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archAIons · MetaBiome Project · Oral · Public research data

SRR12522749

Metagenome or environmental sample from human oral metagenome

BioSample: SAMN15887589 · Study: PRJNA658726 · Body site: Subgingival

Interactive report · NCBI SRA · ENA

64Reported species
19Reported genera

Taxonomic abundance

Relative abundance is normalized among reported taxa. No reported taxa does not establish absence of organisms.

SpeciesGenusPhylumAbundance
Fusobacterium nucleatumFusobacteriumFusobacteriota16.33993%
Streptococcus mitisStreptococcusBacillota15.58399%
Fusobacterium perfoetensFusobacteriumFusobacteriota9.35411%
Streptococcus downeiStreptococcusBacillota5.39976%
Campylobacter gracilisCampylobacterPseudomonadota4.69630%
Fusobacterium russiiFusobacteriumFusobacteriota3.88211%
Streptococcus salivariusStreptococcusBacillota3.27927%
Eubacterium ramulusEubacteriumBacillota3.14708%
Campylobacter hyointestinalisCampylobacterPseudomonadota3.10206%
Veillonella parvulaVeillonellaBacillota3.02280%
Streptococcus pneumoniaeStreptococcusBacillota2.57047%
Filifactor alocisFilifactorBacillota2.25293%
Campylobacter sputorumCampylobacterPseudomonadota1.70914%
Fusobacterium simiaeFusobacteriumFusobacteriota1.51083%
Filifactor villosusFilifactorBacillota1.30919%
Capnocytophaga canisCapnocytophagaBacteroidota1.29033%
Dialister invisusDialisterBacillota1.11871%
Haemophilus parainfluenzaeHaemophilusPseudomonadota0.93132%
Neisseria flavescensNeisseriaPseudomonadota0.87216%
Eubacterium limosumEubacteriumBacillota0.84813%
Leptotrichia hongkongensisLeptotrichiaFusobacteriota0.82057%
Parvimonas micraParvimonasBacillota0.82054%
Campylobacter showaeCampylobacterPseudomonadota0.80004%
Peptostreptococcus anaerobiusPeptostreptococcusBacillota0.79834%
Pseudoleptotrichia goodfellowiiPseudoleptotrichiaFusobacteriota0.76066%
Mogibacterium pumilumMogibacteriumBacillota0.70153%
Streptococcus sanguinisStreptococcusBacillota0.67174%
Streptococcus infantisStreptococcusBacillota0.66355%
Fusobacterium periodonticumFusobacteriumFusobacteriota0.64270%
Gemella palaticanisGemellaBacillota0.62881%
Lachnoanaerobaculum saburreumLachnoanaerobaculumBacillota0.62008%
Mogibacterium timidumMogibacteriumBacillota0.54361%
Streptococcus pyogenesStreptococcusBacillota0.52244%
Streptococcus oralisStreptococcusBacillota0.50052%
Streptococcus anginosusStreptococcusBacillota0.45201%
Streptococcus intermediusStreptococcusBacillota0.44462%
Gemella haemolysansGemellaBacillota0.42995%
Veillonella infantiumVeillonellaBacillota0.37696%
Granulicatella adiacensGranulicatellaBacillota0.37057%
Mogibacterium diversumMogibacteriumBacillota0.34847%
Haemophilus haemolyticusHaemophilusPseudomonadota0.34581%
Dialister hominisDialisterBacillota0.33859%
Streptococcus cristatusStreptococcusBacillota0.33728%
Lachnoanaerobaculum oraleLachnoanaerobaculumBacillota0.33723%
Capnocytophaga granulosaCapnocytophagaBacteroidota0.33147%
Prevotella ruminicolaPrevotellaBacteroidota0.31101%
Neisseria macacaeNeisseriaPseudomonadota0.30318%
Eubacterium xylanophilumEubacteriumBacillota0.29930%
Mogibacterium vescumMogibacteriumBacillota0.27719%
Streptococcus gordoniiStreptococcusBacillota0.26931%
Leptotrichia shahiiLeptotrichiaFusobacteriota0.23818%
Neisseria mucosaNeisseriaPseudomonadota0.23413%
Lachnoanaerobaculum umeaenseLachnoanaerobaculumBacillota0.23372%
Capnocytophaga gingivalisCapnocytophagaBacteroidota0.22155%
Streptococcus alactolyticusStreptococcusBacillota0.20633%
Campylobacter concisusCampylobacterPseudomonadota0.20548%
Eubacterium pyruvativoransEubacteriumBacillota0.19195%
Streptococcus australisStreptococcusBacillota0.18566%
Streptococcus gallolyticusStreptococcusBacillota0.17788%
Peptostreptococcus stomatisPeptostreptococcusBacillota0.17442%
Streptococcus marmotaeStreptococcusBacillota0.17281%
Capnocytophaga ochraceaCapnocytophagaBacteroidota0.16829%
Campylobacter rectusCampylobacterPseudomonadota0.16099%
Streptococcus periodonticumStreptococcusBacillota0.13990%

Reference-based 16S phylogeny

63 of 64 reported species have an eligible reference. MAFFT alignment and FastTree GTR+CAT tree, pruned to this sample. This is not a phylogeny inferred from sample reads or ASVs. Root placement is arbitrary; branch lengths are substitutions per site.

Streptococcus periodonticum0.14%Streptococcus anginosus0.45%Streptococcus intermedius0.44%Streptococcus cristatus0.34%Streptococcus infantis0.66%Streptococcus oralis0.50%Streptococcus mitis15.58%Streptococcus pneumoniae2.57%Streptococcus australis0.19%Streptococcus marmotae0.17%Streptococcus gordonii0.27%Streptococcus sanguinis0.67%Streptococcus alactolyticus0.21%Streptococcus gallolyticus0.18%Streptococcus pyogenes0.52%Granulicatella adiacens0.37%Gemella palaticanis0.63%Gemella haemolysans0.43%Veillonella infantium0.38%Veillonella parvula3.02%Dialister invisus1.12%Dialister hominis0.34%Eubacterium xylanophilum0.30%Eubacterium ramulus3.15%Lachnoanaerobaculum umeaense0.23%Lachnoanaerobaculum orale0.34%Lachnoanaerobaculum saburreum0.62%Eubacterium limosum0.85%Parvimonas micra0.82%Peptostreptococcus anaerobius0.80%Peptostreptococcus stomatis0.17%Filifactor alocis2.25%Filifactor villosus1.31%Mogibacterium pumilum0.70%Mogibacterium timidum0.54%Mogibacterium diversum0.35%Mogibacterium vescum0.28%Eubacterium pyruvativorans0.19%Fusobacterium perfoetens9.35%Fusobacterium russii3.88%Fusobacterium periodonticum0.64%Fusobacterium nucleatum16.34%Fusobacterium simiae1.51%Pseudoleptotrichia goodfellowii0.76%Leptotrichia hongkongensis0.82%Leptotrichia shahii0.24%Campylobacter sputorum1.71%Campylobacter gracilis4.70%Campylobacter rectus0.16%Campylobacter showae0.80%Campylobacter concisus0.21%Campylobacter hyointestinalis3.10%Neisseria mucosa0.23%Neisseria macacae0.30%Neisseria flavescens0.87%Haemophilus parainfluenzae0.93%Haemophilus haemolyticus0.35%Prevotella ruminicola0.31%Capnocytophaga gingivalis0.22%Capnocytophaga granulosa0.33%Capnocytophaga ochracea0.17%Streptococcus downei5.40%Streptococcus salivarius3.28%Branch length scale: 1.0175 substitutions/site across the 500-unit horizontal axis. Root arbitrary.

Public raw read files

Files contain the original full run. Downloads are hosted by ENA.

FileType / bytesMD5
SRR12522749_1.fastq.gzFASTQ / 6097072950e8fdf1a1573c20c39747c25a3c219b6

Analysis provenance

marker16S
pipelineArchaions v18.8
read_samplingFirst available reads, not random subsampling
input_sha25604cb6f7e37e72711261432b3d0b3870306d00fdbc790186108b0300440c69ff8
median_read_length1421.0
reads_in_length_window19095
length_window_bp[1200, 2000]
quality_scoresUnavailable in fetched FASTA; no measured quality filtering
classifier_stats{"marker": "16s", "pipeline": "v18.8", "gpu": "RTX-PRO-6000", "input_path": "volume-fastq-gz", "reads_in": 20000, "reads_skipped": 4686, "elapsed_seconds": 2.869111276, "reads_submitted": 20000, "reads_after_filter": 20000, "adapter_scan": {"sampled": 200, "matched": 2, "fraction": 0.01, "adapters_found": {"BC_FLANK": 1, "27F": 1}, "should_trim": false}, "porechop_ran": false, "porechop_reason": "fast-path: no adapter trimming required"}

Full public metadata

All retrieved values are included. Blank source values are displayed as “Not reported”. Expand the sections below to inspect the full record.

ENA run fields (195)
run_accessionSRR12522749
ageNot reported
alignedNot reported
altitudeNot reported
assembly_qualityNot reported
assembly_softwareNot reported
bam_asperaNot reported
bam_bytesNot reported
bam_file_roleNot reported
bam_ftpNot reported
bam_galaxyNot reported
bam_md5Not reported
base_count584873615
binning_softwareNot reported
bio_materialNot reported
bisulfite_protocolNot reported
broad_scale_environmental_contextNot reported
broker_nameNot reported
cage_protocolNot reported
cell_lineNot reported
cell_typeNot reported
center_nameSUB7951364
checklistNot reported
chip_ab_providerNot reported
chip_protocolNot reported
chip_targetNot reported
collected_byNot reported
collection_datenot applicable
collection_date_endNot reported
collection_date_startNot reported
completeness_scoreNot reported
contamination_scoreNot reported
control_experimentNot reported
countryUnited Arab Emirates
cultivarNot reported
culture_collectionNot reported
datahubdcc_metagenome
depthNot reported
descriptionMinION sequencing: metagenomic
dev_stageNot reported
diseaseNot reported
dnase_protocolNot reported
ecotypeNot reported
elevationNot reported
environment_biomeNot reported
environment_featureNot reported
environment_materialNot reported
environmental_mediumNot reported
environmental_sampleNot reported
experiment_accessionSRX9012865
experiment_aliasSH60-P
experiment_targetNot reported
experiment_titleMinION sequencing: metagenomic
experimental_factorNot reported
experimental_protocolNot reported
extraction_protocolNot reported
faang_library_selectionNot reported
fastq_asperafasp.sra.ebi.ac.uk:/vol1/fastq/SRR125/049/SRR12522749/SRR12522749_1.fastq.gz
fastq_bytes609707295
fastq_file_roleGENERATED_FILE
fastq_ftpftp.sra.ebi.ac.uk/vol1/fastq/SRR125/049/SRR12522749/SRR12522749_1.fastq.gz
fastq_galaxyftp.sra.ebi.ac.uk/vol1/fastq/SRR125/049/SRR12522749/SRR12522749_1.fastq.gz
fastq_md50e8fdf1a1573c20c39747c25a3c219b6
file_locationNot reported
first_created2021-10-01
first_public2021-10-01
germlineNot reported
hi_c_protocolNot reported
hostHomo sapiens
host_body_siteNot reported
host_genotypeNot reported
host_gravidityNot reported
host_growth_conditionsNot reported
host_phenotypeNot reported
host_scientific_nameHomo sapiens
host_sexNot reported
host_statusNot reported
host_tax_id9606
identified_byNot reported
instrument_modelMinION
instrument_platformOXFORD_NANOPORE
investigation_typeNot reported
isolatemixed1
isolation_sourceoral
last_updated2021-10-01
latNot reported
library_construction_protocolNot reported
library_gen_protocolNot reported
library_layoutSINGLE
library_max_fragment_sizeNot reported
library_min_fragment_sizeNot reported
library_nameSH60-P
library_pcr_isolation_protocolNot reported
library_prep_dateNot reported
library_prep_date_formatNot reported
library_prep_latitudeNot reported
library_prep_locationNot reported
library_prep_longitudeNot reported
library_selectionPCR
library_sourceGENOMIC
library_strategyAMPLICON
local_environmental_contextNot reported
locationNot reported
location_endNot reported
location_startNot reported
lonNot reported
marine_regionNot reported
mating_typeNot reported
ncbi_reporting_standardMetagenome or environmental
nominal_lengthNot reported
nominal_sdevNot reported
pcr_isolation_protocolNot reported
phNot reported
project_nameNot reported
protocol_labelNot reported
read_count418805
read_strandNot reported
restriction_enzymeNot reported
restriction_enzyme_target_sequenceNot reported
restriction_siteNot reported
rna_integrity_numNot reported
rna_prep_3_protocolNot reported
rna_prep_5_protocolNot reported
rna_purity_230_ratioNot reported
rna_purity_280_ratioNot reported
rt_prep_protocolNot reported
run_aliasSH60.fastq
run_dateNot reported
salinityNot reported
sample_accessionSAMN15887589
sample_aliasSH60
sample_capture_statusNot reported
sample_collectionNot reported
sample_descriptionMetagenome or environmental sample from human oral metagenome
sample_materialNot reported
sample_prep_intervalNot reported
sample_prep_interval_unitsNot reported
sample_storageNot reported
sample_storage_processingNot reported
sample_titleMetagenome or environmental sample from human oral metagenome
sampling_campaignNot reported
sampling_platformNot reported
sampling_siteNot reported
scientific_namehuman oral metagenome
secondary_projectNot reported
secondary_sample_accessionSRS7263876
secondary_study_accessionSRP278853
sequencing_dateNot reported
sequencing_date_formatNot reported
sequencing_locationNot reported
sequencing_longitudeNot reported
sequencing_methodNot reported
sequencing_primer_catalogNot reported
sequencing_primer_lotNot reported
sequencing_primer_providerNot reported
serotypeNot reported
serovarNot reported
sexNot reported
specimen_voucherNot reported
sra_asperaNot reported
sra_bytesNot reported
sra_file_roleNot reported
sra_ftpNot reported
sra_galaxyNot reported
sra_md5Not reported
statuspublic
strainmixed
study_accessionPRJNA658726
study_aliasPRJNA658726
study_titleThe impact of smoking different tobacco types on the subgingival microbiome and periodontal health: a pilot study
sub_speciesNot reported
sub_strainNot reported
submission_accessionSRA1117712
submission_toolNot reported
submitted_asperaNot reported
submitted_bytesNot reported
submitted_file_roleNot reported
submitted_formatNot reported
submitted_ftpNot reported
submitted_galaxyNot reported
submitted_host_sexNot reported
submitted_md5Not reported
submitted_read_typeNot reported
surveillance_targetNot reported
tagNot reported
target_geneNot reported
tax_id447426
tax_lineage1;2787823;12908;408169;410656;447426
taxonomic_classificationNot reported
taxonomic_identity_markerNot reported
temperatureNot reported
tissue_libNot reported
tissue_typeNot reported
transposase_protocolNot reported
varietyNot reported
BioSample record and attributes
aliasSH60
titleMetagenome or environmental sample from human oral metagenome
descriptionNot reported
strainmixed
collection_datenot applicable
INSDC secondary accessionSRS7263876
aliquots6
organismhuman oral metagenome
sample_typeSubgingival
isolatemixed1
NCBI submission packageMetagenome.environmental.1.0
periodontitisno-mild
smokingS-Shisha
hostHomo sapiens
geo_loc_nameUnited Arab Emirates
lat_lonmissing
isolation_sourceoral
BioSampleModelMetagenome or environmental
ENA-FIRST-PUBLIC2021-09-30
ENA-LAST-UPDATE2021-09-30
Curated cohort annotations and QC
runSRR12522749
sampleSAMN15887589
studyPRJNA658726
titleMetagenome or environmental sample from human oral metagenome
aliasSH60
siteSubgingival
subjectNot reported
phenotypeNot reported
sexNot reported
smokingS-Shisha
periodontitisno-mild
time_pointNot reported
negative_controlFalse
lab_modelFalse
statecomplete
reads_input20000
species_reported64
human_primaryTrue

Model-based taxonomy; research use. This report does not provide a diagnosis or a validated health score.