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archAIons · MetaBiome Project · Oral · Public research data

SRR12522753

Metagenome or environmental sample from human oral metagenome

BioSample: SAMN15887585 · Study: PRJNA658726 · Body site: Subgingival

Interactive report · NCBI SRA · ENA

83Reported species
19Reported genera

Taxonomic abundance

Relative abundance is normalized among reported taxa. No reported taxa does not establish absence of organisms.

SpeciesGenusPhylumAbundance
Veillonella parvulaVeillonellaBacillota15.27381%
Streptococcus mitisStreptococcusBacillota8.25083%
Fusobacterium nucleatumFusobacteriumFusobacteriota5.88474%
Selenomonas flueggeiSelenomonasBacillota4.99065%
Streptococcus downeiStreptococcusBacillota4.98589%
Campylobacter hyointestinalisCampylobacterPseudomonadota3.48357%
Fusobacterium perfoetensFusobacteriumFusobacteriota3.21837%
Campylobacter gracilisCampylobacterPseudomonadota3.19939%
Haemophilus haemolyticusHaemophilusPseudomonadota2.84016%
Campylobacter sputorumCampylobacterPseudomonadota2.73708%
Streptococcus salivariusStreptococcusBacillota2.58641%
Campylobacter showaeCampylobacterPseudomonadota2.53898%
Gemella palaticanisGemellaBacillota2.44436%
Parvimonas micraParvimonasBacillota2.13497%
Capnocytophaga canisCapnocytophagaBacteroidota1.94841%
Haemophilus parainfluenzaeHaemophilusPseudomonadota1.81013%
Veillonella infantiumVeillonellaBacillota1.65650%
Selenomonas sputigenaSelenomonasBacillota1.53255%
Eubacterium ramulusEubacteriumBacillota1.47181%
Fusobacterium russiiFusobacteriumFusobacteriota1.20847%
Streptococcus pneumoniaeStreptococcusBacillota1.03668%
Gemella haemolysansGemellaBacillota0.97172%
Selenomonas dianaeSelenomonasBacillota0.94762%
Streptococcus cristatusStreptococcusBacillota0.85517%
Aggregatibacter segnisAggregatibacterPseudomonadota0.82382%
Veillonella disparVeillonellaBacillota0.81781%
Dialister invisusDialisterBacillota0.79956%
Prevotella ruminicolaPrevotellaBacteroidota0.79747%
Aggregatibacter aphrophilusAggregatibacterPseudomonadota0.78973%
Campylobacter concisusCampylobacterPseudomonadota0.73418%
Lachnoanaerobaculum oraleLachnoanaerobaculumBacillota0.70965%
Leptotrichia hongkongensisLeptotrichiaFusobacteriota0.69448%
Lachnoanaerobaculum saburreumLachnoanaerobaculumBacillota0.67920%
Selenomonas noxiaSelenomonasBacillota0.65962%
Haemophilus aegyptiusHaemophilusPseudomonadota0.60844%
Neisseria flavescensNeisseriaPseudomonadota0.60784%
Streptococcus infantisStreptococcusBacillota0.53861%
Filifactor alocisFilifactorBacillota0.50666%
Gemella morbillorumGemellaBacillota0.48899%
Capnocytophaga gingivalisCapnocytophagaBacteroidota0.47741%
Haemophilus parahaemolyticusHaemophilusPseudomonadota0.44613%
Streptococcus intermediusStreptococcusBacillota0.43899%
Campylobacter rectusCampylobacterPseudomonadota0.43792%
Granulicatella adiacensGranulicatellaBacillota0.42699%
Prevotella orisPrevotellaBacteroidota0.41961%
Streptococcus pyogenesStreptococcusBacillota0.39953%
Eubacterium limosumEubacteriumBacillota0.39919%
Capnocytophaga granulosaCapnocytophagaBacteroidota0.38677%
Lachnoanaerobaculum umeaenseLachnoanaerobaculumBacillota0.36933%
Streptococcus oralisStreptococcusBacillota0.36798%
Filifactor villosusFilifactorBacillota0.36123%
Veillonella atypicaVeillonellaBacillota0.35369%
Neisseria macacaeNeisseriaPseudomonadota0.34361%
Fusobacterium simiaeFusobacteriumFusobacteriota0.33333%
Campylobacter curvusCampylobacterPseudomonadota0.32998%
Veillonella rodentiumVeillonellaBacillota0.32384%
Streptococcus gordoniiStreptococcusBacillota0.31222%
Selenomonas ruminantiumSelenomonasBacillota0.29690%
Streptococcus anginosusStreptococcusBacillota0.29294%
Fusobacterium periodonticumFusobacteriumFusobacteriota0.28725%
Streptococcus sanguinisStreptococcusBacillota0.27214%
Porphyromonas catoniaePorphyromonasBacteroidota0.26919%
Dialister hominisDialisterBacillota0.26919%
Selenomonas infelixSelenomonasBacillota0.23197%
Haemophilus influenzaeHaemophilusPseudomonadota0.22550%
Prevotella intermediaPrevotellaBacteroidota0.22095%
Streptococcus alactolyticusStreptococcusBacillota0.20408%
Streptococcus suisStreptococcusBacillota0.19484%
Prevotella loescheiiPrevotellaBacteroidota0.18113%
Haemophilus paraphrohaemolyticusHaemophilusPseudomonadota0.17293%
Leptotrichia shahiiLeptotrichiaFusobacteriota0.17055%
Campylobacter ureolyticusCampylobacterPseudomonadota0.15188%
Gemella cuniculiGemellaBacillota0.15138%
Eubacterium xylanophilumEubacteriumBacillota0.14377%
Leptotrichia hofstadiiLeptotrichiaFusobacteriota0.14212%
Lachnoanaerobaculum gingivalisLachnoanaerobaculumBacillota0.14173%
Streptococcus constellatusStreptococcusBacillota0.12598%
Prevotella nigrescensPrevotellaBacteroidota0.12129%
Streptococcus australisStreptococcusBacillota0.11642%
Streptococcus marmotaeStreptococcusBacillota0.10986%
Aggregatibacter actinomycetemcomitansAggregatibacterPseudomonadota0.10828%
Streptococcus parasanguinisStreptococcusBacillota0.10794%
Campylobacter hominisCampylobacterPseudomonadota0.09772%

Reference-based 16S phylogeny

82 of 83 reported species have an eligible reference. MAFFT alignment and FastTree GTR+CAT tree, pruned to this sample. This is not a phylogeny inferred from sample reads or ASVs. Root placement is arbitrary; branch lengths are substitutions per site.

Streptococcus anginosus0.29%Streptococcus constellatus0.13%Streptococcus intermedius0.44%Streptococcus cristatus0.86%Streptococcus parasanguinis0.11%Streptococcus infantis0.54%Streptococcus oralis0.37%Streptococcus mitis8.25%Streptococcus pneumoniae1.04%Streptococcus australis0.12%Streptococcus marmotae0.11%Streptococcus gordonii0.31%Streptococcus sanguinis0.27%Streptococcus suis0.19%Streptococcus alactolyticus0.20%Streptococcus pyogenes0.40%Granulicatella adiacens0.43%Gemella palaticanis2.44%Gemella cuniculi0.15%Gemella haemolysans0.97%Gemella morbillorum0.49%Veillonella dispar0.82%Veillonella infantium1.66%Veillonella parvula15.27%Veillonella atypica0.35%Veillonella rodentium0.32%Dialister invisus0.80%Dialister hominis0.27%Selenomonas ruminantium0.30%Selenomonas sputigena1.53%Selenomonas noxia0.66%Selenomonas flueggei4.99%Selenomonas dianae0.95%Selenomonas infelix0.23%Eubacterium xylanophilum0.14%Eubacterium ramulus1.47%Lachnoanaerobaculum gingivalis0.14%Lachnoanaerobaculum umeaense0.37%Lachnoanaerobaculum orale0.71%Lachnoanaerobaculum saburreum0.68%Eubacterium limosum0.40%Parvimonas micra2.13%Filifactor alocis0.51%Filifactor villosus0.36%Fusobacterium perfoetens3.22%Fusobacterium russii1.21%Fusobacterium periodonticum0.29%Fusobacterium nucleatum5.88%Fusobacterium simiae0.33%Leptotrichia hongkongensis0.69%Leptotrichia shahii0.17%Leptotrichia hofstadii0.14%Campylobacter hominis0.10%Campylobacter sputorum2.74%Campylobacter gracilis3.20%Campylobacter rectus0.44%Campylobacter showae2.54%Campylobacter curvus0.33%Campylobacter concisus0.73%Campylobacter hyointestinalis3.48%Campylobacter ureolyticus0.15%Neisseria macacae0.34%Neisseria flavescens0.61%Haemophilus parainfluenzae1.81%Haemophilus parahaemolyticus0.45%Haemophilus paraphrohaemolyticus0.17%Haemophilus haemolyticus2.84%Haemophilus aegyptius0.61%Haemophilus influenzae0.23%Aggregatibacter segnis0.82%Aggregatibacter actinomycetemcomitans0.11%Aggregatibacter aphrophilus0.79%Porphyromonas catoniae0.27%Prevotella oris0.42%Prevotella intermedia0.22%Prevotella nigrescens0.12%Prevotella loescheii0.18%Prevotella ruminicola0.80%Capnocytophaga gingivalis0.48%Capnocytophaga granulosa0.39%Streptococcus downei4.99%Streptococcus salivarius2.59%Branch length scale: 1.0631 substitutions/site across the 500-unit horizontal axis. Root arbitrary.

Public raw read files

Files contain the original full run. Downloads are hosted by ENA.

FileType / bytesMD5
SRR12522753_1.fastq.gzFASTQ / 54253406459bb79237e3dc0f708094a7de521bfd9

Analysis provenance

marker16S
pipelineArchaions v18.8
read_samplingFirst available reads, not random subsampling
input_sha256ca2774cafde902d94a38ad88bc53b27cc88efdcc4359beed7c5e914dbcdc80c7
median_read_length1420.0
reads_in_length_window28078
length_window_bp[1200, 2000]
quality_scoresUnavailable in fetched FASTA; no measured quality filtering
classifier_stats{"marker": "16s", "pipeline": "v18.8", "gpu": "RTX-PRO-6000", "input_path": "volume-fastq-gz", "reads_in": 30000, "reads_skipped": 8230, "elapsed_seconds": 2.613090535000005, "reads_submitted": 30000, "reads_after_filter": 30000, "adapter_scan": {"sampled": 200, "matched": 6, "fraction": 0.03, "adapters_found": {"27F": 1, "BC_FLANK": 5}, "should_trim": false}, "porechop_ran": false, "porechop_reason": "fast-path: no adapter trimming required"}

Full public metadata

All retrieved values are included. Blank source values are displayed as “Not reported”. Expand the sections below to inspect the full record.

ENA run fields (195)
run_accessionSRR12522753
ageNot reported
alignedNot reported
altitudeNot reported
assembly_qualityNot reported
assembly_softwareNot reported
bam_asperaNot reported
bam_bytesNot reported
bam_file_roleNot reported
bam_ftpNot reported
bam_galaxyNot reported
bam_md5Not reported
base_count516216992
binning_softwareNot reported
bio_materialNot reported
bisulfite_protocolNot reported
broad_scale_environmental_contextNot reported
broker_nameNot reported
cage_protocolNot reported
cell_lineNot reported
cell_typeNot reported
center_nameSUB7951364
checklistNot reported
chip_ab_providerNot reported
chip_protocolNot reported
chip_targetNot reported
collected_byNot reported
collection_datenot applicable
collection_date_endNot reported
collection_date_startNot reported
completeness_scoreNot reported
contamination_scoreNot reported
control_experimentNot reported
countryUnited Arab Emirates
cultivarNot reported
culture_collectionNot reported
datahubdcc_metagenome
depthNot reported
descriptionMinION sequencing: metagenomic
dev_stageNot reported
diseaseNot reported
dnase_protocolNot reported
ecotypeNot reported
elevationNot reported
environment_biomeNot reported
environment_featureNot reported
environment_materialNot reported
environmental_mediumNot reported
environmental_sampleNot reported
experiment_accessionSRX9012861
experiment_aliasSH28-P
experiment_targetNot reported
experiment_titleMinION sequencing: metagenomic
experimental_factorNot reported
experimental_protocolNot reported
extraction_protocolNot reported
faang_library_selectionNot reported
fastq_asperafasp.sra.ebi.ac.uk:/vol1/fastq/SRR125/053/SRR12522753/SRR12522753_1.fastq.gz
fastq_bytes542534064
fastq_file_roleGENERATED_FILE
fastq_ftpftp.sra.ebi.ac.uk/vol1/fastq/SRR125/053/SRR12522753/SRR12522753_1.fastq.gz
fastq_galaxyftp.sra.ebi.ac.uk/vol1/fastq/SRR125/053/SRR12522753/SRR12522753_1.fastq.gz
fastq_md559bb79237e3dc0f708094a7de521bfd9
file_locationNot reported
first_created2021-10-01
first_public2021-10-01
germlineNot reported
hi_c_protocolNot reported
hostHomo sapiens
host_body_siteNot reported
host_genotypeNot reported
host_gravidityNot reported
host_growth_conditionsNot reported
host_phenotypeNot reported
host_scientific_nameHomo sapiens
host_sexNot reported
host_statusNot reported
host_tax_id9606
identified_byNot reported
instrument_modelMinION
instrument_platformOXFORD_NANOPORE
investigation_typeNot reported
isolatemixed1
isolation_sourceoral
last_updated2021-10-01
latNot reported
library_construction_protocolNot reported
library_gen_protocolNot reported
library_layoutSINGLE
library_max_fragment_sizeNot reported
library_min_fragment_sizeNot reported
library_nameSH28-P
library_pcr_isolation_protocolNot reported
library_prep_dateNot reported
library_prep_date_formatNot reported
library_prep_latitudeNot reported
library_prep_locationNot reported
library_prep_longitudeNot reported
library_selectionPCR
library_sourceGENOMIC
library_strategyAMPLICON
local_environmental_contextNot reported
locationNot reported
location_endNot reported
location_startNot reported
lonNot reported
marine_regionNot reported
mating_typeNot reported
ncbi_reporting_standardMetagenome or environmental
nominal_lengthNot reported
nominal_sdevNot reported
pcr_isolation_protocolNot reported
phNot reported
project_nameNot reported
protocol_labelNot reported
read_count367794
read_strandNot reported
restriction_enzymeNot reported
restriction_enzyme_target_sequenceNot reported
restriction_siteNot reported
rna_integrity_numNot reported
rna_prep_3_protocolNot reported
rna_prep_5_protocolNot reported
rna_purity_230_ratioNot reported
rna_purity_280_ratioNot reported
rt_prep_protocolNot reported
run_aliasSH28.fastq
run_dateNot reported
salinityNot reported
sample_accessionSAMN15887585
sample_aliasSH28
sample_capture_statusNot reported
sample_collectionNot reported
sample_descriptionMetagenome or environmental sample from human oral metagenome
sample_materialNot reported
sample_prep_intervalNot reported
sample_prep_interval_unitsNot reported
sample_storageNot reported
sample_storage_processingNot reported
sample_titleMetagenome or environmental sample from human oral metagenome
sampling_campaignNot reported
sampling_platformNot reported
sampling_siteNot reported
scientific_namehuman oral metagenome
secondary_projectNot reported
secondary_sample_accessionSRS7263873
secondary_study_accessionSRP278853
sequencing_dateNot reported
sequencing_date_formatNot reported
sequencing_locationNot reported
sequencing_longitudeNot reported
sequencing_methodNot reported
sequencing_primer_catalogNot reported
sequencing_primer_lotNot reported
sequencing_primer_providerNot reported
serotypeNot reported
serovarNot reported
sexNot reported
specimen_voucherNot reported
sra_asperaNot reported
sra_bytesNot reported
sra_file_roleNot reported
sra_ftpNot reported
sra_galaxyNot reported
sra_md5Not reported
statuspublic
strainmixed
study_accessionPRJNA658726
study_aliasPRJNA658726
study_titleThe impact of smoking different tobacco types on the subgingival microbiome and periodontal health: a pilot study
sub_speciesNot reported
sub_strainNot reported
submission_accessionSRA1117712
submission_toolNot reported
submitted_asperaNot reported
submitted_bytesNot reported
submitted_file_roleNot reported
submitted_formatNot reported
submitted_ftpNot reported
submitted_galaxyNot reported
submitted_host_sexNot reported
submitted_md5Not reported
submitted_read_typeNot reported
surveillance_targetNot reported
tagNot reported
target_geneNot reported
tax_id447426
tax_lineage1;2787823;12908;408169;410656;447426
taxonomic_classificationNot reported
taxonomic_identity_markerNot reported
temperatureNot reported
tissue_libNot reported
tissue_typeNot reported
transposase_protocolNot reported
varietyNot reported
BioSample record and attributes
aliasSH28
titleMetagenome or environmental sample from human oral metagenome
descriptionNot reported
strainmixed
collection_datenot applicable
INSDC secondary accessionSRS7263873
aliquots2
organismhuman oral metagenome
sample_typeSubgingival
isolatemixed1
NCBI submission packageMetagenome.environmental.1.0
periodontitisno-mild
smokingS-Shisha
hostHomo sapiens
geo_loc_nameUnited Arab Emirates
lat_lonmissing
isolation_sourceoral
BioSampleModelMetagenome or environmental
ENA-FIRST-PUBLIC2021-09-30
ENA-LAST-UPDATE2021-09-30
Curated cohort annotations and QC
runSRR12522753
sampleSAMN15887585
studyPRJNA658726
titleMetagenome or environmental sample from human oral metagenome
aliasSH28
siteSubgingival
subjectNot reported
phenotypeNot reported
sexNot reported
smokingS-Shisha
periodontitisno-mild
time_pointNot reported
negative_controlFalse
lab_modelFalse
statecomplete
reads_input30000
species_reported83
human_primaryTrue

Model-based taxonomy; research use. This report does not provide a diagnosis or a validated health score.