archAIons
archAIons · MetaBiome Project · Oral · Public research data

SRR12522745

Metagenome or environmental sample from human oral metagenome

BioSample: SAMN15887593 · Study: PRJNA658726 · Body site: Subgingival

Interactive report · NCBI SRA · ENA

72Reported species
19Reported genera

Taxonomic abundance

Relative abundance is normalized among reported taxa. No reported taxa does not establish absence of organisms.

SpeciesGenusPhylumAbundance
Fusobacterium nucleatumFusobacteriumFusobacteriota9.88306%
Streptococcus mitisStreptococcusBacillota9.01272%
Filifactor alocisFilifactorBacillota8.30485%
Eubacterium ramulusEubacteriumBacillota7.27138%
Fusobacterium perfoetensFusobacteriumFusobacteriota6.22400%
Veillonella parvulaVeillonellaBacillota4.84650%
Selenomonas flueggeiSelenomonasBacillota4.11734%
Streptococcus downeiStreptococcusBacillota4.10956%
Filifactor villosusFilifactorBacillota3.95344%
Dialister invisusDialisterBacillota3.14456%
Parvimonas micraParvimonasBacillota2.92503%
Mogibacterium timidumMogibacteriumBacillota2.80343%
Fusobacterium russiiFusobacteriumFusobacteriota2.74791%
Selenomonas sputigenaSelenomonasBacillota2.39399%
Peptostreptococcus anaerobiusPeptostreptococcusBacillota2.24799%
Streptococcus salivariusStreptococcusBacillota1.70959%
Gemella palaticanisGemellaBacillota1.31804%
Eubacterium limosumEubacteriumBacillota1.25961%
Streptococcus pneumoniaeStreptococcusBacillota1.24207%
Mogibacterium pumilumMogibacteriumBacillota1.16883%
Tannerella forsythiaTannerellaBacteroidota1.04187%
Leptotrichia hongkongensisLeptotrichiaFusobacteriota0.93865%
Selenomonas dianaeSelenomonasBacillota0.87017%
Campylobacter showaeCampylobacterPseudomonadota0.77118%
Campylobacter gracilisCampylobacterPseudomonadota0.68312%
Dialister hominisDialisterBacillota0.66628%
Fusobacterium simiaeFusobacteriumFusobacteriota0.66437%
Campylobacter hyointestinalisCampylobacterPseudomonadota0.61602%
Streptococcus infantisStreptococcusBacillota0.60580%
Streptococcus cristatusStreptococcusBacillota0.57919%
Gemella haemolysansGemellaBacillota0.55754%
Campylobacter sputorumCampylobacterPseudomonadota0.55128%
Capnocytophaga canisCapnocytophagaBacteroidota0.53806%
Neisseria flavescensNeisseriaPseudomonadota0.53658%
Mogibacterium vescumMogibacteriumBacillota0.47225%
Veillonella infantiumVeillonellaBacillota0.46074%
Mogibacterium diversumMogibacteriumBacillota0.45579%
Streptococcus oralisStreptococcusBacillota0.42227%
Aggregatibacter segnisAggregatibacterPseudomonadota0.40894%
Streptococcus pyogenesStreptococcusBacillota0.40630%
Prevotella ruminicolaPrevotellaBacteroidota0.38935%
Fusobacterium periodonticumFusobacteriumFusobacteriota0.36137%
Selenomonas ruminantiumSelenomonasBacillota0.34474%
Streptococcus anginosusStreptococcusBacillota0.33815%
Eubacterium xylanophilumEubacteriumBacillota0.31901%
Peptostreptococcus stomatisPeptostreptococcusBacillota0.29862%
Prevotella orisPrevotellaBacteroidota0.28567%
Eubacterium callanderiEubacteriumBacillota0.26943%
Eubacterium multiformeEubacteriumBacillota0.26533%
Aggregatibacter aphrophilusAggregatibacterPseudomonadota0.25629%
Gemella morbillorumGemellaBacillota0.25229%
Granulicatella adiacensGranulicatellaBacillota0.24950%
Selenomonas infelixSelenomonasBacillota0.24275%
Neisseria macacaeNeisseriaPseudomonadota0.23138%
Leptotrichia shahiiLeptotrichiaFusobacteriota0.22818%
Eubacterium barkeriEubacteriumBacillota0.20423%
Eubacterium pyruvativoransEubacteriumBacillota0.20046%
Veillonella disparVeillonellaBacillota0.19687%
Leptotrichia wadeiLeptotrichiaFusobacteriota0.18801%
Streptococcus gordoniiStreptococcusBacillota0.18171%
Streptococcus suisStreptococcusBacillota0.17015%
Streptococcus alactolyticusStreptococcusBacillota0.16921%
Mogibacterium neglectumMogibacteriumBacillota0.16169%
Dialister pneumosintesDialisterBacillota0.15277%
Veillonella atypicaVeillonellaBacillota0.15162%
Streptococcus australisStreptococcusBacillota0.15132%
Eubacterium ventriosumEubacteriumBacillota0.15011%
Streptococcus sanguinisStreptococcusBacillota0.14191%
Dialister propionicifaciensDialisterBacillota0.13652%
Streptococcus intermediusStreptococcusBacillota0.13194%
Streptococcus constellatusStreptococcusBacillota0.12523%
Selenomonas noxiaSelenomonasBacillota0.12392%

Reference-based 16S phylogeny

71 of 72 reported species have an eligible reference. MAFFT alignment and FastTree GTR+CAT tree, pruned to this sample. This is not a phylogeny inferred from sample reads or ASVs. Root placement is arbitrary; branch lengths are substitutions per site.

Streptococcus anginosus0.34%Streptococcus constellatus0.13%Streptococcus intermedius0.13%Streptococcus cristatus0.58%Streptococcus infantis0.61%Streptococcus oralis0.42%Streptococcus mitis9.01%Streptococcus pneumoniae1.24%Streptococcus australis0.15%Streptococcus gordonii0.18%Streptococcus sanguinis0.14%Streptococcus suis0.17%Streptococcus alactolyticus0.17%Streptococcus pyogenes0.41%Granulicatella adiacens0.25%Gemella palaticanis1.32%Gemella haemolysans0.56%Gemella morbillorum0.25%Veillonella dispar0.20%Veillonella infantium0.46%Veillonella parvula4.85%Veillonella atypica0.15%Dialister invisus3.14%Dialister pneumosintes0.15%Dialister propionicifaciens0.14%Dialister hominis0.67%Selenomonas ruminantium0.34%Selenomonas sputigena2.39%Selenomonas noxia0.12%Selenomonas flueggei4.12%Selenomonas dianae0.87%Selenomonas infelix0.24%Eubacterium xylanophilum0.32%Eubacterium ramulus7.27%Eubacterium ventriosum0.15%Eubacterium multiforme0.27%Eubacterium barkeri0.20%Eubacterium callanderi0.27%Eubacterium limosum1.26%Parvimonas micra2.93%Peptostreptococcus anaerobius2.25%Peptostreptococcus stomatis0.30%Filifactor alocis8.30%Filifactor villosus3.95%Mogibacterium pumilum1.17%Mogibacterium timidum2.80%Mogibacterium neglectum0.16%Mogibacterium diversum0.46%Mogibacterium vescum0.47%Eubacterium pyruvativorans0.20%Fusobacterium perfoetens6.22%Fusobacterium russii2.75%Fusobacterium periodonticum0.36%Fusobacterium nucleatum9.88%Fusobacterium simiae0.66%Leptotrichia hongkongensis0.94%Leptotrichia wadei0.19%Leptotrichia shahii0.23%Campylobacter sputorum0.55%Campylobacter gracilis0.68%Campylobacter showae0.77%Campylobacter hyointestinalis0.62%Neisseria macacae0.23%Neisseria flavescens0.54%Aggregatibacter segnis0.41%Aggregatibacter aphrophilus0.26%Tannerella forsythia1.04%Prevotella oris0.29%Prevotella ruminicola0.39%Streptococcus downei4.11%Streptococcus salivarius1.71%Branch length scale: 1.0175 substitutions/site across the 500-unit horizontal axis. Root arbitrary.

Public raw read files

Files contain the original full run. Downloads are hosted by ENA.

FileType / bytesMD5
SRR12522745_1.fastq.gzFASTQ / 5771803244540ebcec74bb0d395ad3e76aa15e0d3

Analysis provenance

marker16S
pipelineArchaions v18.8
read_samplingFirst available reads, not random subsampling
input_sha25671be0589bd9622e5e10dde816c03baf8a0644f869b5ae6914c570d22d5c2eed2
median_read_length1411.0
reads_in_length_window27950
length_window_bp[1200, 2000]
quality_scoresUnavailable in fetched FASTA; no measured quality filtering
classifier_stats{"marker": "16s", "pipeline": "v18.8", "gpu": "RTX-PRO-6000", "input_path": "volume-fastq-gz", "reads_in": 30000, "reads_skipped": 9397, "elapsed_seconds": 2.7552555510000047, "reads_submitted": 30000, "reads_after_filter": 30000, "adapter_scan": {"sampled": 200, "matched": 3, "fraction": 0.015, "adapters_found": {"BC_FLANK": 2, "27F": 1}, "should_trim": false}, "porechop_ran": false, "porechop_reason": "fast-path: no adapter trimming required"}

Full public metadata

All retrieved values are included. Blank source values are displayed as “Not reported”. Expand the sections below to inspect the full record.

ENA run fields (195)
run_accessionSRR12522745
ageNot reported
alignedNot reported
altitudeNot reported
assembly_qualityNot reported
assembly_softwareNot reported
bam_asperaNot reported
bam_bytesNot reported
bam_file_roleNot reported
bam_ftpNot reported
bam_galaxyNot reported
bam_md5Not reported
base_count549808255
binning_softwareNot reported
bio_materialNot reported
bisulfite_protocolNot reported
broad_scale_environmental_contextNot reported
broker_nameNot reported
cage_protocolNot reported
cell_lineNot reported
cell_typeNot reported
center_nameSUB7951364
checklistNot reported
chip_ab_providerNot reported
chip_protocolNot reported
chip_targetNot reported
collected_byNot reported
collection_datenot applicable
collection_date_endNot reported
collection_date_startNot reported
completeness_scoreNot reported
contamination_scoreNot reported
control_experimentNot reported
countryUnited Arab Emirates
cultivarNot reported
culture_collectionNot reported
datahubdcc_metagenome
depthNot reported
descriptionMinION sequencing: metagenomic
dev_stageNot reported
diseaseNot reported
dnase_protocolNot reported
ecotypeNot reported
elevationNot reported
environment_biomeNot reported
environment_featureNot reported
environment_materialNot reported
environmental_mediumNot reported
environmental_sampleNot reported
experiment_accessionSRX9012869
experiment_aliasSH70-P
experiment_targetNot reported
experiment_titleMinION sequencing: metagenomic
experimental_factorNot reported
experimental_protocolNot reported
extraction_protocolNot reported
faang_library_selectionNot reported
fastq_asperafasp.sra.ebi.ac.uk:/vol1/fastq/SRR125/045/SRR12522745/SRR12522745_1.fastq.gz
fastq_bytes577180324
fastq_file_roleGENERATED_FILE
fastq_ftpftp.sra.ebi.ac.uk/vol1/fastq/SRR125/045/SRR12522745/SRR12522745_1.fastq.gz
fastq_galaxyftp.sra.ebi.ac.uk/vol1/fastq/SRR125/045/SRR12522745/SRR12522745_1.fastq.gz
fastq_md54540ebcec74bb0d395ad3e76aa15e0d3
file_locationNot reported
first_created2021-10-01
first_public2021-10-01
germlineNot reported
hi_c_protocolNot reported
hostHomo sapiens
host_body_siteNot reported
host_genotypeNot reported
host_gravidityNot reported
host_growth_conditionsNot reported
host_phenotypeNot reported
host_scientific_nameHomo sapiens
host_sexNot reported
host_statusNot reported
host_tax_id9606
identified_byNot reported
instrument_modelMinION
instrument_platformOXFORD_NANOPORE
investigation_typeNot reported
isolatemixed1
isolation_sourceoral
last_updated2021-10-01
latNot reported
library_construction_protocolNot reported
library_gen_protocolNot reported
library_layoutSINGLE
library_max_fragment_sizeNot reported
library_min_fragment_sizeNot reported
library_nameSH70-P
library_pcr_isolation_protocolNot reported
library_prep_dateNot reported
library_prep_date_formatNot reported
library_prep_latitudeNot reported
library_prep_locationNot reported
library_prep_longitudeNot reported
library_selectionPCR
library_sourceGENOMIC
library_strategyAMPLICON
local_environmental_contextNot reported
locationNot reported
location_endNot reported
location_startNot reported
lonNot reported
marine_regionNot reported
mating_typeNot reported
ncbi_reporting_standardMetagenome or environmental
nominal_lengthNot reported
nominal_sdevNot reported
pcr_isolation_protocolNot reported
phNot reported
project_nameNot reported
protocol_labelNot reported
read_count394405
read_strandNot reported
restriction_enzymeNot reported
restriction_enzyme_target_sequenceNot reported
restriction_siteNot reported
rna_integrity_numNot reported
rna_prep_3_protocolNot reported
rna_prep_5_protocolNot reported
rna_purity_230_ratioNot reported
rna_purity_280_ratioNot reported
rt_prep_protocolNot reported
run_aliasSH70.fastq
run_dateNot reported
salinityNot reported
sample_accessionSAMN15887593
sample_aliasSH70
sample_capture_statusNot reported
sample_collectionNot reported
sample_descriptionMetagenome or environmental sample from human oral metagenome
sample_materialNot reported
sample_prep_intervalNot reported
sample_prep_interval_unitsNot reported
sample_storageNot reported
sample_storage_processingNot reported
sample_titleMetagenome or environmental sample from human oral metagenome
sampling_campaignNot reported
sampling_platformNot reported
sampling_siteNot reported
scientific_namehuman oral metagenome
secondary_projectNot reported
secondary_sample_accessionSRS7263881
secondary_study_accessionSRP278853
sequencing_dateNot reported
sequencing_date_formatNot reported
sequencing_locationNot reported
sequencing_longitudeNot reported
sequencing_methodNot reported
sequencing_primer_catalogNot reported
sequencing_primer_lotNot reported
sequencing_primer_providerNot reported
serotypeNot reported
serovarNot reported
sexNot reported
specimen_voucherNot reported
sra_asperaNot reported
sra_bytesNot reported
sra_file_roleNot reported
sra_ftpNot reported
sra_galaxyNot reported
sra_md5Not reported
statuspublic
strainmixed
study_accessionPRJNA658726
study_aliasPRJNA658726
study_titleThe impact of smoking different tobacco types on the subgingival microbiome and periodontal health: a pilot study
sub_speciesNot reported
sub_strainNot reported
submission_accessionSRA1117712
submission_toolNot reported
submitted_asperaNot reported
submitted_bytesNot reported
submitted_file_roleNot reported
submitted_formatNot reported
submitted_ftpNot reported
submitted_galaxyNot reported
submitted_host_sexNot reported
submitted_md5Not reported
submitted_read_typeNot reported
surveillance_targetNot reported
tagNot reported
target_geneNot reported
tax_id447426
tax_lineage1;2787823;12908;408169;410656;447426
taxonomic_classificationNot reported
taxonomic_identity_markerNot reported
temperatureNot reported
tissue_libNot reported
tissue_typeNot reported
transposase_protocolNot reported
varietyNot reported
BioSample record and attributes
aliasSH70
titleMetagenome or environmental sample from human oral metagenome
descriptionNot reported
strainmixed
collection_datenot applicable
INSDC secondary accessionSRS7263881
aliquots10
organismhuman oral metagenome
sample_typeSubgingival
isolatemixed1
NCBI submission packageMetagenome.environmental.1.0
periodontitisno-mild
smokingS-Shisha
hostHomo sapiens
geo_loc_nameUnited Arab Emirates
lat_lonmissing
isolation_sourceoral
BioSampleModelMetagenome or environmental
ENA-FIRST-PUBLIC2021-09-30
ENA-LAST-UPDATE2021-09-30
Curated cohort annotations and QC
runSRR12522745
sampleSAMN15887593
studyPRJNA658726
titleMetagenome or environmental sample from human oral metagenome
aliasSH70
siteSubgingival
subjectNot reported
phenotypeNot reported
sexNot reported
smokingS-Shisha
periodontitisno-mild
time_pointNot reported
negative_controlFalse
lab_modelFalse
statecomplete
reads_input30000
species_reported72
human_primaryTrue

Model-based taxonomy; research use. This report does not provide a diagnosis or a validated health score.