archAIons
archAIons · MetaBiome Project · Oral · Public research data

SRR12522758

Metagenome or environmental sample from human oral metagenome

BioSample: SAMN15887581 · Study: PRJNA658726 · Body site: Subgingival

Interactive report · NCBI SRA · ENA

78Reported species
18Reported genera

Taxonomic abundance

Relative abundance is normalized among reported taxa. No reported taxa does not establish absence of organisms.

SpeciesGenusPhylumAbundance
Streptococcus mitisStreptococcusBacillota14.76698%
Fusobacterium nucleatumFusobacteriumFusobacteriota12.16081%
Streptococcus downeiStreptococcusBacillota9.84910%
Fusobacterium perfoetensFusobacteriumFusobacteriota7.11099%
Streptococcus salivariusStreptococcusBacillota5.99769%
Parvimonas micraParvimonasBacillota4.07901%
Eubacterium ramulusEubacteriumBacillota3.49064%
Fusobacterium russiiFusobacteriumFusobacteriota2.69313%
Selenomonas flueggeiSelenomonasBacillota2.29341%
Streptococcus anginosusStreptococcusBacillota2.07420%
Gemella palaticanisGemellaBacillota2.05580%
Veillonella parvulaVeillonellaBacillota2.01925%
Streptococcus pneumoniaeStreptococcusBacillota1.62891%
Abiotrophia defectivaAbiotrophiaBacillota1.62633%
Lachnoanaerobaculum saburreumLachnoanaerobaculumBacillota1.34000%
Mogibacterium timidumMogibacteriumBacillota1.13364%
Selenomonas sputigenaSelenomonasBacillota1.05856%
Fusobacterium simiaeFusobacteriumFusobacteriota0.97964%
Streptococcus gordoniiStreptococcusBacillota0.97219%
Peptostreptococcus anaerobiusPeptostreptococcusBacillota0.95916%
Campylobacter gracilisCampylobacterPseudomonadota0.94445%
Streptococcus cristatusStreptococcusBacillota0.93181%
Mogibacterium pumilumMogibacteriumBacillota0.86593%
Lachnoanaerobaculum oraleLachnoanaerobaculumBacillota0.85819%
Prevotella ruminicolaPrevotellaBacteroidota0.82224%
Streptococcus pyogenesStreptococcusBacillota0.79579%
Gemella haemolysansGemellaBacillota0.77180%
Streptococcus infantisStreptococcusBacillota0.74880%
Dialister invisusDialisterBacillota0.62732%
Campylobacter showaeCampylobacterPseudomonadota0.59787%
Streptococcus sanguinisStreptococcusBacillota0.58691%
Campylobacter hyointestinalisCampylobacterPseudomonadota0.57995%
Campylobacter sputorumCampylobacterPseudomonadota0.52816%
Neisseria flavescensNeisseriaPseudomonadota0.52553%
Streptococcus intermediusStreptococcusBacillota0.52279%
Selenomonas dianaeSelenomonasBacillota0.51659%
Eubacterium limosumEubacteriumBacillota0.51558%
Prevotella orisPrevotellaBacteroidota0.48088%
Lachnoanaerobaculum umeaenseLachnoanaerobaculumBacillota0.45795%
Gemella morbillorumGemellaBacillota0.45610%
Fusobacterium periodonticumFusobacteriumFusobacteriota0.44674%
Mogibacterium vescumMogibacteriumBacillota0.43348%
Streptococcus oralisStreptococcusBacillota0.39376%
Capnocytophaga canisCapnocytophagaBacteroidota0.36761%
Streptococcus gallolyticusStreptococcusBacillota0.35825%
Haemophilus parainfluenzaeHaemophilusPseudomonadota0.34245%
Mogibacterium diversumMogibacteriumBacillota0.32628%
Selenomonas ruminantiumSelenomonasBacillota0.31068%
Neisseria macacaeNeisseriaPseudomonadota0.30379%
Streptococcus parasanguinisStreptococcusBacillota0.27008%
Streptococcus suisStreptococcusBacillota0.26379%
Streptococcus constellatusStreptococcusBacillota0.24726%
Eubacterium xylanophilumEubacteriumBacillota0.24611%
Haemophilus haemolyticusHaemophilusPseudomonadota0.24170%
Streptococcus alactolyticusStreptococcusBacillota0.24120%
Veillonella infantiumVeillonellaBacillota0.22174%
Filifactor alocisFilifactorBacillota0.21410%
Prevotella denticolaPrevotellaBacteroidota0.20295%
Streptococcus orisrattiStreptococcusBacillota0.20221%
Prevotella buccalisPrevotellaBacteroidota0.20105%
Mogibacterium neglectumMogibacteriumBacillota0.20026%
Streptococcus marmotaeStreptococcusBacillota0.19822%
Streptococcus australisStreptococcusBacillota0.19554%
Eubacterium pyruvativoransEubacteriumBacillota0.19403%
Dialister hominisDialisterBacillota0.16868%
Neisseria mucosaNeisseriaPseudomonadota0.16272%
Eubacterium multiformeEubacteriumBacillota0.16069%
Streptococcus macacaeStreptococcusBacillota0.16038%
Eubacterium barkeriEubacteriumBacillota0.15589%
Streptococcus sobrinusStreptococcusBacillota0.15273%
Peptostreptococcus stomatisPeptostreptococcusBacillota0.14873%
Streptococcus milleriStreptococcusBacillota0.14038%
Streptococcus perorisStreptococcusBacillota0.12785%
Eubacterium callanderiEubacteriumBacillota0.12276%
Filifactor villosusFilifactorBacillota0.12223%
Fusobacterium hwasookiiFusobacteriumFusobacteriota0.11166%
Gemella sanguinisGemellaBacillota0.10988%
Gemella cuniculiGemellaBacillota0.10807%

Reference-based 16S phylogeny

77 of 78 reported species have an eligible reference. MAFFT alignment and FastTree GTR+CAT tree, pruned to this sample. This is not a phylogeny inferred from sample reads or ASVs. Root placement is arbitrary; branch lengths are substitutions per site.

Streptococcus anginosus2.07%Streptococcus milleri0.14%Streptococcus constellatus0.25%Streptococcus intermedius0.52%Streptococcus cristatus0.93%Streptococcus parasanguinis0.27%Streptococcus peroris0.13%Streptococcus infantis0.75%Streptococcus oralis0.39%Streptococcus mitis14.77%Streptococcus pneumoniae1.63%Streptococcus australis0.20%Streptococcus marmotae0.20%Streptococcus gordonii0.97%Streptococcus sanguinis0.59%Streptococcus suis0.26%Streptococcus alactolyticus0.24%Streptococcus gallolyticus0.36%Streptococcus orisratti0.20%Streptococcus pyogenes0.80%Abiotrophia defectiva1.63%Gemella palaticanis2.06%Gemella cuniculi0.11%Gemella haemolysans0.77%Gemella morbillorum0.46%Gemella sanguinis0.11%Veillonella infantium0.22%Veillonella parvula2.02%Dialister invisus0.63%Dialister hominis0.17%Selenomonas ruminantium0.31%Selenomonas sputigena1.06%Selenomonas flueggei2.29%Selenomonas dianae0.52%Eubacterium xylanophilum0.25%Eubacterium ramulus3.49%Lachnoanaerobaculum umeaense0.46%Lachnoanaerobaculum orale0.86%Lachnoanaerobaculum saburreum1.34%Eubacterium multiforme0.16%Eubacterium barkeri0.16%Eubacterium callanderi0.12%Eubacterium limosum0.52%Parvimonas micra4.08%Peptostreptococcus anaerobius0.96%Peptostreptococcus stomatis0.15%Filifactor alocis0.21%Filifactor villosus0.12%Mogibacterium pumilum0.87%Mogibacterium timidum1.13%Mogibacterium neglectum0.20%Mogibacterium diversum0.33%Mogibacterium vescum0.43%Eubacterium pyruvativorans0.19%Fusobacterium perfoetens7.11%Fusobacterium russii2.69%Fusobacterium periodonticum0.45%Fusobacterium hwasookii0.11%Fusobacterium nucleatum12.16%Fusobacterium simiae0.98%Campylobacter sputorum0.53%Campylobacter gracilis0.94%Campylobacter showae0.60%Campylobacter hyointestinalis0.58%Neisseria mucosa0.16%Neisseria macacae0.30%Neisseria flavescens0.53%Haemophilus parainfluenzae0.34%Haemophilus haemolyticus0.24%Prevotella denticola0.20%Prevotella oris0.48%Prevotella buccalis0.20%Prevotella ruminicola0.82%Streptococcus macacae0.16%Streptococcus sobrinus0.15%Streptococcus downei9.85%Streptococcus salivarius6.00%Branch length scale: 1.0406 substitutions/site across the 500-unit horizontal axis. Root arbitrary.

Public raw read files

Files contain the original full run. Downloads are hosted by ENA.

FileType / bytesMD5
SRR12522758_1.fastq.gzFASTQ / 4560979676408cf783cb9e26286c8740c0c7397d1

Analysis provenance

marker16S
pipelineArchaions v18.8
read_samplingFirst available reads, not random subsampling
input_sha256ebbf08a486736f241fe354f341d9f21117f1caee48bd8e432e61fc3f2a423cbb
median_read_length1417.0
reads_in_length_window27901
length_window_bp[1200, 2000]
quality_scoresUnavailable in fetched FASTA; no measured quality filtering
classifier_stats{"marker": "16s", "pipeline": "v18.8", "gpu": "RTX-PRO-6000", "input_path": "volume-fastq-gz", "reads_in": 30000, "reads_skipped": 9984, "elapsed_seconds": 3.2933095069999965, "reads_submitted": 30000, "reads_after_filter": 30000, "adapter_scan": {"sampled": 200, "matched": 1, "fraction": 0.005, "adapters_found": {"BC_FLANK": 1}, "should_trim": false}, "porechop_ran": false, "porechop_reason": "fast-path: no adapter trimming required"}

Full public metadata

All retrieved values are included. Blank source values are displayed as “Not reported”. Expand the sections below to inspect the full record.

ENA run fields (195)
run_accessionSRR12522758
ageNot reported
alignedNot reported
altitudeNot reported
assembly_qualityNot reported
assembly_softwareNot reported
bam_asperaNot reported
bam_bytesNot reported
bam_file_roleNot reported
bam_ftpNot reported
bam_galaxyNot reported
bam_md5Not reported
base_count436304539
binning_softwareNot reported
bio_materialNot reported
bisulfite_protocolNot reported
broad_scale_environmental_contextNot reported
broker_nameNot reported
cage_protocolNot reported
cell_lineNot reported
cell_typeNot reported
center_nameSUB7951364
checklistNot reported
chip_ab_providerNot reported
chip_protocolNot reported
chip_targetNot reported
collected_byNot reported
collection_datenot applicable
collection_date_endNot reported
collection_date_startNot reported
completeness_scoreNot reported
contamination_scoreNot reported
control_experimentNot reported
countryUnited Arab Emirates
cultivarNot reported
culture_collectionNot reported
datahubdcc_metagenome
depthNot reported
descriptionMinION sequencing: metagenomic
dev_stageNot reported
diseaseNot reported
dnase_protocolNot reported
ecotypeNot reported
elevationNot reported
environment_biomeNot reported
environment_featureNot reported
environment_materialNot reported
environmental_mediumNot reported
environmental_sampleNot reported
experiment_accessionSRX9012856
experiment_aliasM64-P
experiment_targetNot reported
experiment_titleMinION sequencing: metagenomic
experimental_factorNot reported
experimental_protocolNot reported
extraction_protocolNot reported
faang_library_selectionNot reported
fastq_asperafasp.sra.ebi.ac.uk:/vol1/fastq/SRR125/058/SRR12522758/SRR12522758_1.fastq.gz
fastq_bytes456097967
fastq_file_roleGENERATED_FILE
fastq_ftpftp.sra.ebi.ac.uk/vol1/fastq/SRR125/058/SRR12522758/SRR12522758_1.fastq.gz
fastq_galaxyftp.sra.ebi.ac.uk/vol1/fastq/SRR125/058/SRR12522758/SRR12522758_1.fastq.gz
fastq_md56408cf783cb9e26286c8740c0c7397d1
file_locationNot reported
first_created2021-10-01
first_public2021-10-01
germlineNot reported
hi_c_protocolNot reported
hostHomo sapiens
host_body_siteNot reported
host_genotypeNot reported
host_gravidityNot reported
host_growth_conditionsNot reported
host_phenotypeNot reported
host_scientific_nameHomo sapiens
host_sexNot reported
host_statusNot reported
host_tax_id9606
identified_byNot reported
instrument_modelMinION
instrument_platformOXFORD_NANOPORE
investigation_typeNot reported
isolatemixed1
isolation_sourceoral
last_updated2021-10-01
latNot reported
library_construction_protocolNot reported
library_gen_protocolNot reported
library_layoutSINGLE
library_max_fragment_sizeNot reported
library_min_fragment_sizeNot reported
library_nameM64-P
library_pcr_isolation_protocolNot reported
library_prep_dateNot reported
library_prep_date_formatNot reported
library_prep_latitudeNot reported
library_prep_locationNot reported
library_prep_longitudeNot reported
library_selectionPCR
library_sourceGENOMIC
library_strategyAMPLICON
local_environmental_contextNot reported
locationNot reported
location_endNot reported
location_startNot reported
lonNot reported
marine_regionNot reported
mating_typeNot reported
ncbi_reporting_standardMetagenome or environmental
nominal_lengthNot reported
nominal_sdevNot reported
pcr_isolation_protocolNot reported
phNot reported
project_nameNot reported
protocol_labelNot reported
read_count312467
read_strandNot reported
restriction_enzymeNot reported
restriction_enzyme_target_sequenceNot reported
restriction_siteNot reported
rna_integrity_numNot reported
rna_prep_3_protocolNot reported
rna_prep_5_protocolNot reported
rna_purity_230_ratioNot reported
rna_purity_280_ratioNot reported
rt_prep_protocolNot reported
run_aliasM64.fastq
run_dateNot reported
salinityNot reported
sample_accessionSAMN15887581
sample_aliasM64
sample_capture_statusNot reported
sample_collectionNot reported
sample_descriptionMetagenome or environmental sample from human oral metagenome
sample_materialNot reported
sample_prep_intervalNot reported
sample_prep_interval_unitsNot reported
sample_storageNot reported
sample_storage_processingNot reported
sample_titleMetagenome or environmental sample from human oral metagenome
sampling_campaignNot reported
sampling_platformNot reported
sampling_siteNot reported
scientific_namehuman oral metagenome
secondary_projectNot reported
secondary_sample_accessionSRS7263868
secondary_study_accessionSRP278853
sequencing_dateNot reported
sequencing_date_formatNot reported
sequencing_locationNot reported
sequencing_longitudeNot reported
sequencing_methodNot reported
sequencing_primer_catalogNot reported
sequencing_primer_lotNot reported
sequencing_primer_providerNot reported
serotypeNot reported
serovarNot reported
sexNot reported
specimen_voucherNot reported
sra_asperaNot reported
sra_bytesNot reported
sra_file_roleNot reported
sra_ftpNot reported
sra_galaxyNot reported
sra_md5Not reported
statuspublic
strainmixed
study_accessionPRJNA658726
study_aliasPRJNA658726
study_titleThe impact of smoking different tobacco types on the subgingival microbiome and periodontal health: a pilot study
sub_speciesNot reported
sub_strainNot reported
submission_accessionSRA1117712
submission_toolNot reported
submitted_asperaNot reported
submitted_bytesNot reported
submitted_file_roleNot reported
submitted_formatNot reported
submitted_ftpNot reported
submitted_galaxyNot reported
submitted_host_sexNot reported
submitted_md5Not reported
submitted_read_typeNot reported
surveillance_targetNot reported
tagNot reported
target_geneNot reported
tax_id447426
tax_lineage1;2787823;12908;408169;410656;447426
taxonomic_classificationNot reported
taxonomic_identity_markerNot reported
temperatureNot reported
tissue_libNot reported
tissue_typeNot reported
transposase_protocolNot reported
varietyNot reported
BioSample record and attributes
aliasM64
titleMetagenome or environmental sample from human oral metagenome
descriptionNot reported
strainmixed
collection_datenot applicable
INSDC secondary accessionSRS7263868
aliquotm8
organismhuman oral metagenome
sample_typeSubgingival
isolatemixed1
NCBI submission packageMetagenome.environmental.1.0
periodontitisno-mild
smokingS-Medwakh
hostHomo sapiens
geo_loc_nameUnited Arab Emirates
lat_lonmissing
isolation_sourceoral
BioSampleModelMetagenome or environmental
ENA-FIRST-PUBLIC2021-09-30
ENA-LAST-UPDATE2021-09-30
Curated cohort annotations and QC
runSRR12522758
sampleSAMN15887581
studyPRJNA658726
titleMetagenome or environmental sample from human oral metagenome
aliasM64
siteSubgingival
subjectNot reported
phenotypeNot reported
sexNot reported
smokingS-Medwakh
periodontitisno-mild
time_pointNot reported
negative_controlFalse
lab_modelFalse
statecomplete
reads_input30000
species_reported78
human_primaryTrue

Model-based taxonomy; research use. This report does not provide a diagnosis or a validated health score.