archAIons
archAIons · MetaBiome Project · Oral · Public research data

SRR12522754

Metagenome or environmental sample from human oral metagenome

BioSample: SAMN15887584 · Study: PRJNA658726 · Body site: Subgingival

Interactive report · NCBI SRA · ENA

54Reported species
21Reported genera

Taxonomic abundance

Relative abundance is normalized among reported taxa. No reported taxa does not establish absence of organisms.

SpeciesGenusPhylumAbundance
Streptococcus mitisStreptococcusBacillota20.27310%
Fusobacterium nucleatumFusobacteriumFusobacteriota8.04657%
Streptococcus downeiStreptococcusBacillota6.67333%
Fusobacterium perfoetensFusobacteriumFusobacteriota6.05267%
Capnocytophaga canisCapnocytophagaBacteroidota4.53759%
Veillonella parvulaVeillonellaBacillota4.17243%
Gemella palaticanisGemellaBacillota3.45019%
Streptococcus pneumoniaeStreptococcusBacillota3.37518%
Streptococcus salivariusStreptococcusBacillota3.35027%
Leptotrichia hongkongensisLeptotrichiaFusobacteriota2.28668%
Selenomonas flueggeiSelenomonasBacillota2.15060%
Campylobacter gracilisCampylobacterPseudomonadota1.93189%
Fusobacterium russiiFusobacteriumFusobacteriota1.90421%
Campylobacter hyointestinalisCampylobacterPseudomonadota1.74385%
Eubacterium ramulusEubacteriumBacillota1.74301%
Capnocytophaga gingivalisCapnocytophagaBacteroidota1.38760%
Campylobacter sputorumCampylobacterPseudomonadota1.37755%
Haemophilus parainfluenzaeHaemophilusPseudomonadota1.33592%
Campylobacter showaeCampylobacterPseudomonadota1.32524%
Gemella haemolysansGemellaBacillota1.27449%
Neisseria flavescensNeisseriaPseudomonadota1.25699%
Lachnoanaerobaculum saburreumLachnoanaerobaculumBacillota1.20981%
Capnocytophaga granulosaCapnocytophagaBacteroidota1.17405%
Tannerella forsythiaTannerellaBacteroidota1.15459%
Streptococcus oralisStreptococcusBacillota1.09725%
Streptococcus infantisStreptococcusBacillota1.00336%
Parvimonas micraParvimonasBacillota0.96692%
Filifactor alocisFilifactorBacillota0.83529%
Selenomonas sputigenaSelenomonasBacillota0.82687%
Peptostreptococcus anaerobiusPeptostreptococcusBacillota0.80153%
Prevotella ruminicolaPrevotellaBacteroidota0.72081%
Haemophilus haemolyticusHaemophilusPseudomonadota0.69970%
Fusobacterium simiaeFusobacteriumFusobacteriota0.63964%
Porphyromonas catoniaePorphyromonasBacteroidota0.63616%
Lachnoanaerobaculum oraleLachnoanaerobaculumBacillota0.63612%
Veillonella infantiumVeillonellaBacillota0.61004%
Streptococcus cristatusStreptococcusBacillota0.60910%
Gemella morbillorumGemellaBacillota0.58212%
Dialister invisusDialisterBacillota0.54832%
Selenomonas dianaeSelenomonasBacillota0.50853%
Streptococcus intermediusStreptococcusBacillota0.48515%
Streptococcus pyogenesStreptococcusBacillota0.47423%
Lachnoanaerobaculum umeaenseLachnoanaerobaculumBacillota0.46959%
Leptotrichia shahiiLeptotrichiaFusobacteriota0.44869%
Filifactor villosusFilifactorBacillota0.43697%
Porphyromonas macacaePorphyromonasBacteroidota0.39754%
Prevotella loescheiiPrevotellaBacteroidota0.38606%
Fusobacterium periodonticumFusobacteriumFusobacteriota0.34160%
Neisseria macacaeNeisseriaPseudomonadota0.33334%
Granulicatella adiacensGranulicatellaBacillota0.31807%
Gemella cuniculiGemellaBacillota0.26572%
Campylobacter curvusCampylobacterPseudomonadota0.25940%
Campylobacter concisusCampylobacterPseudomonadota0.25777%
Rothia aeriaRothiaActinomycetota0.21628%

Reference-based 16S phylogeny

53 of 54 reported species have an eligible reference. MAFFT alignment and FastTree GTR+CAT tree, pruned to this sample. This is not a phylogeny inferred from sample reads or ASVs. Root placement is arbitrary; branch lengths are substitutions per site.

Streptococcus intermedius0.49%Streptococcus cristatus0.61%Streptococcus infantis1.00%Streptococcus oralis1.10%Streptococcus mitis20.27%Streptococcus pneumoniae3.38%Streptococcus pyogenes0.47%Granulicatella adiacens0.32%Gemella palaticanis3.45%Gemella cuniculi0.27%Gemella haemolysans1.27%Gemella morbillorum0.58%Veillonella infantium0.61%Veillonella parvula4.17%Dialister invisus0.55%Selenomonas sputigena0.83%Selenomonas flueggei2.15%Selenomonas dianae0.51%Eubacterium ramulus1.74%Lachnoanaerobaculum umeaense0.47%Lachnoanaerobaculum orale0.64%Lachnoanaerobaculum saburreum1.21%Parvimonas micra0.97%Peptostreptococcus anaerobius0.80%Filifactor alocis0.84%Filifactor villosus0.44%Fusobacterium perfoetens6.05%Fusobacterium russii1.90%Fusobacterium periodonticum0.34%Fusobacterium nucleatum8.05%Fusobacterium simiae0.64%Leptotrichia hongkongensis2.29%Leptotrichia shahii0.45%Campylobacter sputorum1.38%Campylobacter gracilis1.93%Campylobacter showae1.33%Campylobacter curvus0.26%Campylobacter concisus0.26%Campylobacter hyointestinalis1.74%Neisseria macacae0.33%Neisseria flavescens1.26%Haemophilus parainfluenzae1.34%Haemophilus haemolyticus0.70%Porphyromonas catoniae0.64%Porphyromonas macacae0.40%Tannerella forsythia1.15%Prevotella loescheii0.39%Prevotella ruminicola0.72%Capnocytophaga gingivalis1.39%Capnocytophaga granulosa1.17%Rothia aeria0.22%Streptococcus downei6.67%Streptococcus salivarius3.35%Branch length scale: 1.0488 substitutions/site across the 500-unit horizontal axis. Root arbitrary.

Public raw read files

Files contain the original full run. Downloads are hosted by ENA.

FileType / bytesMD5
SRR12522754_1.fastq.gzFASTQ / 6519529446c95c71a416e82e0ec7baca858d9697b

Analysis provenance

marker16S
pipelineArchaions v18.8
read_samplingFirst available reads, not random subsampling
input_sha256602164dceee426ae3249f72ac4afea804f6cc76597eaaac2e316c0d38d109a96
median_read_length1420.0
reads_in_length_window9547
length_window_bp[1200, 2000]
quality_scoresUnavailable in fetched FASTA; no measured quality filtering
classifier_stats{"marker": "16s", "pipeline": "v18.8", "gpu": "RTX-PRO-6000", "input_path": "volume-fastq-gz", "reads_in": 10000, "reads_skipped": 1841, "elapsed_seconds": 1.1041596570000252, "reads_submitted": 10000, "reads_after_filter": 10000, "adapter_scan": {"sampled": 200, "matched": 4, "fraction": 0.02, "adapters_found": {"BC_FLANK": 4}, "should_trim": false}, "porechop_ran": false, "porechop_reason": "fast-path: no adapter trimming required"}

Full public metadata

All retrieved values are included. Blank source values are displayed as “Not reported”. Expand the sections below to inspect the full record.

ENA run fields (195)
run_accessionSRR12522754
ageNot reported
alignedNot reported
altitudeNot reported
assembly_qualityNot reported
assembly_softwareNot reported
bam_asperaNot reported
bam_bytesNot reported
bam_file_roleNot reported
bam_ftpNot reported
bam_galaxyNot reported
bam_md5Not reported
base_count624779136
binning_softwareNot reported
bio_materialNot reported
bisulfite_protocolNot reported
broad_scale_environmental_contextNot reported
broker_nameNot reported
cage_protocolNot reported
cell_lineNot reported
cell_typeNot reported
center_nameSUB7951364
checklistNot reported
chip_ab_providerNot reported
chip_protocolNot reported
chip_targetNot reported
collected_byNot reported
collection_datenot applicable
collection_date_endNot reported
collection_date_startNot reported
completeness_scoreNot reported
contamination_scoreNot reported
control_experimentNot reported
countryUnited Arab Emirates
cultivarNot reported
culture_collectionNot reported
datahubdcc_metagenome
depthNot reported
descriptionMinION sequencing: metagenomic
dev_stageNot reported
diseaseNot reported
dnase_protocolNot reported
ecotypeNot reported
elevationNot reported
environment_biomeNot reported
environment_featureNot reported
environment_materialNot reported
environmental_mediumNot reported
environmental_sampleNot reported
experiment_accessionSRX9012860
experiment_aliasSH14-P
experiment_targetNot reported
experiment_titleMinION sequencing: metagenomic
experimental_factorNot reported
experimental_protocolNot reported
extraction_protocolNot reported
faang_library_selectionNot reported
fastq_asperafasp.sra.ebi.ac.uk:/vol1/fastq/SRR125/054/SRR12522754/SRR12522754_1.fastq.gz
fastq_bytes651952944
fastq_file_roleGENERATED_FILE
fastq_ftpftp.sra.ebi.ac.uk/vol1/fastq/SRR125/054/SRR12522754/SRR12522754_1.fastq.gz
fastq_galaxyftp.sra.ebi.ac.uk/vol1/fastq/SRR125/054/SRR12522754/SRR12522754_1.fastq.gz
fastq_md56c95c71a416e82e0ec7baca858d9697b
file_locationNot reported
first_created2021-10-01
first_public2021-10-01
germlineNot reported
hi_c_protocolNot reported
hostHomo sapiens
host_body_siteNot reported
host_genotypeNot reported
host_gravidityNot reported
host_growth_conditionsNot reported
host_phenotypeNot reported
host_scientific_nameHomo sapiens
host_sexNot reported
host_statusNot reported
host_tax_id9606
identified_byNot reported
instrument_modelMinION
instrument_platformOXFORD_NANOPORE
investigation_typeNot reported
isolatemixed1
isolation_sourceoral
last_updated2021-10-01
latNot reported
library_construction_protocolNot reported
library_gen_protocolNot reported
library_layoutSINGLE
library_max_fragment_sizeNot reported
library_min_fragment_sizeNot reported
library_nameSH14-P
library_pcr_isolation_protocolNot reported
library_prep_dateNot reported
library_prep_date_formatNot reported
library_prep_latitudeNot reported
library_prep_locationNot reported
library_prep_longitudeNot reported
library_selectionPCR
library_sourceGENOMIC
library_strategyAMPLICON
local_environmental_contextNot reported
locationNot reported
location_endNot reported
location_startNot reported
lonNot reported
marine_regionNot reported
mating_typeNot reported
ncbi_reporting_standardMetagenome or environmental
nominal_lengthNot reported
nominal_sdevNot reported
pcr_isolation_protocolNot reported
phNot reported
project_nameNot reported
protocol_labelNot reported
read_count447487
read_strandNot reported
restriction_enzymeNot reported
restriction_enzyme_target_sequenceNot reported
restriction_siteNot reported
rna_integrity_numNot reported
rna_prep_3_protocolNot reported
rna_prep_5_protocolNot reported
rna_purity_230_ratioNot reported
rna_purity_280_ratioNot reported
rt_prep_protocolNot reported
run_aliasSH14.fastq
run_dateNot reported
salinityNot reported
sample_accessionSAMN15887584
sample_aliasSH14
sample_capture_statusNot reported
sample_collectionNot reported
sample_descriptionMetagenome or environmental sample from human oral metagenome
sample_materialNot reported
sample_prep_intervalNot reported
sample_prep_interval_unitsNot reported
sample_storageNot reported
sample_storage_processingNot reported
sample_titleMetagenome or environmental sample from human oral metagenome
sampling_campaignNot reported
sampling_platformNot reported
sampling_siteNot reported
scientific_namehuman oral metagenome
secondary_projectNot reported
secondary_sample_accessionSRS7263872
secondary_study_accessionSRP278853
sequencing_dateNot reported
sequencing_date_formatNot reported
sequencing_locationNot reported
sequencing_longitudeNot reported
sequencing_methodNot reported
sequencing_primer_catalogNot reported
sequencing_primer_lotNot reported
sequencing_primer_providerNot reported
serotypeNot reported
serovarNot reported
sexNot reported
specimen_voucherNot reported
sra_asperaNot reported
sra_bytesNot reported
sra_file_roleNot reported
sra_ftpNot reported
sra_galaxyNot reported
sra_md5Not reported
statuspublic
strainmixed
study_accessionPRJNA658726
study_aliasPRJNA658726
study_titleThe impact of smoking different tobacco types on the subgingival microbiome and periodontal health: a pilot study
sub_speciesNot reported
sub_strainNot reported
submission_accessionSRA1117712
submission_toolNot reported
submitted_asperaNot reported
submitted_bytesNot reported
submitted_file_roleNot reported
submitted_formatNot reported
submitted_ftpNot reported
submitted_galaxyNot reported
submitted_host_sexNot reported
submitted_md5Not reported
submitted_read_typeNot reported
surveillance_targetNot reported
tagNot reported
target_geneNot reported
tax_id447426
tax_lineage1;2787823;12908;408169;410656;447426
taxonomic_classificationNot reported
taxonomic_identity_markerNot reported
temperatureNot reported
tissue_libNot reported
tissue_typeNot reported
transposase_protocolNot reported
varietyNot reported
BioSample record and attributes
aliasSH14
titleMetagenome or environmental sample from human oral metagenome
descriptionNot reported
strainmixed
collection_datenot applicable
INSDC secondary accessionSRS7263872
aliquots1
organismhuman oral metagenome
sample_typeSubgingival
isolatemixed1
NCBI submission packageMetagenome.environmental.1.0
periodontitisno-mild
smokingS-Shisha
hostHomo sapiens
geo_loc_nameUnited Arab Emirates
lat_lonmissing
isolation_sourceoral
BioSampleModelMetagenome or environmental
ENA-FIRST-PUBLIC2021-09-30
ENA-LAST-UPDATE2021-09-30
Curated cohort annotations and QC
runSRR12522754
sampleSAMN15887584
studyPRJNA658726
titleMetagenome or environmental sample from human oral metagenome
aliasSH14
siteSubgingival
subjectNot reported
phenotypeNot reported
sexNot reported
smokingS-Shisha
periodontitisno-mild
time_pointNot reported
negative_controlFalse
lab_modelFalse
statecomplete
reads_input10000
species_reported54
human_primaryTrue

Model-based taxonomy; research use. This report does not provide a diagnosis or a validated health score.