archAIons
archAIons · MetaBiome Project · Oral · Public research data

SRR12522740

Metagenome or environmental sample from human oral metagenome

BioSample: SAMN15887597 · Study: PRJNA658726 · Body site: Subgingival

Interactive report · NCBI SRA · ENA

73Reported species
17Reported genera

Taxonomic abundance

Relative abundance is normalized among reported taxa. No reported taxa does not establish absence of organisms.

SpeciesGenusPhylumAbundance
Streptococcus mitisStreptococcusBacillota15.44888%
Haemophilus parainfluenzaeHaemophilusPseudomonadota12.02809%
Neisseria flavescensNeisseriaPseudomonadota8.39876%
Haemophilus haemolyticusHaemophilusPseudomonadota7.64894%
Streptococcus downeiStreptococcusBacillota6.19288%
Veillonella parvulaVeillonellaBacillota4.41603%
Streptococcus salivariusStreptococcusBacillota3.65750%
Gemella palaticanisGemellaBacillota3.18957%
Abiotrophia defectivaAbiotrophiaBacillota2.45512%
Streptococcus pneumoniaeStreptococcusBacillota2.30217%
Neisseria macacaeNeisseriaPseudomonadota2.25068%
Fusobacterium nucleatumFusobacteriumFusobacteriota2.00737%
Aggregatibacter aphrophilusAggregatibacterPseudomonadota1.75924%
Granulicatella adiacensGranulicatellaBacillota1.67203%
Haemophilus aegyptiusHaemophilusPseudomonadota1.52326%
Aggregatibacter segnisAggregatibacterPseudomonadota1.52063%
Gemella haemolysansGemellaBacillota1.42482%
Neisseria mucosaNeisseriaPseudomonadota1.42107%
Streptococcus gordoniiStreptococcusBacillota1.32852%
Haemophilus parahaemolyticusHaemophilusPseudomonadota1.31643%
Neisseria gonorrhoeaeNeisseriaPseudomonadota1.20839%
Fusobacterium perfoetensFusobacteriumFusobacteriota1.11407%
Gemella morbillorumGemellaBacillota0.98629%
Neisseria meningitidisNeisseriaPseudomonadota0.74862%
Neisseria elongataNeisseriaPseudomonadota0.65695%
Porphyromonas catoniaePorphyromonasBacteroidota0.63107%
Selenomonas flueggeiSelenomonasBacillota0.58549%
Haemophilus paraphrohaemolyticusHaemophilusPseudomonadota0.57488%
Streptococcus oralisStreptococcusBacillota0.55088%
Streptococcus infantisStreptococcusBacillota0.55064%
Streptococcus sanguinisStreptococcusBacillota0.54557%
Leptotrichia hongkongensisLeptotrichiaFusobacteriota0.52711%
Veillonella infantiumVeillonellaBacillota0.52300%
Streptococcus pyogenesStreptococcusBacillota0.51020%
Capnocytophaga canisCapnocytophagaBacteroidota0.44399%
Neisseria dentiaeNeisseriaPseudomonadota0.41445%
Fusobacterium russiiFusobacteriumFusobacteriota0.40037%
Haemophilus influenzaeHaemophilusPseudomonadota0.36195%
Porphyromonas pasteriPorphyromonasBacteroidota0.32924%
Veillonella disparVeillonellaBacillota0.32840%
Lachnoanaerobaculum saburreumLachnoanaerobaculumBacillota0.32787%
Streptococcus cristatusStreptococcusBacillota0.30233%
Neisseria subflavaNeisseriaPseudomonadota0.26193%
Neisseria shayeganiiNeisseriaPseudomonadota0.25212%
Campylobacter gracilisCampylobacterPseudomonadota0.25168%
Campylobacter hyointestinalisCampylobacterPseudomonadota0.24890%
Granulicatella elegansGranulicatellaBacillota0.24772%
Streptococcus alactolyticusStreptococcusBacillota0.22038%
Neisseria canisNeisseriaPseudomonadota0.21939%
Streptococcus parasanguinisStreptococcusBacillota0.20916%
Lachnoanaerobaculum oraleLachnoanaerobaculumBacillota0.20558%
Granulicatella balaenopteraeGranulicatellaBacillota0.19677%
Gemella cuniculiGemellaBacillota0.19572%
Prevotella ruminicolaPrevotellaBacteroidota0.19028%
Campylobacter sputorumCampylobacterPseudomonadota0.19001%
Streptococcus marmotaeStreptococcusBacillota0.18514%
Streptococcus anginosusStreptococcusBacillota0.18041%
Streptococcus gallolyticusStreptococcusBacillota0.17997%
Campylobacter showaeCampylobacterPseudomonadota0.17890%
Veillonella atypicaVeillonellaBacillota0.16289%
Haemophilus sputorumHaemophilusPseudomonadota0.15476%
Streptococcus sobrinusStreptococcusBacillota0.14623%
Dialister invisusDialisterBacillota0.14148%
Lachnoanaerobaculum umeaenseLachnoanaerobaculumBacillota0.14032%
Porphyromonas macacaePorphyromonasBacteroidota0.13259%
Streptococcus suisStreptococcusBacillota0.13148%
Streptococcus australisStreptococcusBacillota0.12856%
Streptococcus periodonticumStreptococcusBacillota0.11660%
Fusobacterium simiaeFusobacteriumFusobacteriota0.11611%
Streptococcus intermediusStreptococcusBacillota0.11341%
Streptococcus orisrattiStreptococcusBacillota0.11279%
Porphyromonas gingivalisPorphyromonasBacteroidota0.10734%
Streptococcus macacaeStreptococcusBacillota0.09766%

Reference-based 16S phylogeny

72 of 73 reported species have an eligible reference. MAFFT alignment and FastTree GTR+CAT tree, pruned to this sample. This is not a phylogeny inferred from sample reads or ASVs. Root placement is arbitrary; branch lengths are substitutions per site.

Streptococcus periodonticum0.12%Streptococcus anginosus0.18%Streptococcus intermedius0.11%Streptococcus cristatus0.30%Streptococcus parasanguinis0.21%Streptococcus infantis0.55%Streptococcus oralis0.55%Streptococcus mitis15.45%Streptococcus pneumoniae2.30%Streptococcus australis0.13%Streptococcus marmotae0.19%Streptococcus gordonii1.33%Streptococcus sanguinis0.55%Streptococcus suis0.13%Streptococcus alactolyticus0.22%Streptococcus gallolyticus0.18%Streptococcus orisratti0.11%Streptococcus pyogenes0.51%Abiotrophia defectiva2.46%Granulicatella balaenopterae0.20%Granulicatella adiacens1.67%Granulicatella elegans0.25%Gemella palaticanis3.19%Gemella cuniculi0.20%Gemella haemolysans1.42%Gemella morbillorum0.99%Veillonella dispar0.33%Veillonella infantium0.52%Veillonella parvula4.42%Veillonella atypica0.16%Dialister invisus0.14%Selenomonas flueggei0.59%Lachnoanaerobaculum umeaense0.14%Lachnoanaerobaculum orale0.21%Lachnoanaerobaculum saburreum0.33%Fusobacterium perfoetens1.11%Fusobacterium russii0.40%Fusobacterium nucleatum2.01%Fusobacterium simiae0.12%Leptotrichia hongkongensis0.53%Campylobacter sputorum0.19%Campylobacter gracilis0.25%Campylobacter showae0.18%Campylobacter hyointestinalis0.25%Neisseria canis0.22%Neisseria dentiae0.41%Neisseria shayeganii0.25%Neisseria subflava0.26%Neisseria mucosa1.42%Neisseria macacae2.25%Neisseria meningitidis0.75%Neisseria gonorrhoeae1.21%Neisseria flavescens8.40%Neisseria elongata0.66%Haemophilus parainfluenzae12.03%Haemophilus sputorum0.15%Haemophilus parahaemolyticus1.32%Haemophilus paraphrohaemolyticus0.57%Haemophilus haemolyticus7.65%Haemophilus aegyptius1.52%Haemophilus influenzae0.36%Aggregatibacter segnis1.52%Aggregatibacter aphrophilus1.76%Porphyromonas gingivalis0.11%Porphyromonas catoniae0.63%Porphyromonas pasteri0.33%Porphyromonas macacae0.13%Prevotella ruminicola0.19%Streptococcus macacae0.10%Streptococcus sobrinus0.15%Streptococcus downei6.19%Streptococcus salivarius3.66%Branch length scale: 1.0175 substitutions/site across the 500-unit horizontal axis. Root arbitrary.

Public raw read files

Files contain the original full run. Downloads are hosted by ENA.

FileType / bytesMD5
SRR12522740_1.fastq.gzFASTQ / 924823703ce4a145088f694d51091524c4544c70b

Analysis provenance

marker16S
pipelineArchaions v18.8
read_samplingFirst available reads, not random subsampling
input_sha256a4a3585b47363fea526c7d7f9514dc2692c75350d9c1b0c78d2b54c9a8f9ac01
median_read_length1432.0
reads_in_length_window28292
length_window_bp[1200, 2000]
quality_scoresUnavailable in fetched FASTA; no measured quality filtering
classifier_stats{"marker": "16s", "pipeline": "v18.8", "gpu": "RTX-PRO-6000", "input_path": "volume-fastq-gz", "reads_in": 30000, "reads_skipped": 9323, "elapsed_seconds": 2.5301884419999965, "reads_submitted": 30000, "reads_after_filter": 30000, "adapter_scan": {"sampled": 200, "matched": 20, "fraction": 0.1, "adapters_found": {"BC_FLANK": 19, "27F": 1}, "should_trim": true}, "porechop_ran": false, "porechop_reason": "fast-path: no adapter trimming required"}

Full public metadata

All retrieved values are included. Blank source values are displayed as “Not reported”. Expand the sections below to inspect the full record.

ENA run fields (195)
run_accessionSRR12522740
ageNot reported
alignedNot reported
altitudeNot reported
assembly_qualityNot reported
assembly_softwareNot reported
bam_asperaNot reported
bam_bytesNot reported
bam_file_roleNot reported
bam_ftpNot reported
bam_galaxyNot reported
bam_md5Not reported
base_count897757805
binning_softwareNot reported
bio_materialNot reported
bisulfite_protocolNot reported
broad_scale_environmental_contextNot reported
broker_nameNot reported
cage_protocolNot reported
cell_lineNot reported
cell_typeNot reported
center_nameSUB7951364
checklistNot reported
chip_ab_providerNot reported
chip_protocolNot reported
chip_targetNot reported
collected_byNot reported
collection_datenot applicable
collection_date_endNot reported
collection_date_startNot reported
completeness_scoreNot reported
contamination_scoreNot reported
control_experimentNot reported
countryUnited Arab Emirates
cultivarNot reported
culture_collectionNot reported
datahubdcc_metagenome
depthNot reported
descriptionMinION sequencing: metagenomic
dev_stageNot reported
diseaseNot reported
dnase_protocolNot reported
ecotypeNot reported
elevationNot reported
environment_biomeNot reported
environment_featureNot reported
environment_materialNot reported
environmental_mediumNot reported
environmental_sampleNot reported
experiment_accessionSRX9012874
experiment_aliasX16-P
experiment_targetNot reported
experiment_titleMinION sequencing: metagenomic
experimental_factorNot reported
experimental_protocolNot reported
extraction_protocolNot reported
faang_library_selectionNot reported
fastq_asperafasp.sra.ebi.ac.uk:/vol1/fastq/SRR125/040/SRR12522740/SRR12522740_1.fastq.gz
fastq_bytes924823703
fastq_file_roleGENERATED_FILE
fastq_ftpftp.sra.ebi.ac.uk/vol1/fastq/SRR125/040/SRR12522740/SRR12522740_1.fastq.gz
fastq_galaxyftp.sra.ebi.ac.uk/vol1/fastq/SRR125/040/SRR12522740/SRR12522740_1.fastq.gz
fastq_md5ce4a145088f694d51091524c4544c70b
file_locationNot reported
first_created2021-10-01
first_public2021-10-01
germlineNot reported
hi_c_protocolNot reported
hostHomo sapiens
host_body_siteNot reported
host_genotypeNot reported
host_gravidityNot reported
host_growth_conditionsNot reported
host_phenotypeNot reported
host_scientific_nameHomo sapiens
host_sexNot reported
host_statusNot reported
host_tax_id9606
identified_byNot reported
instrument_modelMinION
instrument_platformOXFORD_NANOPORE
investigation_typeNot reported
isolatemixed1
isolation_sourceoral
last_updated2021-10-01
latNot reported
library_construction_protocolNot reported
library_gen_protocolNot reported
library_layoutSINGLE
library_max_fragment_sizeNot reported
library_min_fragment_sizeNot reported
library_nameX16-P
library_pcr_isolation_protocolNot reported
library_prep_dateNot reported
library_prep_date_formatNot reported
library_prep_latitudeNot reported
library_prep_locationNot reported
library_prep_longitudeNot reported
library_selectionPCR
library_sourceGENOMIC
library_strategyAMPLICON
local_environmental_contextNot reported
locationNot reported
location_endNot reported
location_startNot reported
lonNot reported
marine_regionNot reported
mating_typeNot reported
ncbi_reporting_standardMetagenome or environmental
nominal_lengthNot reported
nominal_sdevNot reported
pcr_isolation_protocolNot reported
phNot reported
project_nameNot reported
protocol_labelNot reported
read_count642059
read_strandNot reported
restriction_enzymeNot reported
restriction_enzyme_target_sequenceNot reported
restriction_siteNot reported
rna_integrity_numNot reported
rna_prep_3_protocolNot reported
rna_prep_5_protocolNot reported
rna_purity_230_ratioNot reported
rna_purity_280_ratioNot reported
rt_prep_protocolNot reported
run_aliasX16.fastq
run_dateNot reported
salinityNot reported
sample_accessionSAMN15887597
sample_aliasX16
sample_capture_statusNot reported
sample_collectionNot reported
sample_descriptionMetagenome or environmental sample from human oral metagenome
sample_materialNot reported
sample_prep_intervalNot reported
sample_prep_interval_unitsNot reported
sample_storageNot reported
sample_storage_processingNot reported
sample_titleMetagenome or environmental sample from human oral metagenome
sampling_campaignNot reported
sampling_platformNot reported
sampling_siteNot reported
scientific_namehuman oral metagenome
secondary_projectNot reported
secondary_sample_accessionSRS7263886
secondary_study_accessionSRP278853
sequencing_dateNot reported
sequencing_date_formatNot reported
sequencing_locationNot reported
sequencing_longitudeNot reported
sequencing_methodNot reported
sequencing_primer_catalogNot reported
sequencing_primer_lotNot reported
sequencing_primer_providerNot reported
serotypeNot reported
serovarNot reported
sexNot reported
specimen_voucherNot reported
sra_asperaNot reported
sra_bytesNot reported
sra_file_roleNot reported
sra_ftpNot reported
sra_galaxyNot reported
sra_md5Not reported
statuspublic
strainmixed
study_accessionPRJNA658726
study_aliasPRJNA658726
study_titleThe impact of smoking different tobacco types on the subgingival microbiome and periodontal health: a pilot study
sub_speciesNot reported
sub_strainNot reported
submission_accessionSRA1117712
submission_toolNot reported
submitted_asperaNot reported
submitted_bytesNot reported
submitted_file_roleNot reported
submitted_formatNot reported
submitted_ftpNot reported
submitted_galaxyNot reported
submitted_host_sexNot reported
submitted_md5Not reported
submitted_read_typeNot reported
surveillance_targetNot reported
tagNot reported
target_geneNot reported
tax_id447426
tax_lineage1;2787823;12908;408169;410656;447426
taxonomic_classificationNot reported
taxonomic_identity_markerNot reported
temperatureNot reported
tissue_libNot reported
tissue_typeNot reported
transposase_protocolNot reported
varietyNot reported
BioSample record and attributes
aliasX16
titleMetagenome or environmental sample from human oral metagenome
descriptionNot reported
strainmixed
collection_datenot applicable
INSDC secondary accessionSRS7263886
aliquotn4
organismhuman oral metagenome
sample_typeSubgingival
isolatemixed1
NCBI submission packageMetagenome.environmental.1.0
periodontitisno-mild
smokingNon-smoker
hostHomo sapiens
geo_loc_nameUnited Arab Emirates
lat_lonmissing
isolation_sourceoral
BioSampleModelMetagenome or environmental
ENA-FIRST-PUBLIC2021-09-30
ENA-LAST-UPDATE2021-09-30
Curated cohort annotations and QC
runSRR12522740
sampleSAMN15887597
studyPRJNA658726
titleMetagenome or environmental sample from human oral metagenome
aliasX16
siteSubgingival
subjectNot reported
phenotypeNot reported
sexNot reported
smokingNon-smoker
periodontitisno-mild
time_pointNot reported
negative_controlFalse
lab_modelFalse
statecomplete
reads_input30000
species_reported73
human_primaryTrue

Model-based taxonomy; research use. This report does not provide a diagnosis or a validated health score.