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SRR12522741

Metagenome or environmental sample from human oral metagenome

BioSample: SAMN15887596 · Study: PRJNA658726 · Body site: Subgingival

Interactive report · NCBI SRA · ENA

55Reported species
18Reported genera

Taxonomic abundance

Relative abundance is normalized among reported taxa. No reported taxa does not establish absence of organisms.

SpeciesGenusPhylumAbundance
Streptococcus mitisStreptococcusBacillota18.58120%
Streptococcus downeiStreptococcusBacillota7.77215%
Selenomonas flueggeiSelenomonasBacillota7.58927%
Veillonella parvulaVeillonellaBacillota7.33657%
Fusobacterium nucleatumFusobacteriumFusobacteriota4.86129%
Streptococcus salivariusStreptococcusBacillota4.61171%
Fusobacterium perfoetensFusobacteriumFusobacteriota3.19493%
Streptococcus pneumoniaeStreptococcusBacillota2.57068%
Prevotella ruminicolaPrevotellaBacteroidota2.40743%
Eubacterium ramulusEubacteriumBacillota2.25829%
Lachnoanaerobaculum saburreumLachnoanaerobaculumBacillota2.25375%
Parvimonas micraParvimonasBacillota2.04143%
Dialister invisusDialisterBacillota1.83542%
Leptotrichia hongkongensisLeptotrichiaFusobacteriota1.80573%
Campylobacter gracilisCampylobacterPseudomonadota1.61132%
Prevotella denticolaPrevotellaBacteroidota1.52238%
Selenomonas sputigenaSelenomonasBacillota1.42247%
Campylobacter hyointestinalisCampylobacterPseudomonadota1.21538%
Capnocytophaga canisCapnocytophagaBacteroidota1.21043%
Streptococcus cristatusStreptococcusBacillota1.20217%
Fusobacterium russiiFusobacteriumFusobacteriota1.15340%
Selenomonas dianaeSelenomonasBacillota1.11371%
Lachnoanaerobaculum oraleLachnoanaerobaculumBacillota1.07330%
Granulicatella adiacensGranulicatellaBacillota1.02421%
Campylobacter sputorumCampylobacterPseudomonadota0.99042%
Veillonella infantiumVeillonellaBacillota0.97205%
Filifactor alocisFilifactorBacillota0.97107%
Streptococcus oralisStreptococcusBacillota0.91689%
Mogibacterium pumilumMogibacteriumBacillota0.79517%
Mogibacterium timidumMogibacteriumBacillota0.76438%
Streptococcus pyogenesStreptococcusBacillota0.74160%
Lachnoanaerobaculum umeaenseLachnoanaerobaculumBacillota0.70992%
Leptotrichia wadeiLeptotrichiaFusobacteriota0.68987%
Streptococcus gordoniiStreptococcusBacillota0.65160%
Streptococcus infantisStreptococcusBacillota0.64058%
Selenomonas noxiaSelenomonasBacillota0.63020%
Streptococcus anginosusStreptococcusBacillota0.62650%
Streptococcus intermediusStreptococcusBacillota0.62592%
Prevotella nigrescensPrevotellaBacteroidota0.60646%
Gemella palaticanisGemellaBacillota0.55866%
Filifactor villosusFilifactorBacillota0.55860%
Prevotella orisPrevotellaBacteroidota0.54469%
Selenomonas ruminantiumSelenomonasBacillota0.52014%
Veillonella disparVeillonellaBacillota0.51040%
Dialister hominisDialisterBacillota0.48711%
Campylobacter showaeCampylobacterPseudomonadota0.47926%
Prevotella intermediaPrevotellaBacteroidota0.45243%
Leptotrichia shahiiLeptotrichiaFusobacteriota0.44401%
Capnocytophaga gingivalisCapnocytophagaBacteroidota0.43895%
Fusobacterium simiaeFusobacteriumFusobacteriota0.42059%
Selenomonas infelixSelenomonasBacillota0.35467%
Porphyromonas endodontalisPorphyromonasBacteroidota0.34581%
Streptococcus sanguinisStreptococcusBacillota0.32475%
Porphyromonas circumdentariaPorphyromonasBacteroidota0.31926%
Haemophilus parainfluenzaeHaemophilusPseudomonadota0.23943%

Reference-based 16S phylogeny

54 of 55 reported species have an eligible reference. MAFFT alignment and FastTree GTR+CAT tree, pruned to this sample. This is not a phylogeny inferred from sample reads or ASVs. Root placement is arbitrary; branch lengths are substitutions per site.

Streptococcus anginosus0.63%Streptococcus intermedius0.63%Streptococcus cristatus1.20%Streptococcus infantis0.64%Streptococcus oralis0.92%Streptococcus mitis18.58%Streptococcus pneumoniae2.57%Streptococcus gordonii0.65%Streptococcus sanguinis0.32%Streptococcus pyogenes0.74%Granulicatella adiacens1.02%Gemella palaticanis0.56%Veillonella dispar0.51%Veillonella infantium0.97%Veillonella parvula7.34%Dialister invisus1.84%Dialister hominis0.49%Selenomonas ruminantium0.52%Selenomonas sputigena1.42%Selenomonas noxia0.63%Selenomonas flueggei7.59%Selenomonas dianae1.11%Selenomonas infelix0.35%Eubacterium ramulus2.26%Lachnoanaerobaculum umeaense0.71%Lachnoanaerobaculum orale1.07%Lachnoanaerobaculum saburreum2.25%Parvimonas micra2.04%Filifactor alocis0.97%Filifactor villosus0.56%Mogibacterium pumilum0.80%Mogibacterium timidum0.76%Fusobacterium perfoetens3.19%Fusobacterium russii1.15%Fusobacterium nucleatum4.86%Fusobacterium simiae0.42%Leptotrichia hongkongensis1.81%Leptotrichia wadei0.69%Leptotrichia shahii0.44%Campylobacter sputorum0.99%Campylobacter gracilis1.61%Campylobacter showae0.48%Campylobacter hyointestinalis1.22%Haemophilus parainfluenzae0.24%Porphyromonas endodontalis0.35%Porphyromonas circumdentaria0.32%Prevotella denticola1.52%Prevotella oris0.54%Prevotella intermedia0.45%Prevotella nigrescens0.61%Prevotella ruminicola2.41%Capnocytophaga gingivalis0.44%Streptococcus downei7.77%Streptococcus salivarius4.61%Branch length scale: 1.0631 substitutions/site across the 500-unit horizontal axis. Root arbitrary.

Public raw read files

Files contain the original full run. Downloads are hosted by ENA.

FileType / bytesMD5
SRR12522741_1.fastq.gzFASTQ / 67936513377f63f33a331fcd2909da32e2286c7cf

Analysis provenance

marker16S
pipelineArchaions v18.8
read_samplingFirst available reads, not random subsampling
input_sha256e5c20118136b61b2e2092263a818f1d799c2020aae7d6e691d1a47eae3c0aeb1
median_read_length1427.0
reads_in_length_window9360
length_window_bp[1200, 2000]
quality_scoresUnavailable in fetched FASTA; no measured quality filtering
classifier_stats{"marker": "16s", "pipeline": "v18.8", "gpu": "RTX-PRO-6000", "input_path": "volume-fastq-gz", "reads_in": 10000, "reads_skipped": 2301, "elapsed_seconds": 1.0249937530000004, "reads_submitted": 10000, "reads_after_filter": 10000, "adapter_scan": {"sampled": 200, "matched": 1, "fraction": 0.005, "adapters_found": {"BC_FLANK": 1}, "should_trim": false}, "porechop_ran": false, "porechop_reason": "fast-path: no adapter trimming required"}

Full public metadata

All retrieved values are included. Blank source values are displayed as “Not reported”. Expand the sections below to inspect the full record.

ENA run fields (195)
run_accessionSRR12522741
ageNot reported
alignedNot reported
altitudeNot reported
assembly_qualityNot reported
assembly_softwareNot reported
bam_asperaNot reported
bam_bytesNot reported
bam_file_roleNot reported
bam_ftpNot reported
bam_galaxyNot reported
bam_md5Not reported
base_count650743183
binning_softwareNot reported
bio_materialNot reported
bisulfite_protocolNot reported
broad_scale_environmental_contextNot reported
broker_nameNot reported
cage_protocolNot reported
cell_lineNot reported
cell_typeNot reported
center_nameSUB7951364
checklistNot reported
chip_ab_providerNot reported
chip_protocolNot reported
chip_targetNot reported
collected_byNot reported
collection_datenot applicable
collection_date_endNot reported
collection_date_startNot reported
completeness_scoreNot reported
contamination_scoreNot reported
control_experimentNot reported
countryUnited Arab Emirates
cultivarNot reported
culture_collectionNot reported
datahubdcc_metagenome
depthNot reported
descriptionMinION sequencing: metagenomic
dev_stageNot reported
diseaseNot reported
dnase_protocolNot reported
ecotypeNot reported
elevationNot reported
environment_biomeNot reported
environment_featureNot reported
environment_materialNot reported
environmental_mediumNot reported
environmental_sampleNot reported
experiment_accessionSRX9012873
experiment_aliasX15-P
experiment_targetNot reported
experiment_titleMinION sequencing: metagenomic
experimental_factorNot reported
experimental_protocolNot reported
extraction_protocolNot reported
faang_library_selectionNot reported
fastq_asperafasp.sra.ebi.ac.uk:/vol1/fastq/SRR125/041/SRR12522741/SRR12522741_1.fastq.gz
fastq_bytes679365133
fastq_file_roleGENERATED_FILE
fastq_ftpftp.sra.ebi.ac.uk/vol1/fastq/SRR125/041/SRR12522741/SRR12522741_1.fastq.gz
fastq_galaxyftp.sra.ebi.ac.uk/vol1/fastq/SRR125/041/SRR12522741/SRR12522741_1.fastq.gz
fastq_md577f63f33a331fcd2909da32e2286c7cf
file_locationNot reported
first_created2021-10-01
first_public2021-10-01
germlineNot reported
hi_c_protocolNot reported
hostHomo sapiens
host_body_siteNot reported
host_genotypeNot reported
host_gravidityNot reported
host_growth_conditionsNot reported
host_phenotypeNot reported
host_scientific_nameHomo sapiens
host_sexNot reported
host_statusNot reported
host_tax_id9606
identified_byNot reported
instrument_modelMinION
instrument_platformOXFORD_NANOPORE
investigation_typeNot reported
isolatemixed1
isolation_sourceoral
last_updated2021-10-01
latNot reported
library_construction_protocolNot reported
library_gen_protocolNot reported
library_layoutSINGLE
library_max_fragment_sizeNot reported
library_min_fragment_sizeNot reported
library_nameX15-P
library_pcr_isolation_protocolNot reported
library_prep_dateNot reported
library_prep_date_formatNot reported
library_prep_latitudeNot reported
library_prep_locationNot reported
library_prep_longitudeNot reported
library_selectionPCR
library_sourceGENOMIC
library_strategyAMPLICON
local_environmental_contextNot reported
locationNot reported
location_endNot reported
location_startNot reported
lonNot reported
marine_regionNot reported
mating_typeNot reported
ncbi_reporting_standardMetagenome or environmental
nominal_lengthNot reported
nominal_sdevNot reported
pcr_isolation_protocolNot reported
phNot reported
project_nameNot reported
protocol_labelNot reported
read_count466205
read_strandNot reported
restriction_enzymeNot reported
restriction_enzyme_target_sequenceNot reported
restriction_siteNot reported
rna_integrity_numNot reported
rna_prep_3_protocolNot reported
rna_prep_5_protocolNot reported
rna_purity_230_ratioNot reported
rna_purity_280_ratioNot reported
rt_prep_protocolNot reported
run_aliasX15.fastq
run_dateNot reported
salinityNot reported
sample_accessionSAMN15887596
sample_aliasX15
sample_capture_statusNot reported
sample_collectionNot reported
sample_descriptionMetagenome or environmental sample from human oral metagenome
sample_materialNot reported
sample_prep_intervalNot reported
sample_prep_interval_unitsNot reported
sample_storageNot reported
sample_storage_processingNot reported
sample_titleMetagenome or environmental sample from human oral metagenome
sampling_campaignNot reported
sampling_platformNot reported
sampling_siteNot reported
scientific_namehuman oral metagenome
secondary_projectNot reported
secondary_sample_accessionSRS7263885
secondary_study_accessionSRP278853
sequencing_dateNot reported
sequencing_date_formatNot reported
sequencing_locationNot reported
sequencing_longitudeNot reported
sequencing_methodNot reported
sequencing_primer_catalogNot reported
sequencing_primer_lotNot reported
sequencing_primer_providerNot reported
serotypeNot reported
serovarNot reported
sexNot reported
specimen_voucherNot reported
sra_asperaNot reported
sra_bytesNot reported
sra_file_roleNot reported
sra_ftpNot reported
sra_galaxyNot reported
sra_md5Not reported
statuspublic
strainmixed
study_accessionPRJNA658726
study_aliasPRJNA658726
study_titleThe impact of smoking different tobacco types on the subgingival microbiome and periodontal health: a pilot study
sub_speciesNot reported
sub_strainNot reported
submission_accessionSRA1117712
submission_toolNot reported
submitted_asperaNot reported
submitted_bytesNot reported
submitted_file_roleNot reported
submitted_formatNot reported
submitted_ftpNot reported
submitted_galaxyNot reported
submitted_host_sexNot reported
submitted_md5Not reported
submitted_read_typeNot reported
surveillance_targetNot reported
tagNot reported
target_geneNot reported
tax_id447426
tax_lineage1;2787823;12908;408169;410656;447426
taxonomic_classificationNot reported
taxonomic_identity_markerNot reported
temperatureNot reported
tissue_libNot reported
tissue_typeNot reported
transposase_protocolNot reported
varietyNot reported
BioSample record and attributes
aliasX15
titleMetagenome or environmental sample from human oral metagenome
descriptionNot reported
strainmixed
collection_datenot applicable
INSDC secondary accessionSRS7263885
aliquotn3
organismhuman oral metagenome
sample_typeSubgingival
isolatemixed1
NCBI submission packageMetagenome.environmental.1.0
periodontitisno-mild
smokingNon-smoker
hostHomo sapiens
geo_loc_nameUnited Arab Emirates
lat_lonmissing
isolation_sourceoral
BioSampleModelMetagenome or environmental
ENA-FIRST-PUBLIC2021-09-30
ENA-LAST-UPDATE2021-09-30
Curated cohort annotations and QC
runSRR12522741
sampleSAMN15887596
studyPRJNA658726
titleMetagenome or environmental sample from human oral metagenome
aliasX15
siteSubgingival
subjectNot reported
phenotypeNot reported
sexNot reported
smokingNon-smoker
periodontitisno-mild
time_pointNot reported
negative_controlFalse
lab_modelFalse
statecomplete
reads_input10000
species_reported55
human_primaryTrue

Model-based taxonomy; research use. This report does not provide a diagnosis or a validated health score.