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archAIons · MetaBiome Project · Oral · Public research data

SRR12522736

Metagenome or environmental sample from human oral metagenome

BioSample: SAMN15887601 · Study: PRJNA658726 · Body site: Subgingival

Interactive report · NCBI SRA · ENA

90Reported species
22Reported genera

Taxonomic abundance

Relative abundance is normalized among reported taxa. No reported taxa does not establish absence of organisms.

SpeciesGenusPhylumAbundance
Streptococcus mitisStreptococcusBacillota13.65971%
Veillonella parvulaVeillonellaBacillota11.31569%
Fusobacterium nucleatumFusobacteriumFusobacteriota7.01265%
Streptococcus downeiStreptococcusBacillota6.70497%
Selenomonas flueggeiSelenomonasBacillota5.75079%
Fusobacterium perfoetensFusobacteriumFusobacteriota4.50292%
Streptococcus salivariusStreptococcusBacillota3.17727%
Gemella palaticanisGemellaBacillota2.66011%
Eubacterium ramulusEubacteriumBacillota2.32040%
Filifactor alocisFilifactorBacillota2.00743%
Streptococcus pneumoniaeStreptococcusBacillota1.99067%
Dialister invisusDialisterBacillota1.84513%
Fusobacterium russiiFusobacteriumFusobacteriota1.63664%
Porphyromonas gingivalisPorphyromonasBacteroidota1.47020%
Peptostreptococcus anaerobiusPeptostreptococcusBacillota1.19799%
Selenomonas dianaeSelenomonasBacillota1.10237%
Streptococcus anginosusStreptococcusBacillota1.05660%
Selenomonas sputigenaSelenomonasBacillota1.05507%
Veillonella infantiumVeillonellaBacillota1.05303%
Gemella haemolysansGemellaBacillota1.04929%
Filifactor villosusFilifactorBacillota1.01006%
Neisseria flavescensNeisseriaPseudomonadota0.88834%
Prevotella ruminicolaPrevotellaBacteroidota0.87747%
Parvimonas micraParvimonasBacillota0.86927%
Megasphaera elsdeniiMegasphaeraBacillota0.86722%
Campylobacter hyointestinalisCampylobacterPseudomonadota0.85756%
Capnocytophaga canisCapnocytophagaBacteroidota0.81142%
Granulicatella adiacensGranulicatellaBacillota0.73473%
Campylobacter showaeCampylobacterPseudomonadota0.73319%
Gemella morbillorumGemellaBacillota0.72483%
Streptococcus oralisStreptococcusBacillota0.71566%
Megasphaera stantoniiMegasphaeraBacillota0.70412%
Eubacterium limosumEubacteriumBacillota0.69135%
Streptococcus infantisStreptococcusBacillota0.66095%
Campylobacter gracilisCampylobacterPseudomonadota0.62064%
Veillonella disparVeillonellaBacillota0.59195%
Campylobacter sputorumCampylobacterPseudomonadota0.58304%
Dialister hominisDialisterBacillota0.55829%
Megasphaera coliMegasphaeraBacillota0.49594%
Streptococcus pyogenesStreptococcusBacillota0.47200%
Streptococcus sanguinisStreptococcusBacillota0.45739%
Fusobacterium simiaeFusobacteriumFusobacteriota0.45106%
Dialister pneumosintesDialisterBacillota0.44512%
Prevotella nigrescensPrevotellaBacteroidota0.43937%
Streptococcus cristatusStreptococcusBacillota0.42590%
Aggregatibacter segnisAggregatibacterPseudomonadota0.42385%
Selenomonas infelixSelenomonasBacillota0.41044%
Streptococcus gordoniiStreptococcusBacillota0.40313%
Neisseria macacaeNeisseriaPseudomonadota0.38214%
Ottowia flavaOttowiaPseudomonadota0.36789%
Veillonella rodentiumVeillonellaBacillota0.34167%
Pseudoramibacter alactolyticusPseudoramibacterBacillota0.32631%
Treponema pectinovorumTreponemaSpirochaetota0.32301%
Fusobacterium periodonticumFusobacteriumFusobacteriota0.32290%
Selenomonas ruminantiumSelenomonasBacillota0.31981%
Prevotella intermediaPrevotellaBacteroidota0.31006%
Campylobacter concisusCampylobacterPseudomonadota0.30660%
Peptostreptococcus stomatisPeptostreptococcusBacillota0.28801%
Veillonella atypicaVeillonellaBacillota0.27938%
Streptococcus alactolyticusStreptococcusBacillota0.27700%
Aggregatibacter actinomycetemcomitansAggregatibacterPseudomonadota0.27348%
Tannerella forsythiaTannerellaBacteroidota0.27054%
Selenomonas artemidisSelenomonasBacillota0.26899%
Selenomonas noxiaSelenomonasBacillota0.26825%
Ottowia beijingensisOttowiaPseudomonadota0.26257%
Megasphaera cerevisiaeMegasphaeraBacillota0.25465%
Prevotella melaninogenicaPrevotellaBacteroidota0.24943%
Campylobacter curvusCampylobacterPseudomonadota0.22587%
Gemella cuniculiGemellaBacillota0.21784%
Megasphaera hexanoicaMegasphaeraBacillota0.21646%
Treponema denticolaTreponemaSpirochaetota0.19583%
Prevotella orisPrevotellaBacteroidota0.19429%
Capnocytophaga granulosaCapnocytophagaBacteroidota0.19416%
Eubacterium barkeriEubacteriumBacillota0.18821%
Treponema succinifaciensTreponemaSpirochaetota0.18804%
Eubacterium xylanophilumEubacteriumBacillota0.18074%
Prevotella denticolaPrevotellaBacteroidota0.17422%
Streptococcus suisStreptococcusBacillota0.16991%
Streptococcus orisrattiStreptococcusBacillota0.16224%
Streptococcus intermediusStreptococcusBacillota0.15225%
Porphyromonas macacaePorphyromonasBacteroidota0.15064%
Granulicatella elegansGranulicatellaBacillota0.14887%
Streptococcus australisStreptococcusBacillota0.14639%
Streptococcus marmotaeStreptococcusBacillota0.14208%
Dialister propionicifaciensDialisterBacillota0.14034%
Eubacterium multiformeEubacteriumBacillota0.13946%
Eubacterium callanderiEubacteriumBacillota0.13129%
Megasphaera sueciensisMegasphaeraBacillota0.12962%
Streptococcus periodonticumStreptococcusBacillota0.12285%
Capnocytophaga gingivalisCapnocytophagaBacteroidota0.10250%

Reference-based 16S phylogeny

89 of 90 reported species have an eligible reference. MAFFT alignment and FastTree GTR+CAT tree, pruned to this sample. This is not a phylogeny inferred from sample reads or ASVs. Root placement is arbitrary; branch lengths are substitutions per site.

Streptococcus periodonticum0.12%Streptococcus anginosus1.06%Streptococcus intermedius0.15%Streptococcus cristatus0.43%Streptococcus infantis0.66%Streptococcus oralis0.72%Streptococcus mitis13.66%Streptococcus pneumoniae1.99%Streptococcus australis0.15%Streptococcus marmotae0.14%Streptococcus gordonii0.40%Streptococcus sanguinis0.46%Streptococcus suis0.17%Streptococcus alactolyticus0.28%Streptococcus orisratti0.16%Streptococcus pyogenes0.47%Granulicatella adiacens0.73%Granulicatella elegans0.15%Gemella palaticanis2.66%Gemella cuniculi0.22%Gemella haemolysans1.05%Gemella morbillorum0.72%Veillonella dispar0.59%Veillonella infantium1.05%Veillonella parvula11.32%Veillonella atypica0.28%Veillonella rodentium0.34%Megasphaera stantonii0.70%Megasphaera cerevisiae0.25%Megasphaera coli0.50%Megasphaera sueciensis0.13%Megasphaera elsdenii0.87%Megasphaera hexanoica0.22%Dialister invisus1.85%Dialister pneumosintes0.45%Dialister propionicifaciens0.14%Dialister hominis0.56%Selenomonas ruminantium0.32%Selenomonas sputigena1.06%Selenomonas artemidis0.27%Selenomonas noxia0.27%Selenomonas flueggei5.75%Selenomonas dianae1.10%Selenomonas infelix0.41%Eubacterium xylanophilum0.18%Eubacterium ramulus2.32%Eubacterium multiforme0.14%Pseudoramibacter alactolyticus0.33%Eubacterium barkeri0.19%Eubacterium callanderi0.13%Eubacterium limosum0.69%Parvimonas micra0.87%Peptostreptococcus anaerobius1.20%Peptostreptococcus stomatis0.29%Filifactor alocis2.01%Filifactor villosus1.01%Fusobacterium perfoetens4.50%Fusobacterium russii1.64%Fusobacterium periodonticum0.32%Fusobacterium nucleatum7.01%Fusobacterium simiae0.45%Campylobacter sputorum0.58%Campylobacter gracilis0.62%Campylobacter showae0.73%Campylobacter curvus0.23%Campylobacter concisus0.31%Campylobacter hyointestinalis0.86%Neisseria macacae0.38%Neisseria flavescens0.89%Ottowia flava0.37%Ottowia beijingensis0.26%Aggregatibacter segnis0.42%Aggregatibacter actinomycetemcomitans0.27%Porphyromonas gingivalis1.47%Porphyromonas macacae0.15%Tannerella forsythia0.27%Prevotella melaninogenica0.25%Prevotella denticola0.17%Prevotella oris0.19%Prevotella intermedia0.31%Prevotella nigrescens0.44%Prevotella ruminicola0.88%Capnocytophaga gingivalis0.10%Capnocytophaga granulosa0.19%Treponema denticola0.20%Treponema pectinovorum0.32%Treponema succinifaciens0.19%Streptococcus downei6.70%Streptococcus salivarius3.18%Branch length scale: 1.0631 substitutions/site across the 500-unit horizontal axis. Root arbitrary.

Public raw read files

Files contain the original full run. Downloads are hosted by ENA.

FileType / bytesMD5
SRR12522736_1.fastq.gzFASTQ / 946495821c4677b20b5e7826967c6ab8165c5bb94

Analysis provenance

marker16S
pipelineArchaions v18.8
read_samplingFirst available reads, not random subsampling
input_sha25651a31e03f5655b411027569eeb74b30c85be6cc4650f724061716663c16bdac0
median_read_length1427.0
reads_in_length_window28567
length_window_bp[1200, 2000]
quality_scoresUnavailable in fetched FASTA; no measured quality filtering
classifier_stats{"marker": "16s", "pipeline": "v18.8", "gpu": "RTX-PRO-6000", "input_path": "volume-fastq-gz", "reads_in": 30000, "reads_skipped": 8812, "elapsed_seconds": 2.560223752000013, "reads_submitted": 30000, "reads_after_filter": 30000, "adapter_scan": {"sampled": 200, "matched": 2, "fraction": 0.01, "adapters_found": {"BC_FLANK": 2}, "should_trim": false}, "porechop_ran": false, "porechop_reason": "fast-path: no adapter trimming required"}

Full public metadata

All retrieved values are included. Blank source values are displayed as “Not reported”. Expand the sections below to inspect the full record.

ENA run fields (195)
run_accessionSRR12522736
ageNot reported
alignedNot reported
altitudeNot reported
assembly_qualityNot reported
assembly_softwareNot reported
bam_asperaNot reported
bam_bytesNot reported
bam_file_roleNot reported
bam_ftpNot reported
bam_galaxyNot reported
bam_md5Not reported
base_count903094729
binning_softwareNot reported
bio_materialNot reported
bisulfite_protocolNot reported
broad_scale_environmental_contextNot reported
broker_nameNot reported
cage_protocolNot reported
cell_lineNot reported
cell_typeNot reported
center_nameSUB7951364
checklistNot reported
chip_ab_providerNot reported
chip_protocolNot reported
chip_targetNot reported
collected_byNot reported
collection_datenot applicable
collection_date_endNot reported
collection_date_startNot reported
completeness_scoreNot reported
contamination_scoreNot reported
control_experimentNot reported
countryUnited Arab Emirates
cultivarNot reported
culture_collectionNot reported
datahubdcc_metagenome
depthNot reported
descriptionMinION sequencing: metagenomic
dev_stageNot reported
diseaseNot reported
dnase_protocolNot reported
ecotypeNot reported
elevationNot reported
environment_biomeNot reported
environment_featureNot reported
environment_materialNot reported
environmental_mediumNot reported
environmental_sampleNot reported
experiment_accessionSRX9012878
experiment_aliasX87-P
experiment_targetNot reported
experiment_titleMinION sequencing: metagenomic
experimental_factorNot reported
experimental_protocolNot reported
extraction_protocolNot reported
faang_library_selectionNot reported
fastq_asperafasp.sra.ebi.ac.uk:/vol1/fastq/SRR125/036/SRR12522736/SRR12522736_1.fastq.gz
fastq_bytes946495821
fastq_file_roleGENERATED_FILE
fastq_ftpftp.sra.ebi.ac.uk/vol1/fastq/SRR125/036/SRR12522736/SRR12522736_1.fastq.gz
fastq_galaxyftp.sra.ebi.ac.uk/vol1/fastq/SRR125/036/SRR12522736/SRR12522736_1.fastq.gz
fastq_md5c4677b20b5e7826967c6ab8165c5bb94
file_locationNot reported
first_created2021-10-01
first_public2021-10-01
germlineNot reported
hi_c_protocolNot reported
hostHomo sapiens
host_body_siteNot reported
host_genotypeNot reported
host_gravidityNot reported
host_growth_conditionsNot reported
host_phenotypeNot reported
host_scientific_nameHomo sapiens
host_sexNot reported
host_statusNot reported
host_tax_id9606
identified_byNot reported
instrument_modelMinION
instrument_platformOXFORD_NANOPORE
investigation_typeNot reported
isolatemixed1
isolation_sourceoral
last_updated2021-10-01
latNot reported
library_construction_protocolNot reported
library_gen_protocolNot reported
library_layoutSINGLE
library_max_fragment_sizeNot reported
library_min_fragment_sizeNot reported
library_nameX87-P
library_pcr_isolation_protocolNot reported
library_prep_dateNot reported
library_prep_date_formatNot reported
library_prep_latitudeNot reported
library_prep_locationNot reported
library_prep_longitudeNot reported
library_selectionPCR
library_sourceGENOMIC
library_strategyAMPLICON
local_environmental_contextNot reported
locationNot reported
location_endNot reported
location_startNot reported
lonNot reported
marine_regionNot reported
mating_typeNot reported
ncbi_reporting_standardMetagenome or environmental
nominal_lengthNot reported
nominal_sdevNot reported
pcr_isolation_protocolNot reported
phNot reported
project_nameNot reported
protocol_labelNot reported
read_count649360
read_strandNot reported
restriction_enzymeNot reported
restriction_enzyme_target_sequenceNot reported
restriction_siteNot reported
rna_integrity_numNot reported
rna_prep_3_protocolNot reported
rna_prep_5_protocolNot reported
rna_purity_230_ratioNot reported
rna_purity_280_ratioNot reported
rt_prep_protocolNot reported
run_aliasX87.fastq
run_dateNot reported
salinityNot reported
sample_accessionSAMN15887601
sample_aliasX87
sample_capture_statusNot reported
sample_collectionNot reported
sample_descriptionMetagenome or environmental sample from human oral metagenome
sample_materialNot reported
sample_prep_intervalNot reported
sample_prep_interval_unitsNot reported
sample_storageNot reported
sample_storage_processingNot reported
sample_titleMetagenome or environmental sample from human oral metagenome
sampling_campaignNot reported
sampling_platformNot reported
sampling_siteNot reported
scientific_namehuman oral metagenome
secondary_projectNot reported
secondary_sample_accessionSRS7263890
secondary_study_accessionSRP278853
sequencing_dateNot reported
sequencing_date_formatNot reported
sequencing_locationNot reported
sequencing_longitudeNot reported
sequencing_methodNot reported
sequencing_primer_catalogNot reported
sequencing_primer_lotNot reported
sequencing_primer_providerNot reported
serotypeNot reported
serovarNot reported
sexNot reported
specimen_voucherNot reported
sra_asperaNot reported
sra_bytesNot reported
sra_file_roleNot reported
sra_ftpNot reported
sra_galaxyNot reported
sra_md5Not reported
statuspublic
strainmixed
study_accessionPRJNA658726
study_aliasPRJNA658726
study_titleThe impact of smoking different tobacco types on the subgingival microbiome and periodontal health: a pilot study
sub_speciesNot reported
sub_strainNot reported
submission_accessionSRA1117712
submission_toolNot reported
submitted_asperaNot reported
submitted_bytesNot reported
submitted_file_roleNot reported
submitted_formatNot reported
submitted_ftpNot reported
submitted_galaxyNot reported
submitted_host_sexNot reported
submitted_md5Not reported
submitted_read_typeNot reported
surveillance_targetNot reported
tagNot reported
target_geneNot reported
tax_id447426
tax_lineage1;2787823;12908;408169;410656;447426
taxonomic_classificationNot reported
taxonomic_identity_markerNot reported
temperatureNot reported
tissue_libNot reported
tissue_typeNot reported
transposase_protocolNot reported
varietyNot reported
BioSample record and attributes
aliasX87
titleMetagenome or environmental sample from human oral metagenome
descriptionNot reported
strainmixed
collection_datenot applicable
INSDC secondary accessionSRS7263890
aliquotn8
organismhuman oral metagenome
sample_typeSubgingival
isolatemixed1
NCBI submission packageMetagenome.environmental.1.0
periodontitisno-mild
smokingNon-smoker
hostHomo sapiens
geo_loc_nameUnited Arab Emirates
lat_lonmissing
isolation_sourceoral
BioSampleModelMetagenome or environmental
ENA-FIRST-PUBLIC2021-09-30
ENA-LAST-UPDATE2021-09-30
Curated cohort annotations and QC
runSRR12522736
sampleSAMN15887601
studyPRJNA658726
titleMetagenome or environmental sample from human oral metagenome
aliasX87
siteSubgingival
subjectNot reported
phenotypeNot reported
sexNot reported
smokingNon-smoker
periodontitisno-mild
time_pointNot reported
negative_controlFalse
lab_modelFalse
statecomplete
reads_input30000
species_reported90
human_primaryTrue

Model-based taxonomy; research use. This report does not provide a diagnosis or a validated health score.