776f6430e22dec4e93041
file_locationNot reported
first_created2021-10-01
first_public2021-10-01
germlineNot reported
hi_c_protocolNot reported
hostHomo sapiens
host_body_siteNot reported
host_genotypeNot reported
host_gravidityNot reported
host_growth_conditionsNot reported
host_phenotypeNot reported
host_scientific_nameHomo sapiens
host_sexNot reported
host_statusNot reported
host_tax_id9606
identified_byNot reported
instrument_modelMinION
instrument_platformOXFORD_NANOPORE
investigation_typeNot reported
isolatemixed1
isolation_sourceoral
last_updated2021-10-01
latNot reported
library_construction_protocolNot reported
library_gen_protocolNot reported
library_layoutSINGLE
library_max_fragment_sizeNot reported
library_min_fragment_sizeNot reported
library_nameM29-P
library_pcr_isolation_protocolNot reported
library_prep_dateNot reported
library_prep_date_formatNot reported
library_prep_latitudeNot reported
library_prep_locationNot reported
library_prep_longitudeNot reported
library_selectionPCR
library_sourceGENOMIC
library_strategyAMPLICON
local_environmental_contextNot reported
locationNot reported
location_endNot reported
location_startNot reported
lonNot reported
marine_regionNot reported
mating_typeNot reported
ncbi_reporting_standardMetagenome or environmental
nominal_lengthNot reported
nominal_sdevNot reported
pcr_isolation_protocolNot reported
phNot reported
project_nameNot reported
protocol_labelNot reported
read_count366571
read_strandNot reported
restriction_enzymeNot reported
restriction_enzyme_target_sequenceNot reported
restriction_siteNot reported
rna_integrity_numNot reported
rna_prep_3_protocolNot reported
rna_prep_5_protocolNot reported
rna_purity_230_ratioNot reported
rna_purity_280_ratioNot reported
rt_prep_protocolNot reported
run_aliasM29.fastq
run_dateNot reported
salinityNot reported
sample_accessionSAMN15887577
sample_aliasM29
sample_capture_statusNot reported
sample_collectionNot reported
sample_descriptionMetagenome or environmental sample from human oral metagenome
sample_materialNot reported
sample_prep_intervalNot reported
sample_prep_interval_unitsNot reported
sample_storageNot reported
sample_storage_processingNot reported
sample_titleMetagenome or environmental sample from human oral metagenome
sampling_campaignNot reported
sampling_platformNot reported
sampling_siteNot reported
scientific_namehuman oral metagenome
secondary_projectNot reported
secondary_sample_accessionSRS7263862
secondary_study_accessionSRP278853
sequencing_dateNot reported
sequencing_date_formatNot reported
sequencing_locationNot reported
sequencing_longitudeNot reported
sequencing_methodNot reported
sequencing_primer_catalogNot reported
sequencing_primer_lotNot reported
sequencing_primer_providerNot reported
serotypeNot reported
serovarNot reported
sexNot reported
specimen_voucherNot reported
sra_asperaNot reported
sra_bytesNot reported
sra_file_roleNot reported
sra_ftpNot reported
sra_galaxyNot reported
sra_md5Not reported
statuspublic
strainmixed
study_accessionPRJNA658726
study_aliasPRJNA658726
study_titleThe impact of smoking different tobacco types on the subgingival microbiome and periodontal health: a pilot study
sub_speciesNot reported
sub_strainNot reported
submission_accessionSRA1117712
submission_toolNot reported
submitted_asperaNot reported
submitted_bytesNot reported
submitted_file_roleNot reported
submitted_formatNot reported
submitted_ftpNot reported
submitted_galaxyNot reported
submitted_host_sexNot reported
submitted_md5Not reported
submitted_read_typeNot reported
surveillance_targetNot reported
tagNot reported
target_geneNot reported
tax_id447426
tax_lineage1;2787823;12908;408169;410656;447426
taxonomic_classificationNot reported
taxonomic_identity_markerNot reported
temperatureNot reported
tissue_libNot reported
tissue_typeNot reported
transposase_protocolNot reported
varietyNot reported
BioSample record and attributes
aliasM29
titleMetagenome or environmental sample from human oral metagenome
descriptionNot reported
strainmixed
collection_datenot applicable
INSDC secondary accessionSRS7263862
aliquotm4
organismhuman oral metagenome
sample_typeSubgingival
isolatemixed1
NCBI submission packageMetagenome.environmental.1.0
periodontitisno-mild
smokingS-Medwakh
hostHomo sapiens
geo_loc_nameUnited Arab Emirates
lat_lonmissing
isolation_sourceoral
BioSampleModelMetagenome or environmental
ENA-FIRST-PUBLIC2021-09-30
ENA-LAST-UPDATE2021-09-30
Curated cohort annotations and QC
runSRR12522762
sampleSAMN15887577
studyPRJNA658726
titleMetagenome or environmental sample from human oral metagenome
aliasM29
siteSubgingival
subjectNot reported
phenotypeNot reported
sexNot reported
smokingS-Medwakh
periodontitisno-mild
time_pointNot reported
negative_controlFalse
lab_modelFalse
statecomplete
reads_input10000
species_reported51
human_primaryTrue

Model-based taxonomy; research use. This report does not provide a diagnosis or a validated health score.