archAIons
archAIons · MetaBiome Project · Oral · Public research data

SRR12522735

Metagenome or environmental sample from human oral metagenome

BioSample: SAMN15887602 · Study: PRJNA658726 · Body site: Subgingival

Interactive report · NCBI SRA · ENA

76Reported species
18Reported genera

Taxonomic abundance

Relative abundance is normalized among reported taxa. No reported taxa does not establish absence of organisms.

SpeciesGenusPhylumAbundance
Fusobacterium nucleatumFusobacteriumFusobacteriota9.85999%
Parvimonas micraParvimonasBacillota9.43466%
Streptococcus mitisStreptococcusBacillota8.99756%
Fusobacterium perfoetensFusobacteriumFusobacteriota5.49305%
Streptococcus downeiStreptococcusBacillota4.86190%
Eubacterium ramulusEubacteriumBacillota4.65565%
Gemella palaticanisGemellaBacillota4.40660%
Porphyromonas gingivalisPorphyromonasBacteroidota3.41633%
Mogibacterium timidumMogibacteriumBacillota3.38802%
Dialister invisusDialisterBacillota2.74090%
Selenomonas flueggeiSelenomonasBacillota2.64719%
Fusobacterium russiiFusobacteriumFusobacteriota2.53712%
Streptococcus salivariusStreptococcusBacillota2.41021%
Peptostreptococcus anaerobiusPeptostreptococcusBacillota2.27297%
Megasphaera elsdeniiMegasphaeraBacillota2.00153%
Megasphaera stantoniiMegasphaeraBacillota1.63506%
Gemella haemolysansGemellaBacillota1.45193%
Streptococcus pneumoniaeStreptococcusBacillota1.31020%
Pyramidobacter piscolensPyramidobacterSynergistota1.24332%
Streptococcus anginosusStreptococcusBacillota1.24014%
Tannerella forsythiaTannerellaBacteroidota1.18961%
Gemella morbillorumGemellaBacillota1.05165%
Granulicatella adiacensGranulicatellaBacillota0.95639%
Eubacterium limosumEubacteriumBacillota0.94537%
Mogibacterium pumilumMogibacteriumBacillota0.94369%
Campylobacter showaeCampylobacterPseudomonadota0.92513%
Campylobacter gracilisCampylobacterPseudomonadota0.82985%
Dialister hominisDialisterBacillota0.82601%
Campylobacter hyointestinalisCampylobacterPseudomonadota0.75701%
Neisseria flavescensNeisseriaPseudomonadota0.68643%
Campylobacter sputorumCampylobacterPseudomonadota0.68386%
Megasphaera hexanoicaMegasphaeraBacillota0.67415%
Ottowia flavaOttowiaPseudomonadota0.66792%
Fusobacterium simiaeFusobacteriumFusobacteriota0.63074%
Megasphaera coliMegasphaeraBacillota0.55945%
Streptococcus oralisStreptococcusBacillota0.52416%
Selenomonas dianaeSelenomonasBacillota0.51411%
Capnocytophaga canisCapnocytophagaBacteroidota0.50633%
Streptococcus infantisStreptococcusBacillota0.50408%
Selenomonas sputigenaSelenomonasBacillota0.46929%
Porphyromonas macacaePorphyromonasBacteroidota0.45971%
Streptococcus pyogenesStreptococcusBacillota0.44942%
Streptococcus cristatusStreptococcusBacillota0.42122%
Porphyromonas catoniaePorphyromonasBacteroidota0.41397%
Eubacterium pyruvativoransEubacteriumBacillota0.39824%
Peptostreptococcus stomatisPeptostreptococcusBacillota0.38852%
Fusobacterium periodonticumFusobacteriumFusobacteriota0.37671%
Mogibacterium diversumMogibacteriumBacillota0.37443%
Mogibacterium vescumMogibacteriumBacillota0.36756%
Streptococcus intermediusStreptococcusBacillota0.35054%
Gemella cuniculiGemellaBacillota0.33403%
Ottowia beijingensisOttowiaPseudomonadota0.32948%
Streptococcus gordoniiStreptococcusBacillota0.29845%
Megasphaera massiliensisMegasphaeraBacillota0.26883%
Eubacterium callanderiEubacteriumBacillota0.23908%
Porphyromonas gingivicanisPorphyromonasBacteroidota0.23629%
Neisseria macacaeNeisseriaPseudomonadota0.22496%
Megasphaera cerevisiaeMegasphaeraBacillota0.22198%
Capnocytophaga gingivalisCapnocytophagaBacteroidota0.21940%
Mogibacterium neglectumMogibacteriumBacillota0.21050%
Dialister propionicifaciensDialisterBacillota0.20648%
Selenomonas ruminantiumSelenomonasBacillota0.19356%
Ottowia thiooxydansOttowiaPseudomonadota0.18403%
Eubacterium barkeriEubacteriumBacillota0.17911%
Selenomonas noxiaSelenomonasBacillota0.17540%
Eubacterium xylanophilumEubacteriumBacillota0.17103%
Eubacterium multiformeEubacteriumBacillota0.16674%
Streptococcus gallolyticusStreptococcusBacillota0.15792%
Campylobacter rectusCampylobacterPseudomonadota0.15694%
Peptostreptococcus canisPeptostreptococcusBacillota0.15318%
Selenomonas infelixSelenomonasBacillota0.15216%
Streptococcus alactolyticusStreptococcusBacillota0.15072%
Megasphaera geminataMegasphaeraBacillota0.13479%
Dialister micraerophilusDialisterBacillota0.13385%
Megasphaera sueciensisMegasphaeraBacillota0.12920%
Megasphaera paucivoransMegasphaeraBacillota0.12199%

Reference-based 16S phylogeny

75 of 76 reported species have an eligible reference. MAFFT alignment and FastTree GTR+CAT tree, pruned to this sample. This is not a phylogeny inferred from sample reads or ASVs. Root placement is arbitrary; branch lengths are substitutions per site.

Streptococcus anginosus1.24%Streptococcus intermedius0.35%Streptococcus cristatus0.42%Streptococcus infantis0.50%Streptococcus oralis0.52%Streptococcus mitis9.00%Streptococcus pneumoniae1.31%Streptococcus gordonii0.30%Streptococcus alactolyticus0.15%Streptococcus gallolyticus0.16%Streptococcus pyogenes0.45%Granulicatella adiacens0.96%Gemella palaticanis4.41%Gemella cuniculi0.33%Gemella haemolysans1.45%Gemella morbillorum1.05%Megasphaera stantonii1.64%Megasphaera cerevisiae0.22%Megasphaera coli0.56%Megasphaera geminata0.13%Megasphaera paucivorans0.12%Megasphaera sueciensis0.13%Megasphaera massiliensis0.27%Megasphaera elsdenii2.00%Megasphaera hexanoica0.67%Dialister micraerophilus0.13%Dialister invisus2.74%Dialister propionicifaciens0.21%Dialister hominis0.83%Selenomonas ruminantium0.19%Selenomonas sputigena0.47%Selenomonas noxia0.18%Selenomonas flueggei2.65%Selenomonas dianae0.51%Selenomonas infelix0.15%Eubacterium xylanophilum0.17%Eubacterium ramulus4.66%Eubacterium multiforme0.17%Eubacterium barkeri0.18%Eubacterium callanderi0.24%Eubacterium limosum0.95%Parvimonas micra9.43%Peptostreptococcus anaerobius2.27%Peptostreptococcus canis0.15%Peptostreptococcus stomatis0.39%Mogibacterium pumilum0.94%Mogibacterium timidum3.39%Mogibacterium neglectum0.21%Mogibacterium diversum0.37%Mogibacterium vescum0.37%Eubacterium pyruvativorans0.40%Fusobacterium perfoetens5.49%Fusobacterium russii2.54%Fusobacterium periodonticum0.38%Fusobacterium nucleatum9.86%Fusobacterium simiae0.63%Campylobacter sputorum0.68%Campylobacter gracilis0.83%Campylobacter rectus0.16%Campylobacter showae0.93%Campylobacter hyointestinalis0.76%Neisseria macacae0.22%Neisseria flavescens0.69%Ottowia thiooxydans0.18%Ottowia flava0.67%Ottowia beijingensis0.33%Porphyromonas gingivalis3.42%Porphyromonas catoniae0.41%Porphyromonas macacae0.46%Porphyromonas gingivicanis0.24%Tannerella forsythia1.19%Capnocytophaga gingivalis0.22%Pyramidobacter piscolens1.24%Streptococcus downei4.86%Streptococcus salivarius2.41%Branch length scale: 0.8774 substitutions/site across the 500-unit horizontal axis. Root arbitrary.

Public raw read files

Files contain the original full run. Downloads are hosted by ENA.

FileType / bytesMD5
SRR12522735_1.fastq.gzFASTQ / 687054878776a48083af8ce6ffe50e423defccfef

Analysis provenance

marker16S
pipelineArchaions v18.8
read_samplingFirst available reads, not random subsampling
input_sha256ccb210107a300e3840a4c4b0e94ceae9827fcbb4740e4bf6630b1469190cbf04
median_read_length1421.0
reads_in_length_window28585
length_window_bp[1200, 2000]
quality_scoresUnavailable in fetched FASTA; no measured quality filtering
classifier_stats{"marker": "16s", "pipeline": "v18.8", "gpu": "RTX-PRO-6000", "input_path": "volume-fastq-gz", "reads_in": 30000, "reads_skipped": 12085, "elapsed_seconds": 2.2942764900000157, "reads_submitted": 30000, "reads_after_filter": 30000, "adapter_scan": {"sampled": 200, "matched": 4, "fraction": 0.02, "adapters_found": {"BC_FLANK": 4}, "should_trim": false}, "porechop_ran": false, "porechop_reason": "fast-path: no adapter trimming required"}

Full public metadata

All retrieved values are included. Blank source values are displayed as “Not reported”. Expand the sections below to inspect the full record.

ENA run fields (195)
run_accessionSRR12522735
ageNot reported
alignedNot reported
altitudeNot reported
assembly_qualityNot reported
assembly_softwareNot reported
bam_asperaNot reported
bam_bytesNot reported
bam_file_roleNot reported
bam_ftpNot reported
bam_galaxyNot reported
bam_md5Not reported
base_count655372195
binning_softwareNot reported
bio_materialNot reported
bisulfite_protocolNot reported
broad_scale_environmental_contextNot reported
broker_nameNot reported
cage_protocolNot reported
cell_lineNot reported
cell_typeNot reported
center_nameSUB7951364
checklistNot reported
chip_ab_providerNot reported
chip_protocolNot reported
chip_targetNot reported
collected_byNot reported
collection_datenot applicable
collection_date_endNot reported
collection_date_startNot reported
completeness_scoreNot reported
contamination_scoreNot reported
control_experimentNot reported
countryUnited Arab Emirates
cultivarNot reported
culture_collectionNot reported
datahubdcc_metagenome
depthNot reported
descriptionMinION sequencing: metagenomic
dev_stageNot reported
diseaseNot reported
dnase_protocolNot reported
ecotypeNot reported
elevationNot reported
environment_biomeNot reported
environment_featureNot reported
environment_materialNot reported
environmental_mediumNot reported
environmental_sampleNot reported
experiment_accessionSRX9012879
experiment_aliasX89-P
experiment_targetNot reported
experiment_titleMinION sequencing: metagenomic
experimental_factorNot reported
experimental_protocolNot reported
extraction_protocolNot reported
faang_library_selectionNot reported
fastq_asperafasp.sra.ebi.ac.uk:/vol1/fastq/SRR125/035/SRR12522735/SRR12522735_1.fastq.gz
fastq_bytes687054878
fastq_file_roleGENERATED_FILE
fastq_ftpftp.sra.ebi.ac.uk/vol1/fastq/SRR125/035/SRR12522735/SRR12522735_1.fastq.gz
fastq_galaxyftp.sra.ebi.ac.uk/vol1/fastq/SRR125/035/SRR12522735/SRR12522735_1.fastq.gz
fastq_md5776a48083af8ce6ffe50e423defccfef
file_locationNot reported
first_created2021-10-01
first_public2021-10-01
germlineNot reported
hi_c_protocolNot reported
hostHomo sapiens
host_body_siteNot reported
host_genotypeNot reported
host_gravidityNot reported
host_growth_conditionsNot reported
host_phenotypeNot reported
host_scientific_nameHomo sapiens
host_sexNot reported
host_statusNot reported
host_tax_id9606
identified_byNot reported
instrument_modelMinION
instrument_platformOXFORD_NANOPORE
investigation_typeNot reported
isolatemixed1
isolation_sourceoral
last_updated2021-10-01
latNot reported
library_construction_protocolNot reported
library_gen_protocolNot reported
library_layoutSINGLE
library_max_fragment_sizeNot reported
library_min_fragment_sizeNot reported
library_nameX89-P
library_pcr_isolation_protocolNot reported
library_prep_dateNot reported
library_prep_date_formatNot reported
library_prep_latitudeNot reported
library_prep_locationNot reported
library_prep_longitudeNot reported
library_selectionPCR
library_sourceGENOMIC
library_strategyAMPLICON
local_environmental_contextNot reported
locationNot reported
location_endNot reported
location_startNot reported
lonNot reported
marine_regionNot reported
mating_typeNot reported
ncbi_reporting_standardMetagenome or environmental
nominal_lengthNot reported
nominal_sdevNot reported
pcr_isolation_protocolNot reported
phNot reported
project_nameNot reported
protocol_labelNot reported
read_count472274
read_strandNot reported
restriction_enzymeNot reported
restriction_enzyme_target_sequenceNot reported
restriction_siteNot reported
rna_integrity_numNot reported
rna_prep_3_protocolNot reported
rna_prep_5_protocolNot reported
rna_purity_230_ratioNot reported
rna_purity_280_ratioNot reported
rt_prep_protocolNot reported
run_aliasX89.fastq
run_dateNot reported
salinityNot reported
sample_accessionSAMN15887602
sample_aliasX89
sample_capture_statusNot reported
sample_collectionNot reported
sample_descriptionMetagenome or environmental sample from human oral metagenome
sample_materialNot reported
sample_prep_intervalNot reported
sample_prep_interval_unitsNot reported
sample_storageNot reported
sample_storage_processingNot reported
sample_titleMetagenome or environmental sample from human oral metagenome
sampling_campaignNot reported
sampling_platformNot reported
sampling_siteNot reported
scientific_namehuman oral metagenome
secondary_projectNot reported
secondary_sample_accessionSRS7263891
secondary_study_accessionSRP278853
sequencing_dateNot reported
sequencing_date_formatNot reported
sequencing_locationNot reported
sequencing_longitudeNot reported
sequencing_methodNot reported
sequencing_primer_catalogNot reported
sequencing_primer_lotNot reported
sequencing_primer_providerNot reported
serotypeNot reported
serovarNot reported
sexNot reported
specimen_voucherNot reported
sra_asperaNot reported
sra_bytesNot reported
sra_file_roleNot reported
sra_ftpNot reported
sra_galaxyNot reported
sra_md5Not reported
statuspublic
strainmixed
study_accessionPRJNA658726
study_aliasPRJNA658726
study_titleThe impact of smoking different tobacco types on the subgingival microbiome and periodontal health: a pilot study
sub_speciesNot reported
sub_strainNot reported
submission_accessionSRA1117712
submission_toolNot reported
submitted_asperaNot reported
submitted_bytesNot reported
submitted_file_roleNot reported
submitted_formatNot reported
submitted_ftpNot reported
submitted_galaxyNot reported
submitted_host_sexNot reported
submitted_md5Not reported
submitted_read_typeNot reported
surveillance_targetNot reported
tagNot reported
target_geneNot reported
tax_id447426
tax_lineage1;2787823;12908;408169;410656;447426
taxonomic_classificationNot reported
taxonomic_identity_markerNot reported
temperatureNot reported
tissue_libNot reported
tissue_typeNot reported
transposase_protocolNot reported
varietyNot reported
BioSample record and attributes
aliasX89
titleMetagenome or environmental sample from human oral metagenome
descriptionNot reported
strainmixed
collection_datenot applicable
INSDC secondary accessionSRS7263891
aliquotn9
organismhuman oral metagenome
sample_typeSubgingival
isolatemixed1
NCBI submission packageMetagenome.environmental.1.0
periodontitisno-mild
smokingNon-smoker
hostHomo sapiens
geo_loc_nameUnited Arab Emirates
lat_lonmissing
isolation_sourceoral
BioSampleModelMetagenome or environmental
ENA-FIRST-PUBLIC2021-09-30
ENA-LAST-UPDATE2021-09-30
Curated cohort annotations and QC
runSRR12522735
sampleSAMN15887602
studyPRJNA658726
titleMetagenome or environmental sample from human oral metagenome
aliasX89
siteSubgingival
subjectNot reported
phenotypeNot reported
sexNot reported
smokingNon-smoker
periodontitisno-mild
time_pointNot reported
negative_controlFalse
lab_modelFalse
statecomplete
reads_input30000
species_reported76
human_primaryTrue

Model-based taxonomy; research use. This report does not provide a diagnosis or a validated health score.